##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AscI AseI AsnI
BamHI BcgI BclI BglII BsiEI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
HindIII HpaI MfeI MluI MunI
NdeI NotI NruI NsiI NspV
PacI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI ScaI SfuI
SnaBI SpeI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1634 GGTACC G^GTACC
AflIII 2045 ACGTGT A^C[GA][CT]GT
AgeI 805 ACCGGT A^CCGGT
Alw44I 1251 GTGCAC G^TGCAC
Aor51HI 3550 AGCGCT AGC^GCT
ApaLI 1251 GTGCAC G^TGCAC
Asp718I 1634 GGTACC G^GTACC
AspI 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3988 GGCGCC GGCGC^C
BbvCI 3132 CCTCAGC CCTCAGC(-5/-2)
BsaBI 3178 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 158 TCATGA T^CATGA
BstEII 1149 GGTGACC G^GT[ACGT]ACC
BstPI 1149 GGTGACC G^GT[ACGT]ACC
DraI 4397 TTTAAA TTT^AAA
DrdI 3121 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3550 AGCGCT AGC^GCT
EcoO65I 1149 GGTGACC G^GT[ACGT]ACC
FseI 3596 GGCCGGCC GGCCGG^CC
HincII 3466 GTCAAC GT[CT]^[GA]AC
HindII 3466 GTCAAC GT[CT]^[GA]AC
KasI 3988 GGCGCC G^GCGCC
KpnI 1634 GGTACC GGTAC^C
KspI 502 CCGCGG CCGC^GG
MamI 3178 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3988 GGCGCC GG^CGCC
NheI 4198 GCTAGC G^CTAGC
PaeR7I 2297 CTCGAG C^TCGAG
PflFI 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 805 ACCGGT A^CCGGT
RcaI 158 TCATGA T^CATGA
SacII 502 CCGCGG CCGC^GG
SexAI 2279 ACCAGGT A^CC[AT]GGT
SfiI 1044 GGCCCCGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3988 GGCGCC GGC^GCC
SgrAI 804 CACCGGTG C[GA]^CCGG[CT]G
Tth111I 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2148 TCTAGA T^CTAGA
XhoI 2297 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 49 GTAGAC GT^[AC][GT]AC
622 GTAGAC
AccIII 2633 TCCGGA T^CCGGA
2950 TCCGGA
AflII 316 CTTAAG C^TTAAG
2650 CTTAAG
AhdI 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACCAGGAGTC
AlwNI 2566 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
4006 CAGGGACTG
Asp700I 813 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2625 GAAGACTTTC
AspEI 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACCAGGAGTC
AviII 794 TGCGCA TGC^GCA
1439 TGCGCA
AvrII 678 CCTAGG C^CTAGG
3423 CCTAGG
BciVI 2666 GGATAC GTATCC(6/5)
735 GTATCC
BfrI 316 CTTAAG C^TTAAG
2650 CTTAAG
BlnI 678 CCTAGG C^CTAGG
3423 CCTAGG
BseAI 2633 TCCGGA T^CCGGA
2950 TCCGGA
BsmI 170 GAATGC GAATGC(1/-1)
2292 GCATTC
BspEI 2633 TCCGGA T^CCGGA
2950 TCCGGA
BssSI 946 CACGAG CACGAG(-5/-1)
1828 CTCGTG
BstYI 648 GGATCT [GA]^GATC[CT]
869 AGATCC
Eam1105I 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACCAGGAGTC
EcoNI 1599 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2146 CCTCTAGAAGG
FspI 794 TGCGCA TGC^GCA
1439 TGCGCA
MflI 648 GGATCT [GA]^GATC[CT]
869 AGATCC
MroI 2633 TCCGGA T^CCGGA
2950 TCCGGA
SacI 1288 GAGCTC GAGCT^C
1717 GAGCTC
SapI 1207 GAAGAGC GCTCTTC(1/4)
3056 GAAGAGC
SbfI 2205 CCTGCAGG CCTGCA^GG
3105 CCTGCAGG
SmaI 3729 CCCGGG CCC^GGG
4116 CCCGGG
Sse8387I 2205 CCTGCAGG CCTGCA^GG
3105 CCTGCAGG
XhoII 648 GGATCT [GA]^GATC[CT]
869 AGATCC
XmaCI 3729 CCCGGG C^CCGGG
4116 CCCGGG
XmaI 3729 CCCGGG C^CCGGG
4116 CCCGGG
XmnI 813 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2625 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI