################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AclI AscI AseI AsnI BamHI BcgI BclI BglII BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FbaI HindIII HpaI MfeI MluI MunI NdeI NotI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SnaBI SpeI SspBI SspI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1634 GGTACC G^GTACC AflIII 2045 ACGTGT A^C[GA][CT]GT AgeI 805 ACCGGT A^CCGGT Alw44I 1251 GTGCAC G^TGCAC Aor51HI 3550 AGCGCT AGC^GCT ApaLI 1251 GTGCAC G^TGCAC Asp718I 1634 GGTACC G^GTACC AspI 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 3988 GGCGCC GGCGC^C BbvCI 3132 CCTCAGC CCTCAGC(-5/-2) BsaBI 3178 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspHI 158 TCATGA T^CATGA BstEII 1149 GGTGACC G^GT[ACGT]ACC BstPI 1149 GGTGACC G^GT[ACGT]ACC DraI 4397 TTTAAA TTT^AAA DrdI 3121 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3550 AGCGCT AGC^GCT EcoO65I 1149 GGTGACC G^GT[ACGT]ACC FseI 3596 GGCCGGCC GGCCGG^CC HincII 3466 GTCAAC GT[CT]^[GA]AC HindII 3466 GTCAAC GT[CT]^[GA]AC KasI 3988 GGCGCC G^GCGCC KpnI 1634 GGTACC GGTAC^C KspI 502 CCGCGG CCGC^GG MamI 3178 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 3988 GGCGCC GG^CGCC NheI 4198 GCTAGC G^CTAGC PaeR7I 2297 CTCGAG C^TCGAG PflFI 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 805 ACCGGT A^CCGGT RcaI 158 TCATGA T^CATGA SacII 502 CCGCGG CCGC^GG SexAI 2279 ACCAGGT A^CC[AT]GGT SfiI 1044 GGCCCCGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3988 GGCGCC GGC^GCC SgrAI 804 CACCGGTG C[GA]^CCGG[CT]G Tth111I 3163 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2148 TCTAGA T^CTAGA XhoI 2297 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 49 GTAGAC GT^[AC][GT]AC 622 GTAGAC AccIII 2633 TCCGGA T^CCGGA 2950 TCCGGA AflII 316 CTTAAG C^TTAAG 2650 CTTAAG AhdI 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACCAGGAGTC AlwNI 2566 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 4006 CAGGGACTG Asp700I 813 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2625 GAAGACTTTC AspEI 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACCAGGAGTC AviII 794 TGCGCA TGC^GCA 1439 TGCGCA AvrII 678 CCTAGG C^CTAGG 3423 CCTAGG BciVI 2666 GGATAC GTATCC(6/5) 735 GTATCC BfrI 316 CTTAAG C^TTAAG 2650 CTTAAG BlnI 678 CCTAGG C^CTAGG 3423 CCTAGG BseAI 2633 TCCGGA T^CCGGA 2950 TCCGGA BsmI 170 GAATGC GAATGC(1/-1) 2292 GCATTC BspEI 2633 TCCGGA T^CCGGA 2950 TCCGGA BssSI 946 CACGAG CACGAG(-5/-1) 1828 CTCGTG BstYI 648 GGATCT [GA]^GATC[CT] 869 AGATCC Eam1105I 305 GACTGACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACCAGGAGTC EcoNI 1599 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2146 CCTCTAGAAGG FspI 794 TGCGCA TGC^GCA 1439 TGCGCA MflI 648 GGATCT [GA]^GATC[CT] 869 AGATCC MroI 2633 TCCGGA T^CCGGA 2950 TCCGGA SacI 1288 GAGCTC GAGCT^C 1717 GAGCTC SapI 1207 GAAGAGC GCTCTTC(1/4) 3056 GAAGAGC SbfI 2205 CCTGCAGG CCTGCA^GG 3105 CCTGCAGG SmaI 3729 CCCGGG CCC^GGG 4116 CCCGGG Sse8387I 2205 CCTGCAGG CCTGCA^GG 3105 CCTGCAGG XhoII 648 GGATCT [GA]^GATC[CT] 869 AGATCC XmaCI 3729 CCCGGG C^CCGGG 4116 CCCGGG XmaI 3729 CCCGGG C^CCGGG 4116 CCCGGG XmnI 813 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2625 GAAGACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI