##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AclI AflII Aor51HI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BfrI BlnI BlpI Bpu1102I
BsgI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FspI HpaI KpnI
MluI NdeI NheI NotI NruI
NsiI PacI PaeR7I PmeI PshAI
PshBI Psp1406I RcaI RsrII SalI
SbfI ScaI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2513 GACGTC GACGT^C
AflIII 2313 ACATGT A^C[GA][CT]GT
AgeI 1022 ACCGGT A^CCGGT
AhdI 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 99 GTGCAC G^TGCAC
AocI 3510 CCTGAGG CC^T[ACGT]AGG
ApaLI 99 GTGCAC G^TGCAC
AspEI 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1404 GGATCC G^GATCC
BbrPI 1892 CACGTG CAC^GTG
BclI 2415 TGATCA T^GATCA
BglII 3731 AGATCT A^GATCT
BsaI 689 GAGACC GGTCTC(1/5)
BsmBI 533 GAGACG CGTCTC(1/5)
BsmI 2926 GAATGC GAATGC(1/-1)
BspLU11I 2313 ACATGT A^CATGT
BstAPI 224 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2139 TTCGAA TT^CGAA
Bsu36I 3510 CCTGAGG CC^T[ACGT]AGG
DraIII 2350 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2216 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3510 CCTGAGG CC^T[ACGT]AGG
FbaI 2415 TGATCA T^GATCA
FseI 1229 GGCCGGCC GGCCGG^CC
HincII 2471 GTCAAC GT[CT]^[GA]AC
HindII 2471 GTCAAC GT[CT]^[GA]AC
HindIII 3584 AAGCTT A^AGCTT
KspI 3126 CCGCGG CCGC^GG
MfeI 2205 CAATTG C^AATTG
MunI 2205 CAATTG C^AATTG
NaeI 1230 GCCGGC GCC^GGC
NgoMIV 1230 GCCGGC G^CCGGC
NspV 2139 TTCGAA TT^CGAA
PflFI 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1022 ACCGGT A^CCGGT
PmaCI 1892 CACGTG CAC^GTG
PmlI 1892 CACGTG CAC^GTG
PvuI 2505 CGATCG CGAT^CG
SacII 3126 CCGCGG CCGC^GG
SfuI 2139 TTCGAA TT^CGAA
SmaI 528 CCCGGG CCC^GGG
SphI 264 GCATGC GCATG^C
Tth111I 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 528 CCCGGG C^CCGGG
XmaI 528 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 2574 CGCG CG^CG
3127 CGCG
AccIII 1392 TCCGGA T^CCGGA
3045 TCCGGA
BbeI 2073 GGCGCC GGCGC^C
2701 GGCGCC
BbvCI 1851 CCTCAGC CCTCAGC(-5/-2)
377 GCTGAGG
BcgI 91 CGATCAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
101 GCACCCCTGTCG
BseAI 1392 TCCGGA T^CCGGA
3045 TCCGGA
BsiWI 2487 CGTACG C^GTACG
3554 CGTACG
BspEI 1392 TCCGGA T^CCGGA
3045 TCCGGA
BsrBI 1681 CCGCTC CCGCTC(-3/-3)
2054 GAGCGG
BssSI 324 CACGAG CACGAG(-5/-1)
3808 CTCGTG
BstUI 2574 CGCG CG^CG
3127 CGCG
BstXI 237 CCACGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3430 CCAGTGCGGTGG
KasI 2073 GGCGCC G^GCGCC
2701 GGCGCC
MroI 1392 TCCGGA T^CCGGA
3045 TCCGGA
MvnI 2574 CGCG CG^CG
3127 CGCG
NarI 2073 GGCGCC GG^CGCC
2701 GGCGCC
PflMI 272 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCAATCCTTGG
SacI 518 GAGCTC GAGCT^C
862 GAGCTC
SapI 473 GAAGAGC GCTCTTC(1/4)
2801 GCTCTTC
SexAI 943 ACCTGGT A^CC[AT]GGT
1623 ACCTGGT
SfoI 2073 GGCGCC GGC^GCC
2701 GGCGCC
StuI 760 AGGCCT AGG^CCT
3050 AGGCCT
Van91I 272 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCAATCCTTGG
XcmI 1254 CCATGCTGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3083 CCAGGCTGACCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII