################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII AccIII AclI AcsI AflII ApoI AscI AseI AsnI AspI AviII AvrII BcgI BclI BfrI BlnI BsaBI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssHII ClaI CpoI DraI EcoRI EcoRV FbaI FseI FspI HpaI MamI MfeI MluI MroI MunI NdeI NheI NruI PacI PflFI PmeI PshBI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SgrAI SnaBI SpeI SwaI Tth111I XcmI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 4604 GGTACC G^GTACC AgeI 3419 ACCGGT A^CCGGT Aor51HI 1231 AGCGCT AGC^GCT ApaI 3931 GGGCCC GGGCC^C Asp718I 4604 GGTACC G^GTACC BamHI 2020 GGATCC G^GATCC BbrPI 4441 CACGTG CAC^GTG BciVI 839 GTATCC GTATCC(6/5) BglI 3273 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2725 AGATCT A^GATCT BsaWI 3419 ACCGGT [AT]^CCGG[AT] BsmI 2027 GAATGC GAATGC(1/-1) Bsp1407I 1434 TGTACA T^GTACA BspHI 1891 TCATGA T^CATGA BsrBI 4154 GAGCGG CCGCTC(-3/-3) BsrGI 1434 TGTACA T^GTACA Bst1107I 4798 GTATAC GTA^TAC BstAPI 3605 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 693 TTCGAA TT^CGAA BstEII 3725 GGTGACC G^GT[ACGT]ACC BstPI 3725 GGTGACC G^GT[ACGT]ACC BstZ17I 4798 GTATAC GTA^TAC DraIII 2900 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 1231 AGCGCT AGC^GCT EcoO65I 3725 GGTGACC G^GT[ACGT]ACC EcoT22I 2029 ATGCAT ATGCA^T HincII 799 GTTGAC GT[CT]^[GA]AC HindII 799 GTTGAC GT[CT]^[GA]AC KpnI 4604 GGTACC GGTAC^C NotI 51 GCGGCCGC GC^GGCCGC NsiI 2029 ATGCAT ATGCA^T NspI 3997 GCATGC [GA]CATG^[CT] NspV 693 TTCGAA TT^CGAA PaeR7I 4407 CTCGAG C^TCGAG PinAI 3419 ACCGGT A^CCGGT PmaCI 4441 CACGTG CAC^GTG PmlI 4441 CACGTG CAC^GTG PshAI 161 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1891 TCATGA T^CATGA SapI 907 GCTCTTC GCTCTTC(1/4) SbfI 3274 CCTGCAGG CCTGCA^GG SfuI 693 TTCGAA TT^CGAA SmaI 3928 CCCGGG CCC^GGG SphI 3997 GCATGC GCATG^C Sse8387I 3274 CCTGCAGG CCTGCA^GG SspBI 1434 TGTACA T^GTACA SspI 1624 AATATT AAT^ATT XbaI 3343 TCTAGA T^CTAGA XhoI 4407 CTCGAG C^TCGAG XmaCI 3928 CCCGGG C^CCGGG XmaI 3928 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 2060 GTCTAC GT^[AC][GT]AC 4798 GTATAC AcyI 128 GGCGCC G[GA]^CG[CT]C 212 GGCGCC AflIII 413 ACGTGT A^C[GA][CT]GT 4440 ACACGT AhdI 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAGACAGTC AspEI 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAGACAGTC BbeI 128 GGCGCC GGCGC^C 212 GGCGCC BsaHI 128 GGCGCC G[GA]^CG[CT]C 212 GGCGCC BsaI 351 GAGACC GGTCTC(1/5) 3862 GGTCTC BssSI 1514 CTCGTG CACGAG(-5/-1) 2780 CTCGTG DrdI 2051 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2861 GACCAGTACGTC EagI 52 CGGCCG C^GGCCG 715 CGGCCG Eam1105I 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2770 GACAGACAGTC EclXI 52 CGGCCG C^GGCCG 715 CGGCCG Eco52I 52 CGGCCG C^GGCCG 715 CGGCCG Hin1I 128 GGCGCC G[GA]^CG[CT]C 212 GGCGCC KasI 128 GGCGCC G^GCGCC 212 GGCGCC NaeI 1989 GCCGGC GCC^GGC 3318 GCCGGC NarI 128 GGCGCC GG^CGCC 212 GGCGCC NcoI 1008 CCATGG C^CATGG 3741 CCATGG NgoMIV 1989 GCCGGC G^CCGGC 3318 GCCGGC SfoI 128 GGCGCC GGC^GCC 212 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 128 enzymes) # ######################################### AccII AciI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI