##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
ApoI AscI AseI AsnI AspI
AviII AvrII BcgI BclI BfrI
BlnI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BssHII ClaI
CpoI DraI EcoRI EcoRV FbaI
FseI FspI HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NruI PacI PflFI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SgrAI SnaBI SpeI
SwaI Tth111I XcmI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 4604 GGTACC G^GTACC
AgeI 3419 ACCGGT A^CCGGT
Aor51HI 1231 AGCGCT AGC^GCT
ApaI 3931 GGGCCC GGGCC^C
Asp718I 4604 GGTACC G^GTACC
BamHI 2020 GGATCC G^GATCC
BbrPI 4441 CACGTG CAC^GTG
BciVI 839 GTATCC GTATCC(6/5)
BglI 3273 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2725 AGATCT A^GATCT
BsaWI 3419 ACCGGT [AT]^CCGG[AT]
BsmI 2027 GAATGC GAATGC(1/-1)
Bsp1407I 1434 TGTACA T^GTACA
BspHI 1891 TCATGA T^CATGA
BsrBI 4154 GAGCGG CCGCTC(-3/-3)
BsrGI 1434 TGTACA T^GTACA
Bst1107I 4798 GTATAC GTA^TAC
BstAPI 3605 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 693 TTCGAA TT^CGAA
BstEII 3725 GGTGACC G^GT[ACGT]ACC
BstPI 3725 GGTGACC G^GT[ACGT]ACC
BstZ17I 4798 GTATAC GTA^TAC
DraIII 2900 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1231 AGCGCT AGC^GCT
EcoO65I 3725 GGTGACC G^GT[ACGT]ACC
EcoT22I 2029 ATGCAT ATGCA^T
HincII 799 GTTGAC GT[CT]^[GA]AC
HindII 799 GTTGAC GT[CT]^[GA]AC
KpnI 4604 GGTACC GGTAC^C
NotI 51 GCGGCCGC GC^GGCCGC
NsiI 2029 ATGCAT ATGCA^T
NspI 3997 GCATGC [GA]CATG^[CT]
NspV 693 TTCGAA TT^CGAA
PaeR7I 4407 CTCGAG C^TCGAG
PinAI 3419 ACCGGT A^CCGGT
PmaCI 4441 CACGTG CAC^GTG
PmlI 4441 CACGTG CAC^GTG
PshAI 161 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1891 TCATGA T^CATGA
SapI 907 GCTCTTC GCTCTTC(1/4)
SbfI 3274 CCTGCAGG CCTGCA^GG
SfuI 693 TTCGAA TT^CGAA
SmaI 3928 CCCGGG CCC^GGG
SphI 3997 GCATGC GCATG^C
Sse8387I 3274 CCTGCAGG CCTGCA^GG
SspBI 1434 TGTACA T^GTACA
SspI 1624 AATATT AAT^ATT
XbaI 3343 TCTAGA T^CTAGA
XhoI 4407 CTCGAG C^TCGAG
XmaCI 3928 CCCGGG C^CCGGG
XmaI 3928 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 2060 GTCTAC GT^[AC][GT]AC
4798 GTATAC
AcyI 128 GGCGCC G[GA]^CG[CT]C
212 GGCGCC
AflIII 413 ACGTGT A^C[GA][CT]GT
4440 ACACGT
AhdI 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAGACAGTC
AspEI 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAGACAGTC
BbeI 128 GGCGCC GGCGC^C
212 GGCGCC
BsaHI 128 GGCGCC G[GA]^CG[CT]C
212 GGCGCC
BsaI 351 GAGACC GGTCTC(1/5)
3862 GGTCTC
BssSI 1514 CTCGTG CACGAG(-5/-1)
2780 CTCGTG
DrdI 2051 GACTTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACCAGTACGTC
EagI 52 CGGCCG C^GGCCG
715 CGGCCG
Eam1105I 2501 GACCACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACAGACAGTC
EclXI 52 CGGCCG C^GGCCG
715 CGGCCG
Eco52I 52 CGGCCG C^GGCCG
715 CGGCCG
Hin1I 128 GGCGCC G[GA]^CG[CT]C
212 GGCGCC
KasI 128 GGCGCC G^GCGCC
212 GGCGCC
NaeI 1989 GCCGGC GCC^GGC
3318 GCCGGC
NarI 128 GGCGCC GG^CGCC
212 GGCGCC
NcoI 1008 CCATGG C^CATGG
3741 CCATGG
NgoMIV 1989 GCCGGC G^CCGGC
3318 GCCGGC
SfoI 128 GGCGCC GGC^GCC
212 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI