##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        ApaI        AscI        AseI        AsnI        
AspEI       AspI        AviII       AvrII       BbeI        
BbvCI       BciVI       BglII       BlnI        BsaHI       
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrGI       
BssHII      BstBI       BstEII      BstPI       ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoO65I     EcoRV       FseI        
FspI        Hin1I       HpaI        KasI        KspI        
MluI        MroI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

Acc65I       3497  GGTACC               G^GTACC
AccI         3034  GTATAC               GT^[AC][GT]AC
AccII        1098  CGCG                 CG^CG
AflII        2568  CTTAAG               C^TTAAG
AflIII       1933  ACGTGT               A^C[GA][CT]GT
Asp718I      3497  GGTACC               G^GTACC
AvaI         3505  CCCGGG               C^[CT]CG[GA]G
BamHI        4222  GGATCC               G^GATCC
BbrPI        1932  CACGTG               CAC^GTG
BcgI          951  GCAAAGGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         2568  CTTAAG               C^TTAAG
BsaBI        2313  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3429  GGTCTC               GGTCTC(1/5)
BsmBI         490  GAGACG               CGTCTC(1/5)
BsmI         2356  GCATTC               GAATGC(1/-1)
BsoBI        3505  CCCGGG               C^[CT]CG[GA]G
BsrBI        1432  CCGCTC               CCGCTC(-3/-3)
BsrDI        2921  CATTGC               GCAATG(2/0)
Bst1107I     3034  GTATAC               GTA^TAC
BstUI        1098  CGCG                 CG^CG
BstZ17I      3034  GTATAC               GTA^TAC
DraI         3991  TTTAAA               TTT^AAA
EcoRI         565  GAATTC               G^AATTC
EcoT22I       142  ATGCAT               ATGCA^T
HgaI          148  GACGC                GACGC(5/10)
HincII       3091  GTTGAC               GT[CT]^[GA]AC
HindII       3091  GTTGAC               GT[CT]^[GA]AC
HindIII       178  AAGCTT               A^AGCTT
KpnI         3497  GGTACC               GGTAC^C
MamI         2313  GATGTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1964  CAATTG               C^AATTG
MunI         1964  CAATTG               C^AATTG
MvnI         1098  CGCG                 CG^CG
NsiI          142  ATGCAT               ATGCA^T
PmaCI        1932  CACGTG               CAC^GTG
PmlI         1932  CACGTG               CAC^GTG
PpuMI          48  GGGTCCC              [GA]G^G[AT]CC[CT]
SexAI        4097  ACCTGGT              A^CC[AT]GGT
SmaI         3505  CCCGGG               CCC^GGG
StuI          955  AGGCCT               AGG^CCT
XmaCI        3505  CCCGGG               C^CCGGG
XmaI         3505  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

Alw44I       2724  GTGCAC               G^TGCAC
             2845  GTGCAC              
AocI         2594  CCTCAGG              CC^T[ACGT]AGG
             4260  CCTAAGG             
Aor51HI      1187  AGCGCT               AGC^GCT
             3629  AGCGCT              
ApaLI        2724  GTGCAC               G^TGCAC
             2845  GTGCAC              
BanI          162  GGTGCC               G^G[CT][GA]CC
             3497  GGTACC              
BbsI         1831  GAAGAC               GAAGAC(2/6)
               68  GTCTTC              
BclI          668  TGATCA               T^GATCA
             1622  TGATCA              
BlpI         2800  GCTCAGC              GC^T[ACGT]AGC
             3632  GCTGAGC             
Bpu1102I     2800  GCTCAGC              GC^T[ACGT]AGC
             3632  GCTGAGC             
BpuAI        1831  GAAGAC               GAAGAC(2/6)
               68  GTCTTC              
BsaAI         898  TACGTG               [CT]AC^GT[GA]
             1932  CACGTG              
BssSI        1714  CACGAG               CACGAG(-5/-1)
             1859  CACGAG              
Bsu36I       2594  CCTCAGG              CC^T[ACGT]AGG
             4260  CCTAAGG             
CelII        2800  GCTCAGC              GC^T[ACGT]AGC
             3632  GCTGAGC             
Eco47III     1187  AGCGCT               AGC^GCT
             3629  AGCGCT              
Eco81I       2594  CCTCAGG              CC^T[ACGT]AGG
             4260  CCTAAGG             
FbaI          668  TGATCA               T^GATCA
             1622  TGATCA              
HaeII        1187  AGCGCT               [GA]GCGC^[CT]
             3629  AGCGCT              
NspI         1587  GCATGT               [GA]CATG^[CT]
             3948  ACATGC              
ScaI         1869  AGTACT               AGT^ACT
             2269  AGTACT              
XbaI         2516  TCTAGA               T^CTAGA
             3549  TCTAGA              
XcmI         1235  CCACCCATGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1907  CCATTCCTTATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BalI        BanII       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrFI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       PflMI       
PleI        PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI