##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccIII AgeI Aor51HI
ApaI AscI AseI AsnI Asp718I
AvrII BcgI BlnI BsaI BsaWI
BseAI BsiEI BsiWI BspEI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CpoI EagI EclXI
Eco47III Eco52I EcoO65I EcoT22I FseI
HpaI KpnI MluI MroI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 499 AACGTT AA^CGTT
AflII 2097 CTTAAG C^TTAAG
AhdI 2852 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4557 GTGCAC G^TGCAC
AocI 406 CCTCAGG CC^T[ACGT]AGG
ApaLI 4557 GTGCAC G^TGCAC
AspEI 2852 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4473 TGCGCA TGC^GCA
BbeI 3388 GGCGCC GGCGC^C
BfrI 2097 CTTAAG C^TTAAG
BglI 4189 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 4480 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4480 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 2408 TGTACA T^GTACA
BspDI 382 ATCGAT AT^CGAT
BsrBI 2968 GAGCGG CCGCTC(-3/-3)
BsrGI 2408 TGTACA T^GTACA
Bsu36I 406 CCTCAGG CC^T[ACGT]AGG
CelII 4480 GCTCAGC GC^T[ACGT]AGC
ClaI 382 ATCGAT AT^CGAT
DraI 2623 TTTAAA TTT^AAA
DrdI 2266 GACGCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2852 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 406 CCTCAGG CC^T[ACGT]AGG
EcoRV 648 GATATC GAT^ATC
FspI 4473 TGCGCA TGC^GCA
HaeII 3388 GGCGCC [GA]GCGC^[CT]
HincII 502 GTTGAC GT[CT]^[GA]AC
HindII 502 GTTGAC GT[CT]^[GA]AC
KasI 3388 GGCGCC G^GCGCC
KspI 9 CCGCGG CCGC^GG
NaeI 48 GCCGGC GCC^GGC
NarI 3388 GGCGCC GG^CGCC
NdeI 3118 CATATG CA^TATG
NgoMIV 48 GCCGGC G^CCGGC
NspI 4749 GCATGT [GA]CATG^[CT]
PaeR7I 2806 CTCGAG C^TCGAG
Psp1406I 499 AACGTT AA^CGTT
SacII 9 CCGCGG CCGC^GG
SexAI 2486 ACCAGGT A^CC[AT]GGT
SfoI 3388 GGCGCC GGC^GCC
SspBI 2408 TGTACA T^GTACA
StuI 4428 AGGCCT AGG^CCT
XhoI 2806 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1564 GTCTAC GT^[AC][GT]AC
2281 GTCTAC
AspI 1692 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3262 GACCAAGTC
AvaI 1141 CCCGAG C^[CT]CG[GA]G
2806 CTCGAG
BamHI 1266 GGATCC G^GATCC
4179 GGATCC
BbrPI 18 CACGTG CAC^GTG
1501 CACGTG
BbvCI 840 CCTCAGC CCTCAGC(-5/-2)
3079 GCTGAGG
BcnI 1920 CCCGG CC^[GC]GG
4738 CCCGG
BsaBI 769 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
943 GATGCTCATC
BsoBI 1141 CCCGAG C^[CT]CG[GA]G
2806 CTCGAG
BspHI 1797 TCATGA T^CATGA
2132 TCATGA
BsrDI 3109 GCAATG GCAATG(2/0)
3583 GCAATG
BsrFI 48 GCCGGC [GA]^CCGG[CT]
4593 GCCGGT
BstAPI 2428 GCAGGTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3804 GCAAACCTTGC
BstXI 275 CCAGGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4777 CCAGGGAAATGG
Cfr10I 48 GCCGGC [GA]^CCGG[CT]
4593 GCCGGT
DraIII 897 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1297 CACTTCGTG
EcoNI 1749 CCTGAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4567 CCTGTTACAGG
MamI 769 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
943 GATGCTCATC
MfeI 704 CAATTG C^AATTG
3506 CAATTG
MunI 704 CAATTG C^AATTG
3506 CAATTG
NciI 1920 CCCGG CC^[GC]GG
4738 CCCGG
PflFI 1692 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3262 GACCAAGTC
PmaCI 18 CACGTG CAC^GTG
1501 CACGTG
PmlI 18 CACGTG CAC^GTG
1501 CACGTG
RcaI 1797 TCATGA T^CATGA
2132 TCATGA
SacI 546 GAGCTC GAGCT^C
4294 GAGCTC
SapI 4704 GAAGAGC GCTCTTC(1/4)
4725 GAAGAGC
SspI 756 AATATT AAT^ATT
4613 AATATT
Tth111I 1692 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3262 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BciVI BclI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI