##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AviII BamHI
BglII BlpI Bpu1102I BsaBI BspDI
Bst1107I BstEII BstPI BstZ17I CelII
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
FseI FspI HpaI MamI MluI
NaeI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PmeI PshAI
PshBI RsrII SacI SalI SapI
SbfI SfiI SgrAI SphI Sse8387I
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3391 GGTACC G^GTACC
AccIII 2752 TCCGGA T^CCGGA
AgeI 3931 ACCGGT A^CCGGT
AocI 2335 CCTGAGG CC^T[ACGT]AGG
Asp718I 3391 GGTACC G^GTACC
AvrII 3925 CCTAGG C^CTAGG
BciVI 434 GGATAC GTATCC(6/5)
BclI 657 TGATCA T^GATCA
BglI 107 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3925 CCTAGG C^CTAGG
BseAI 2752 TCCGGA T^CCGGA
BsiEI 2121 CGATCG CG[GA][CT]^CG
BsiWI 3439 CGTACG C^GTACG
BsmBI 1404 GAGACG CGTCTC(1/5)
BspEI 2752 TCCGGA T^CCGGA
BsrFI 3931 ACCGGT [GA]^CCGG[CT]
BssHII 29 GCGCGC G^CGCGC
BssSI 2381 CTCGTG CACGAG(-5/-1)
BstAPI 2213 GCATCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1718 TTCGAA TT^CGAA
BstYI 2109 GGATCT [GA]^GATC[CT]
Bsu36I 2335 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3931 ACCGGT [GA]^CCGG[CT]
DraI 1429 TTTAAA TTT^AAA
Eco81I 2335 CCTGAGG CC^T[ACGT]AGG
EcoRI 2765 GAATTC G^AATTC
FbaI 657 TGATCA T^GATCA
HindIII 383 AAGCTT A^AGCTT
KpnI 3391 GGTACC GGTAC^C
KspI 3827 CCGCGG CCGC^GG
MfeI 1752 CAATTG C^AATTG
MflI 2109 GGATCT [GA]^GATC[CT]
MroI 2752 TCCGGA T^CCGGA
MunI 1752 CAATTG C^AATTG
NspV 1718 TTCGAA TT^CGAA
PinAI 3931 ACCGGT A^CCGGT
PvuI 2121 CGATCG CGAT^CG
SacII 3827 CCGCGG CCGC^GG
ScaI 1875 AGTACT AGT^ACT
SexAI 477 ACCAGGT A^CC[AT]GGT
SfuI 1718 TTCGAA TT^CGAA
SpeI 3476 ACTAGT A^CTAGT
SspI 2547 AATATT AAT^ATT
StuI 2043 AGGCCT AGG^CCT
XcmI 1315 CCACATGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2109 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 596 GTCTAC GT^[AC][GT]AC
2535 GTAGAC
AclI 1241 AACGTT AA^CGTT
2225 AACGTT
AflII 1690 CTTAAG C^TTAAG
3640 CTTAAG
AhdI 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1283 GACGTGTGGTC
AlwNI 641 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
2441 CAGGTCCTG
AspEI 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1283 GACGTGTGGTC
AspI 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1482 GACGGAGTC
BbrPI 2660 CACGTG CAC^GTG
3238 CACGTG
BbvCI 1490 CCTCAGC CCTCAGC(-5/-2)
3834 CCTCAGC
BcgI 1039 CGATCAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
724 GCAAACCTATCG
BfrI 1690 CTTAAG C^TTAAG
3640 CTTAAG
BspHI 1294 TCATGA T^CATGA
2367 TCATGA
BspMI 93 ACCTGC ACCTGC(4/8)
2653 ACCTGC
BsrBI 8 GAGCGG CCGCTC(-3/-3)
2594 GAGCGG
Eam1105I 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1283 GACGTGTGGTC
EcoRV 1117 GATATC GAT^ATC
1208 GATATC
EcoT22I 2211 ATGCAT ATGCA^T
2430 ATGCAT
HincII 1665 GTCAAC GT[CT]^[GA]AC
3146 GTCAAC
HindII 1665 GTCAAC GT[CT]^[GA]AC
3146 GTCAAC
NsiI 2211 ATGCAT ATGCA^T
2430 ATGCAT
PflFI 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1482 GACGGAGTC
PflMI 2419 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3454 CCACCCTCTGG
PmaCI 2660 CACGTG CAC^GTG
3238 CACGTG
PmlI 2660 CACGTG CAC^GTG
3238 CACGTG
Psp1406I 1241 AACGTT AA^CGTT
2225 AACGTT
PvuII 2299 CAGCTG CAG^CTG
3837 CAGCTG
RcaI 1294 TCATGA T^CATGA
2367 TCATGA
SmaI 19 CCCGGG CCC^GGG
159 CCCGGG
SnaBI 3437 TACGTA TAC^GTA
4004 TACGTA
Tth111I 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1482 GACGGAGTC
Van91I 2419 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3454 CCACCCTCTGG
XmaCI 19 CCCGGG C^CCGGG
159 CCCGGG
XmaI 19 CCCGGG C^CCGGG
159 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI