##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BbeI
BbvCI BciVI BglII BlnI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BstBI BstEII BstPI ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI Hin1I HpaI KasI KspI
MluI MroI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 3497 GGTACC G^GTACC
AccI 3034 GTATAC GT^[AC][GT]AC
AccII 1098 CGCG CG^CG
AflII 2568 CTTAAG C^TTAAG
AflIII 1933 ACGTGT A^C[GA][CT]GT
Asp718I 3497 GGTACC G^GTACC
AvaI 3505 CCCGGG C^[CT]CG[GA]G
BamHI 4222 GGATCC G^GATCC
BbrPI 1932 CACGTG CAC^GTG
BcgI 951 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2568 CTTAAG C^TTAAG
BsaBI 2313 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3429 GGTCTC GGTCTC(1/5)
BsmBI 490 GAGACG CGTCTC(1/5)
BsmI 2356 GCATTC GAATGC(1/-1)
BsoBI 3505 CCCGGG C^[CT]CG[GA]G
BsrBI 1432 CCGCTC CCGCTC(-3/-3)
BsrDI 2921 CATTGC GCAATG(2/0)
Bst1107I 3034 GTATAC GTA^TAC
BstUI 1098 CGCG CG^CG
BstZ17I 3034 GTATAC GTA^TAC
DraI 3991 TTTAAA TTT^AAA
EcoRI 565 GAATTC G^AATTC
EcoT22I 142 ATGCAT ATGCA^T
HgaI 148 GACGC GACGC(5/10)
HincII 3091 GTTGAC GT[CT]^[GA]AC
HindII 3091 GTTGAC GT[CT]^[GA]AC
HindIII 178 AAGCTT A^AGCTT
KpnI 3497 GGTACC GGTAC^C
MamI 2313 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1964 CAATTG C^AATTG
MunI 1964 CAATTG C^AATTG
MvnI 1098 CGCG CG^CG
NsiI 142 ATGCAT ATGCA^T
PmaCI 1932 CACGTG CAC^GTG
PmlI 1932 CACGTG CAC^GTG
PpuMI 48 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 4097 ACCTGGT A^CC[AT]GGT
SmaI 3505 CCCGGG CCC^GGG
StuI 955 AGGCCT AGG^CCT
XmaCI 3505 CCCGGG C^CCGGG
XmaI 3505 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 2724 GTGCAC G^TGCAC
2845 GTGCAC
AocI 2594 CCTCAGG CC^T[ACGT]AGG
4260 CCTAAGG
Aor51HI 1187 AGCGCT AGC^GCT
3629 AGCGCT
ApaLI 2724 GTGCAC G^TGCAC
2845 GTGCAC
BanI 162 GGTGCC G^G[CT][GA]CC
3497 GGTACC
BbsI 1831 GAAGAC GAAGAC(2/6)
68 GTCTTC
BclI 668 TGATCA T^GATCA
1622 TGATCA
BlpI 2800 GCTCAGC GC^T[ACGT]AGC
3632 GCTGAGC
Bpu1102I 2800 GCTCAGC GC^T[ACGT]AGC
3632 GCTGAGC
BpuAI 1831 GAAGAC GAAGAC(2/6)
68 GTCTTC
BsaAI 898 TACGTG [CT]AC^GT[GA]
1932 CACGTG
BssSI 1714 CACGAG CACGAG(-5/-1)
1859 CACGAG
Bsu36I 2594 CCTCAGG CC^T[ACGT]AGG
4260 CCTAAGG
CelII 2800 GCTCAGC GC^T[ACGT]AGC
3632 GCTGAGC
Eco47III 1187 AGCGCT AGC^GCT
3629 AGCGCT
Eco81I 2594 CCTCAGG CC^T[ACGT]AGG
4260 CCTAAGG
FbaI 668 TGATCA T^GATCA
1622 TGATCA
HaeII 1187 AGCGCT [GA]GCGC^[CT]
3629 AGCGCT
NspI 1587 GCATGT [GA]CATG^[CT]
3948 ACATGC
ScaI 1869 AGTACT AGT^ACT
2269 AGTACT
XbaI 2516 TCTAGA T^CTAGA
3549 TCTAGA
XcmI 1235 CCACCCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1907 CCATTCCTTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI