################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AatII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AviII BamHI BglII BlpI Bpu1102I BsaBI BspDI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I FseI FspI HpaI MamI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PmeI PshAI PshBI RsrII SacI SalI SapI SbfI SfiI SgrAI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3391 GGTACC G^GTACC AccIII 2752 TCCGGA T^CCGGA AgeI 3931 ACCGGT A^CCGGT AocI 2335 CCTGAGG CC^T[ACGT]AGG Asp718I 3391 GGTACC G^GTACC AvrII 3925 CCTAGG C^CTAGG BciVI 434 GGATAC GTATCC(6/5) BclI 657 TGATCA T^GATCA BglI 107 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3925 CCTAGG C^CTAGG BseAI 2752 TCCGGA T^CCGGA BsiEI 2121 CGATCG CG[GA][CT]^CG BsiWI 3439 CGTACG C^GTACG BsmBI 1404 GAGACG CGTCTC(1/5) BspEI 2752 TCCGGA T^CCGGA BsrFI 3931 ACCGGT [GA]^CCGG[CT] BssHII 29 GCGCGC G^CGCGC BssSI 2381 CTCGTG CACGAG(-5/-1) BstAPI 2213 GCATCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1718 TTCGAA TT^CGAA BstYI 2109 GGATCT [GA]^GATC[CT] Bsu36I 2335 CCTGAGG CC^T[ACGT]AGG Cfr10I 3931 ACCGGT [GA]^CCGG[CT] DraI 1429 TTTAAA TTT^AAA Eco81I 2335 CCTGAGG CC^T[ACGT]AGG EcoRI 2765 GAATTC G^AATTC FbaI 657 TGATCA T^GATCA HindIII 383 AAGCTT A^AGCTT KpnI 3391 GGTACC GGTAC^C KspI 3827 CCGCGG CCGC^GG MfeI 1752 CAATTG C^AATTG MflI 2109 GGATCT [GA]^GATC[CT] MroI 2752 TCCGGA T^CCGGA MunI 1752 CAATTG C^AATTG NspV 1718 TTCGAA TT^CGAA PinAI 3931 ACCGGT A^CCGGT PvuI 2121 CGATCG CGAT^CG SacII 3827 CCGCGG CCGC^GG ScaI 1875 AGTACT AGT^ACT SexAI 477 ACCAGGT A^CC[AT]GGT SfuI 1718 TTCGAA TT^CGAA SpeI 3476 ACTAGT A^CTAGT SspI 2547 AATATT AAT^ATT StuI 2043 AGGCCT AGG^CCT XcmI 1315 CCACATGGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 2109 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 596 GTCTAC GT^[AC][GT]AC 2535 GTAGAC AclI 1241 AACGTT AA^CGTT 2225 AACGTT AflII 1690 CTTAAG C^TTAAG 3640 CTTAAG AhdI 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1283 GACGTGTGGTC AlwNI 641 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG 2441 CAGGTCCTG AspEI 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1283 GACGTGTGGTC AspI 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1482 GACGGAGTC BbrPI 2660 CACGTG CAC^GTG 3238 CACGTG BbvCI 1490 CCTCAGC CCTCAGC(-5/-2) 3834 CCTCAGC BcgI 1039 CGATCAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 724 GCAAACCTATCG BfrI 1690 CTTAAG C^TTAAG 3640 CTTAAG BspHI 1294 TCATGA T^CATGA 2367 TCATGA BspMI 93 ACCTGC ACCTGC(4/8) 2653 ACCTGC BsrBI 8 GAGCGG CCGCTC(-3/-3) 2594 GAGCGG Eam1105I 476 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1283 GACGTGTGGTC EcoRV 1117 GATATC GAT^ATC 1208 GATATC EcoT22I 2211 ATGCAT ATGCA^T 2430 ATGCAT HincII 1665 GTCAAC GT[CT]^[GA]AC 3146 GTCAAC HindII 1665 GTCAAC GT[CT]^[GA]AC 3146 GTCAAC NsiI 2211 ATGCAT ATGCA^T 2430 ATGCAT PflFI 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1482 GACGGAGTC PflMI 2419 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3454 CCACCCTCTGG PmaCI 2660 CACGTG CAC^GTG 3238 CACGTG PmlI 2660 CACGTG CAC^GTG 3238 CACGTG Psp1406I 1241 AACGTT AA^CGTT 2225 AACGTT PvuII 2299 CAGCTG CAG^CTG 3837 CAGCTG RcaI 1294 TCATGA T^CATGA 2367 TCATGA SmaI 19 CCCGGG CCC^GGG 159 CCCGGG SnaBI 3437 TACGTA TAC^GTA 4004 TACGTA Tth111I 460 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1482 GACGGAGTC Van91I 2419 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3454 CCACCCTCTGG XmaCI 19 CCCGGG C^CCGGG 159 CCCGGG XmaI 19 CCCGGG C^CCGGG 159 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI