##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AscI        Asp700I     Asp718I     AspI        
BbrPI       BciVI       BfrI        BsaBI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        EagI        EarI        EclXI       
Eco52I      EcoT22I     FseI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SapI        SbfI        SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        SwaI        TfiI        Tth111I     
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          248  GTCGAC               GT^[AC][GT]AC
AhdI           11  GACCTAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        810  GTGCAC               G^TGCAC
Aor51HI       997  AGCGCT               AGC^GCT
ApaLI         810  GTGCAC               G^TGCAC
AseI         3138  ATTAAT               AT^TAAT
AsnI         3138  ATTAAT               AT^TAAT
AspEI          11  GACCTAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII          13  CCTAGG               C^CTAGG
BalI         2987  TGGCCA               TGG^CCA
BbeI         1813  GGCGCC               GGCGC^C
BcgI         1099  GCAAGATCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1960  TGATCA               T^GATCA
BglII         283  AGATCT               A^GATCT
BlnI           13  CCTAGG               C^CTAGG
BlpI          427  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      427  GCTCAGC              GC^T[ACGT]AGC
BsmI         1934  GAATGC               GAATGC(1/-1)
Bsp1407I      373  TGTACA               T^GTACA
BsrGI         373  TGTACA               T^GTACA
BstAPI        874  GCACATACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        954  GGTCACC              G^GT[ACGT]ACC
BstPI         954  GGTCACC              G^GT[ACGT]ACC
CelII         427  GCTCAGC              GC^T[ACGT]AGC
Eam1105I       11  GACCTAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      997  AGCGCT               AGC^GCT
EcoO65I       954  GGTCACC              G^GT[ACGT]ACC
EcoRI        1376  GAATTC               G^AATTC
FauI         2048  GCGGG                CCCGC(4/6)
FbaI         1960  TGATCA               T^GATCA
HindIII      1599  AAGCTT               A^AGCTT
HpaI          165  GTTAAC               GTT^AAC
KasI         1813  GGCGCC               G^GCGCC
MluNI        2987  TGGCCA               TGG^CCA
MscI         2987  TGGCCA               TGG^CCA
MslI         1997  CATCTACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1813  GGCGCC               GG^CGCC
PaeR7I       1320  CTCGAG               C^TCGAG
PshAI         261  GACTATTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3138  ATTAAT               AT^TAAT
SalI          248  GTCGAC               G^TCGAC
SexAI        2273  ACCAGGT              A^CC[AT]GGT
SfoI         1813  GGCGCC               GGC^GCC
SspBI         373  TGTACA               T^GTACA
StuI         2590  AGGCCT               AGG^CCT
XcmI         2274  CCAGGTCCTAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1320  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          101  GAATTT               [GA]^AATT[CT]
             1376  GAATTC              
AcyI         1659  GACGCC               G[GA]^CG[CT]C
             1813  GGCGCC              
AflIII        605  ACGTGT               A^C[GA][CT]GT
             2002  ACGTGT              
AocI         2512  CCTCAGG              CC^T[ACGT]AGG
             2643  CCTGAGG             
ApaI         1426  GGGCCC               GGGCC^C
             3055  GGGCCC              
ApoI          101  GAATTT               [GA]^AATT[CT]
             1376  GAATTC              
AspHI         693  GAGCAC               G[AT]GC[AT]^C
              810  GTGCAC              
AviII        1900  TGCGCA               TGC^GCA
             2101  TGCGCA              
BamHI        1327  GGATCC               G^GATCC
             2909  GGATCC              
BbsI          924  GAAGAC               GAAGAC(2/6)
              546  GTCTTC              
BpuAI         924  GAAGAC               GAAGAC(2/6)
              546  GTCTTC              
BsaAI        1032  TACGTG               [CT]AC^GT[GA]
             2001  TACGTG              
BsaHI        1659  GACGCC               G[GA]^CG[CT]C
             1813  GGCGCC              
BsaI         1164  GAGACC               GGTCTC(1/5)
              239  GGTCTC              
BsiHKAI       693  GAGCAC               G[AT]GC[AT]^C
              810  GTGCAC              
BsrBI        1148  CCGCTC               CCGCTC(-3/-3)
             1680  GAGCGG              
Bsu36I       2512  CCTCAGG              CC^T[ACGT]AGG
             2643  CCTGAGG             
EaeI         1669  CGGCCA               [CT]^GGCC[GA]
             2987  TGGCCA              
Eco81I       2512  CCTCAGG              CC^T[ACGT]AGG
             2643  CCTGAGG             
EcoRV        1990  GATATC               GAT^ATC
             2846  GATATC              
FspI         1900  TGCGCA               TGC^GCA
             2101  TGCGCA              
Hin1I        1659  GACGCC               G[GA]^CG[CT]C
             1813  GGCGCC              
PvuII         639  CAGCTG               CAG^CTG
             2105  CAGCTG              
ScaI         1333  AGTACT               AGT^ACT
             2737  AGTACT              
SmaI          964  CCCGGG               CCC^GGG
             2775  CCCGGG              
TaqI          249  TCGA                 T^CGA
             1321  TCGA                
TthHB8I       249  TCGA                 T^CGA
             1321  TCGA                
XbaI          217  TCTAGA               T^CTAGA
             2555  TCTAGA              
XmaCI         964  CCCGGG               C^CCGGG
             2775  CCCGGG              
XmaI          964  CCCGGG               C^CCGGG
             2775  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AvaI        AvaII       BanI        BanII       
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EcoNI       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII