################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AscI Asp700I Asp718I AspI BbrPI BciVI BfrI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI EagI EarI EclXI Eco52I EcoT22I FseI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 248 GTCGAC GT^[AC][GT]AC AhdI 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 810 GTGCAC G^TGCAC Aor51HI 997 AGCGCT AGC^GCT ApaLI 810 GTGCAC G^TGCAC AseI 3138 ATTAAT AT^TAAT AsnI 3138 ATTAAT AT^TAAT AspEI 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 13 CCTAGG C^CTAGG BalI 2987 TGGCCA TGG^CCA BbeI 1813 GGCGCC GGCGC^C BcgI 1099 GCAAGATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1960 TGATCA T^GATCA BglII 283 AGATCT A^GATCT BlnI 13 CCTAGG C^CTAGG BlpI 427 GCTCAGC GC^T[ACGT]AGC Bpu1102I 427 GCTCAGC GC^T[ACGT]AGC BsmI 1934 GAATGC GAATGC(1/-1) Bsp1407I 373 TGTACA T^GTACA BsrGI 373 TGTACA T^GTACA BstAPI 874 GCACATACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 954 GGTCACC G^GT[ACGT]ACC BstPI 954 GGTCACC G^GT[ACGT]ACC CelII 427 GCTCAGC GC^T[ACGT]AGC Eam1105I 11 GACCTAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 997 AGCGCT AGC^GCT EcoO65I 954 GGTCACC G^GT[ACGT]ACC EcoRI 1376 GAATTC G^AATTC FauI 2048 GCGGG CCCGC(4/6) FbaI 1960 TGATCA T^GATCA HindIII 1599 AAGCTT A^AGCTT HpaI 165 GTTAAC GTT^AAC KasI 1813 GGCGCC G^GCGCC MluNI 2987 TGGCCA TGG^CCA MscI 2987 TGGCCA TGG^CCA MslI 1997 CATCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1813 GGCGCC GG^CGCC PaeR7I 1320 CTCGAG C^TCGAG PshAI 261 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3138 ATTAAT AT^TAAT SalI 248 GTCGAC G^TCGAC SexAI 2273 ACCAGGT A^CC[AT]GGT SfoI 1813 GGCGCC GGC^GCC SspBI 373 TGTACA T^GTACA StuI 2590 AGGCCT AGG^CCT XcmI 2274 CCAGGTCCTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1320 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcsI 101 GAATTT [GA]^AATT[CT] 1376 GAATTC AcyI 1659 GACGCC G[GA]^CG[CT]C 1813 GGCGCC AflIII 605 ACGTGT A^C[GA][CT]GT 2002 ACGTGT AocI 2512 CCTCAGG CC^T[ACGT]AGG 2643 CCTGAGG ApaI 1426 GGGCCC GGGCC^C 3055 GGGCCC ApoI 101 GAATTT [GA]^AATT[CT] 1376 GAATTC AspHI 693 GAGCAC G[AT]GC[AT]^C 810 GTGCAC AviII 1900 TGCGCA TGC^GCA 2101 TGCGCA BamHI 1327 GGATCC G^GATCC 2909 GGATCC BbsI 924 GAAGAC GAAGAC(2/6) 546 GTCTTC BpuAI 924 GAAGAC GAAGAC(2/6) 546 GTCTTC BsaAI 1032 TACGTG [CT]AC^GT[GA] 2001 TACGTG BsaHI 1659 GACGCC G[GA]^CG[CT]C 1813 GGCGCC BsaI 1164 GAGACC GGTCTC(1/5) 239 GGTCTC BsiHKAI 693 GAGCAC G[AT]GC[AT]^C 810 GTGCAC BsrBI 1148 CCGCTC CCGCTC(-3/-3) 1680 GAGCGG Bsu36I 2512 CCTCAGG CC^T[ACGT]AGG 2643 CCTGAGG EaeI 1669 CGGCCA [CT]^GGCC[GA] 2987 TGGCCA Eco81I 2512 CCTCAGG CC^T[ACGT]AGG 2643 CCTGAGG EcoRV 1990 GATATC GAT^ATC 2846 GATATC FspI 1900 TGCGCA TGC^GCA 2101 TGCGCA Hin1I 1659 GACGCC G[GA]^CG[CT]C 1813 GGCGCC PvuII 639 CAGCTG CAG^CTG 2105 CAGCTG ScaI 1333 AGTACT AGT^ACT 2737 AGTACT SmaI 964 CCCGGG CCC^GGG 2775 CCCGGG TaqI 249 TCGA T^CGA 1321 TCGA TthHB8I 249 TCGA T^CGA 1321 TCGA XbaI 217 TCTAGA T^CTAGA 2555 TCTAGA XmaCI 964 CCCGGG C^CCGGG 2775 CCCGGG XmaI 964 CCCGGG C^CCGGG 2775 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII