##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AscI Asp700I Asp718I AspI
BbrPI BciVI BfrI BsaBI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI EagI EarI EclXI
Eco52I EcoT22I FseI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacI SacII SapI SbfI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tth111I
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 248 GTCGAC GT^[AC][GT]AC
AhdI 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 810 GTGCAC G^TGCAC
Aor51HI 997 AGCGCT AGC^GCT
ApaLI 810 GTGCAC G^TGCAC
AseI 3138 ATTAAT AT^TAAT
AsnI 3138 ATTAAT AT^TAAT
AspEI 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 13 CCTAGG C^CTAGG
BalI 2987 TGGCCA TGG^CCA
BbeI 1813 GGCGCC GGCGC^C
BcgI 1099 GCAAGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1960 TGATCA T^GATCA
BglII 283 AGATCT A^GATCT
BlnI 13 CCTAGG C^CTAGG
BlpI 427 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 427 GCTCAGC GC^T[ACGT]AGC
BsmI 1934 GAATGC GAATGC(1/-1)
Bsp1407I 373 TGTACA T^GTACA
BsrGI 373 TGTACA T^GTACA
BstAPI 874 GCACATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 954 GGTCACC G^GT[ACGT]ACC
BstPI 954 GGTCACC G^GT[ACGT]ACC
CelII 427 GCTCAGC GC^T[ACGT]AGC
Eam1105I 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 997 AGCGCT AGC^GCT
EcoO65I 954 GGTCACC G^GT[ACGT]ACC
EcoRI 1376 GAATTC G^AATTC
FauI 2048 GCGGG CCCGC(4/6)
FbaI 1960 TGATCA T^GATCA
HindIII 1599 AAGCTT A^AGCTT
HpaI 165 GTTAAC GTT^AAC
KasI 1813 GGCGCC G^GCGCC
MluNI 2987 TGGCCA TGG^CCA
MscI 2987 TGGCCA TGG^CCA
MslI 1997 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1813 GGCGCC GG^CGCC
PaeR7I 1320 CTCGAG C^TCGAG
PshAI 261 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3138 ATTAAT AT^TAAT
SalI 248 GTCGAC G^TCGAC
SexAI 2273 ACCAGGT A^CC[AT]GGT
SfoI 1813 GGCGCC GGC^GCC
SspBI 373 TGTACA T^GTACA
StuI 2590 AGGCCT AGG^CCT
XcmI 2274 CCAGGTCCTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1320 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 101 GAATTT [GA]^AATT[CT]
1376 GAATTC
AcyI 1659 GACGCC G[GA]^CG[CT]C
1813 GGCGCC
AflIII 605 ACGTGT A^C[GA][CT]GT
2002 ACGTGT
AocI 2512 CCTCAGG CC^T[ACGT]AGG
2643 CCTGAGG
ApaI 1426 GGGCCC GGGCC^C
3055 GGGCCC
ApoI 101 GAATTT [GA]^AATT[CT]
1376 GAATTC
AspHI 693 GAGCAC G[AT]GC[AT]^C
810 GTGCAC
AviII 1900 TGCGCA TGC^GCA
2101 TGCGCA
BamHI 1327 GGATCC G^GATCC
2909 GGATCC
BbsI 924 GAAGAC GAAGAC(2/6)
546 GTCTTC
BpuAI 924 GAAGAC GAAGAC(2/6)
546 GTCTTC
BsaAI 1032 TACGTG [CT]AC^GT[GA]
2001 TACGTG
BsaHI 1659 GACGCC G[GA]^CG[CT]C
1813 GGCGCC
BsaI 1164 GAGACC GGTCTC(1/5)
239 GGTCTC
BsiHKAI 693 GAGCAC G[AT]GC[AT]^C
810 GTGCAC
BsrBI 1148 CCGCTC CCGCTC(-3/-3)
1680 GAGCGG
Bsu36I 2512 CCTCAGG CC^T[ACGT]AGG
2643 CCTGAGG
EaeI 1669 CGGCCA [CT]^GGCC[GA]
2987 TGGCCA
Eco81I 2512 CCTCAGG CC^T[ACGT]AGG
2643 CCTGAGG
EcoRV 1990 GATATC GAT^ATC
2846 GATATC
FspI 1900 TGCGCA TGC^GCA
2101 TGCGCA
Hin1I 1659 GACGCC G[GA]^CG[CT]C
1813 GGCGCC
PvuII 639 CAGCTG CAG^CTG
2105 CAGCTG
ScaI 1333 AGTACT AGT^ACT
2737 AGTACT
SmaI 964 CCCGGG CCC^GGG
2775 CCCGGG
TaqI 249 TCGA T^CGA
1321 TCGA
TthHB8I 249 TCGA T^CGA
1321 TCGA
XbaI 217 TCTAGA T^CTAGA
2555 TCTAGA
XmaCI 964 CCCGGG C^CCGGG
2775 CCCGGG
XmaI 964 CCCGGG C^CCGGG
2775 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII