################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AclI AflII Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BfrI BlnI BlpI Bpu1102I BsgI Bsp1407I BspDI BspHI BsrGI BssHII Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FspI HpaI KpnI MluI NdeI NheI NotI NruI NsiI PacI PaeR7I PmeI PshAI PshBI Psp1406I RcaI RsrII SalI SbfI ScaI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 2513 GACGTC GACGT^C AflIII 2313 ACATGT A^C[GA][CT]GT AgeI 1022 ACCGGT A^CCGGT AhdI 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 99 GTGCAC G^TGCAC AocI 3510 CCTGAGG CC^T[ACGT]AGG ApaLI 99 GTGCAC G^TGCAC AspEI 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1404 GGATCC G^GATCC BbrPI 1892 CACGTG CAC^GTG BclI 2415 TGATCA T^GATCA BglII 3731 AGATCT A^GATCT BsaI 689 GAGACC GGTCTC(1/5) BsmBI 533 GAGACG CGTCTC(1/5) BsmI 2926 GAATGC GAATGC(1/-1) BspLU11I 2313 ACATGT A^CATGT BstAPI 224 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2139 TTCGAA TT^CGAA Bsu36I 3510 CCTGAGG CC^T[ACGT]AGG DraIII 2350 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2216 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 940 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 3510 CCTGAGG CC^T[ACGT]AGG FbaI 2415 TGATCA T^GATCA FseI 1229 GGCCGGCC GGCCGG^CC HincII 2471 GTCAAC GT[CT]^[GA]AC HindII 2471 GTCAAC GT[CT]^[GA]AC HindIII 3584 AAGCTT A^AGCTT KspI 3126 CCGCGG CCGC^GG MfeI 2205 CAATTG C^AATTG MunI 2205 CAATTG C^AATTG NaeI 1230 GCCGGC GCC^GGC NgoMIV 1230 GCCGGC G^CCGGC NspV 2139 TTCGAA TT^CGAA PflFI 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1022 ACCGGT A^CCGGT PmaCI 1892 CACGTG CAC^GTG PmlI 1892 CACGTG CAC^GTG PvuI 2505 CGATCG CGAT^CG SacII 3126 CCGCGG CCGC^GG SfuI 2139 TTCGAA TT^CGAA SmaI 528 CCCGGG CCC^GGG SphI 264 GCATGC GCATG^C Tth111I 383 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 528 CCCGGG C^CCGGG XmaI 528 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 2574 CGCG CG^CG 3127 CGCG AccIII 1392 TCCGGA T^CCGGA 3045 TCCGGA BbeI 2073 GGCGCC GGCGC^C 2701 GGCGCC BbvCI 1851 CCTCAGC CCTCAGC(-5/-2) 377 GCTGAGG BcgI 91 CGATCAGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 101 GCACCCCTGTCG BseAI 1392 TCCGGA T^CCGGA 3045 TCCGGA BsiWI 2487 CGTACG C^GTACG 3554 CGTACG BspEI 1392 TCCGGA T^CCGGA 3045 TCCGGA BsrBI 1681 CCGCTC CCGCTC(-3/-3) 2054 GAGCGG BssSI 324 CACGAG CACGAG(-5/-1) 3808 CTCGTG BstUI 2574 CGCG CG^CG 3127 CGCG BstXI 237 CCACGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3430 CCAGTGCGGTGG KasI 2073 GGCGCC G^GCGCC 2701 GGCGCC MroI 1392 TCCGGA T^CCGGA 3045 TCCGGA MvnI 2574 CGCG CG^CG 3127 CGCG NarI 2073 GGCGCC GG^CGCC 2701 GGCGCC PflMI 272 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3361 CCAATCCTTGG SacI 518 GAGCTC GAGCT^C 862 GAGCTC SapI 473 GAAGAGC GCTCTTC(1/4) 2801 GCTCTTC SexAI 943 ACCTGGT A^CC[AT]GGT 1623 ACCTGGT SfoI 2073 GGCGCC GGC^GCC 2701 GGCGCC StuI 760 AGGCCT AGG^CCT 3050 AGGCCT Van91I 272 CCAGTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3361 CCAATCCTTGG XcmI 1254 CCATGCTGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3083 CCAGGCTGACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII