################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccIII AclI AcyI AgeI AhdI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BbeI BbvCI BciVI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI Hin1I HpaI KasI KspI MluI MroI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 3497 GGTACC G^GTACC AccI 3034 GTATAC GT^[AC][GT]AC AccII 1098 CGCG CG^CG AflII 2568 CTTAAG C^TTAAG AflIII 1933 ACGTGT A^C[GA][CT]GT Asp718I 3497 GGTACC G^GTACC AvaI 3505 CCCGGG C^[CT]CG[GA]G BamHI 4222 GGATCC G^GATCC BbrPI 1932 CACGTG CAC^GTG BcgI 951 GCAAAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2568 CTTAAG C^TTAAG BsaBI 2313 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3429 GGTCTC GGTCTC(1/5) BsmBI 490 GAGACG CGTCTC(1/5) BsmI 2356 GCATTC GAATGC(1/-1) BsoBI 3505 CCCGGG C^[CT]CG[GA]G BsrBI 1432 CCGCTC CCGCTC(-3/-3) BsrDI 2921 CATTGC GCAATG(2/0) Bst1107I 3034 GTATAC GTA^TAC BstUI 1098 CGCG CG^CG BstZ17I 3034 GTATAC GTA^TAC DraI 3991 TTTAAA TTT^AAA EcoRI 565 GAATTC G^AATTC EcoT22I 142 ATGCAT ATGCA^T HgaI 148 GACGC GACGC(5/10) HincII 3091 GTTGAC GT[CT]^[GA]AC HindII 3091 GTTGAC GT[CT]^[GA]AC HindIII 178 AAGCTT A^AGCTT KpnI 3497 GGTACC GGTAC^C MamI 2313 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1964 CAATTG C^AATTG MunI 1964 CAATTG C^AATTG MvnI 1098 CGCG CG^CG NsiI 142 ATGCAT ATGCA^T PmaCI 1932 CACGTG CAC^GTG PmlI 1932 CACGTG CAC^GTG PpuMI 48 GGGTCCC [GA]G^G[AT]CC[CT] SexAI 4097 ACCTGGT A^CC[AT]GGT SmaI 3505 CCCGGG CCC^GGG StuI 955 AGGCCT AGG^CCT XmaCI 3505 CCCGGG C^CCGGG XmaI 3505 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 2724 GTGCAC G^TGCAC 2845 GTGCAC AocI 2594 CCTCAGG CC^T[ACGT]AGG 4260 CCTAAGG Aor51HI 1187 AGCGCT AGC^GCT 3629 AGCGCT ApaLI 2724 GTGCAC G^TGCAC 2845 GTGCAC BanI 162 GGTGCC G^G[CT][GA]CC 3497 GGTACC BbsI 1831 GAAGAC GAAGAC(2/6) 68 GTCTTC BclI 668 TGATCA T^GATCA 1622 TGATCA BlpI 2800 GCTCAGC GC^T[ACGT]AGC 3632 GCTGAGC Bpu1102I 2800 GCTCAGC GC^T[ACGT]AGC 3632 GCTGAGC BpuAI 1831 GAAGAC GAAGAC(2/6) 68 GTCTTC BsaAI 898 TACGTG [CT]AC^GT[GA] 1932 CACGTG BssSI 1714 CACGAG CACGAG(-5/-1) 1859 CACGAG Bsu36I 2594 CCTCAGG CC^T[ACGT]AGG 4260 CCTAAGG CelII 2800 GCTCAGC GC^T[ACGT]AGC 3632 GCTGAGC Eco47III 1187 AGCGCT AGC^GCT 3629 AGCGCT Eco81I 2594 CCTCAGG CC^T[ACGT]AGG 4260 CCTAAGG FbaI 668 TGATCA T^GATCA 1622 TGATCA HaeII 1187 AGCGCT [GA]GCGC^[CT] 3629 AGCGCT NspI 1587 GCATGT [GA]CATG^[CT] 3948 ACATGC ScaI 1869 AGTACT AGT^ACT 2269 AGTACT XbaI 2516 TCTAGA T^CTAGA 3549 TCTAGA XcmI 1235 CCACCCATGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1907 CCATTCCTTATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI