##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AclI AflII AflIII Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII BbrPI BfrI BglII BsaBI
BsiWI BsmI BspDI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
Eco47III EcoRV EcoT22I FseI FspI
HpaI KpnI MamI MluI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Van91I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2710 GACGTC GACGT^C
AspI 394 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1440 CCTAGG C^CTAGG
BamHI 2681 GGATCC G^GATCC
BbeI 165 GGCGCC GGCGC^C
BlnI 1440 CCTAGG C^CTAGG
BsaAI 2155 TACGTG [CT]AC^GT[GA]
Bsp1407I 1135 TGTACA T^GTACA
BspHI 2063 TCATGA T^CATGA
BsrBI 4014 GAGCGG CCGCTC(-3/-3)
BsrDI 2675 GCAATG GCAATG(2/0)
BsrGI 1135 TGTACA T^GTACA
BstEII 1158 GGTCACC G^GT[ACGT]ACC
BstPI 1158 GGTCACC G^GT[ACGT]ACC
DraI 4143 TTTAAA TTT^AAA
EcoO65I 1158 GGTCACC G^GT[ACGT]ACC
EcoRI 588 GAATTC G^AATTC
HincII 3431 GTCGAC GT[CT]^[GA]AC
HindII 3431 GTCGAC GT[CT]^[GA]AC
HindIII 3227 AAGCTT A^AGCTT
KasI 165 GGCGCC G^GCGCC
MfeI 911 CAATTG C^AATTG
MunI 911 CAATTG C^AATTG
NarI 165 GGCGCC GG^CGCC
NheI 2620 GCTAGC G^CTAGC
PflFI 394 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2063 TCATGA T^CATGA
SalI 3431 GTCGAC G^TCGAC
SfoI 165 GGCGCC GGC^GCC
SspBI 1135 TGTACA T^GTACA
Tth111I 394 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 383 TCCGGA T^CCGGA
2111 TCCGGA
AgeI 1619 ACCGGT A^CCGGT
3152 ACCGGT
AhdI 2251 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACTAGCGGTC
AocI 1330 CCTCAGG CC^T[ACGT]AGG
1816 CCTCAGG
AspEI 2251 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACTAGCGGTC
BalI 1251 TGGCCA TGG^CCA
3600 TGGCCA
BbsI 814 GAAGAC GAAGAC(2/6)
3250 GAAGAC
BbvCI 1969 GCTGAGG CCTCAGC(-5/-2)
3332 GCTGAGG
BcgI 369 CGAGACCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1527 GCAGTCCTTTCG
BciVI 2694 GTATCC GTATCC(6/5)
2933 GTATCC
BclI 2381 TGATCA T^GATCA
2951 TGATCA
BpuAI 814 GAAGAC GAAGAC(2/6)
3250 GAAGAC
BseAI 383 TCCGGA T^CCGGA
2111 TCCGGA
BsiEI 179 CGGCCG CG[GA][CT]^CG
204 CGGCCG
BspEI 383 TCCGGA T^CCGGA
2111 TCCGGA
Bsu36I 1330 CCTCAGG CC^T[ACGT]AGG
1816 CCTCAGG
DrdI 699 GACCTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2170 GACTGCTATGTC
EagI 179 CGGCCG C^GGCCG
204 CGGCCG
Eam1105I 2251 GACTATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3537 GACTAGCGGTC
EclXI 179 CGGCCG C^GGCCG
204 CGGCCG
Eco52I 179 CGGCCG C^GGCCG
204 CGGCCG
Eco81I 1330 CCTCAGG CC^T[ACGT]AGG
1816 CCTCAGG
FbaI 2381 TGATCA T^GATCA
2951 TGATCA
MluNI 1251 TGGCCA TGG^CCA
3600 TGGCCA
MroI 383 TCCGGA T^CCGGA
2111 TCCGGA
MscI 1251 TGGCCA TGG^CCA
3600 TGGCCA
MseI 3659 TTAA T^TAA
4144 TTAA
PinAI 1619 ACCGGT A^CCGGT
3152 ACCGGT
PvuII 1483 CAGCTG CAG^CTG
3103 CAGCTG
SapI 2089 GAAGAGC GCTCTTC(1/4)
2352 GCTCTTC
ScaI 644 AGTACT AGT^ACT
1642 AGTACT
SphI 2603 GCATGC GCATG^C
3830 GCATGC
StuI 1290 AGGCCT AGG^CCT
2654 AGGCCT
Tru9I 3659 TTAA T^TAA
4144 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI SacII
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI