##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AvrII BamHI BbrPI
BbsI BclI BfrI BglII BlnI
BpuAI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspMI BsrDI BssHII
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoNI
FbaI FseI KspI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfuI
SnaBI Sse8387I StuI SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 321 GGTACC G^GTACC
AcyI 118 GGCGCC G[GA]^CG[CT]C
AflIII 1593 ACATGT A^C[GA][CT]GT
AlwI 38 GGATC GGATC(4/5)
AocI 1024 CCTGAGG CC^T[ACGT]AGG
Asp718I 321 GGTACC G^GTACC
AviII 898 TGCGCA TGC^GCA
BbeI 118 GGCGCC GGCGC^C
BbvCI 1294 GCTGAGG CCTCAGC(-5/-2)
BcgI 98 GCACCAGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1788 GTATCC GTATCC(6/5)
BlpI 191 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 191 GCTCAGC GC^T[ACGT]AGC
BsaAI 1758 TACGTG [CT]AC^GT[GA]
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BsiEI 684 CGGCCG CG[GA][CT]^CG
BspLU11I 1593 ACATGT A^CATGT
BstAPI 890 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 38 GGATCT [GA]^GATC[CT]
Bsu36I 1024 CCTGAGG CC^T[ACGT]AGG
CelII 191 GCTCAGC GC^T[ACGT]AGC
DraIII 2417 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 684 CGGCCG C^GGCCG
EclXI 684 CGGCCG C^GGCCG
Eco52I 684 CGGCCG C^GGCCG
Eco81I 1024 CCTGAGG CC^T[ACGT]AGG
EcoRV 692 GATATC GAT^ATC
EcoT22I 2664 ATGCAT ATGCA^T
FspI 898 TGCGCA TGC^GCA
HaeII 118 GGCGCC [GA]GCGC^[CT]
Hin1I 118 GGCGCC G[GA]^CG[CT]C
HincII 590 GTTAAC GT[CT]^[GA]AC
HindII 590 GTTAAC GT[CT]^[GA]AC
HindIII 406 AAGCTT A^AGCTT
HpaI 590 GTTAAC GTT^AAC
KasI 118 GGCGCC G^GCGCC
KpnI 321 GGTACC GGTAC^C
MflI 38 GGATCT [GA]^GATC[CT]
NarI 118 GGCGCC GG^CGCC
NcoI 2428 CCATGG C^CATGG
NheI 1933 GCTAGC G^CTAGC
NsiI 2664 ATGCAT ATGCA^T
SacI 1019 GAGCTC GAGCT^C
SapI 452 GAAGAGC GCTCTTC(1/4)
ScaI 544 AGTACT AGT^ACT
SfiI 2432 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 118 GGCGCC GGC^GCC
SgrAI 248 CACCGGCG C[GA]^CCGG[CT]G
SpeI 1205 ACTAGT A^CTAGT
XhoII 38 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2067 GTAGAC GT^[AC][GT]AC
2321 GTCTAC
AgeI 1681 ACCGGT A^CCGGT
2500 ACCGGT
Alw44I 1977 GTGCAC G^TGCAC
2271 GTGCAC
ApaLI 1977 GTGCAC G^TGCAC
2271 GTGCAC
BalI 2431 TGGCCA TGG^CCA
2440 TGGCCA
BglI 182 GCCGACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2433 GCCATCCTGGC
BsaBI 2146 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2228 GATTCACATC
Bsp1407I 597 TGTACA T^GTACA
2583 TGTACA
BsrGI 597 TGTACA T^GTACA
2583 TGTACA
BsrI 1706 CCAGT ACTGG(1/-1)
1744 CCAGT
BstEII 565 GGTGACC G^GT[ACGT]ACC
2503 GGTTACC
BstPI 565 GGTGACC G^GT[ACGT]ACC
2503 GGTTACC
BstXI 846 CCAAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2605 CCACACCTCTGG
DpnII 39 GATC ^GATC
979 GATC
EarI 452 GAAGAG CTCTTC(1/4)
826 GAAGAG
EcoO65I 565 GGTGACC G^GT[ACGT]ACC
2503 GGTTACC
EcoRI 434 GAATTC G^AATTC
1406 GAATTC
HgaI 109 GCGTC GACGC(5/10)
997 GCGTC
Ksp632I 452 GAAGAG CTCTTC(1/4)
826 GAAGAG
MaeII 774 ACGT A^CGT
1759 ACGT
MamI 2146 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2228 GATTCACATC
MboI 39 GATC ^GATC
979 GATC
MluNI 2431 TGGCCA TGG^CCA
2440 TGGCCA
MscI 2431 TGGCCA TGG^CCA
2440 TGGCCA
MslI 682 CACGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1980 CACAGGTGTG
NdeII 39 GATC ^GATC
979 GATC
PinAI 1681 ACCGGT A^CCGGT
2500 ACCGGT
PpuMI 515 AGGTCCT [GA]G^G[AT]CC[CT]
2239 GGGTCCT
PstI 257 CTGCAG CTGCA^G
2333 CTGCAG
PvuII 902 CAGCTG CAG^CTG
2002 CAGCTG
Sau3AI 39 GATC ^GATC
979 GATC
SmaI 86 CCCGGG CCC^GGG
114 CCCGGG
SmlI 418 CTTGAG C^T[CT][GA]AG
494 CTCAAG
SphI 2515 GCATGC GCATG^C
2666 GCATGC
SspBI 597 TGTACA T^GTACA
2583 TGTACA
SspI 1164 AATATT AAT^ATT
1851 AATATT
XbaI 1512 TCTAGA T^CTAGA
2818 TCTAGA
XcmI 2205 CCAGGTGGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2428 CCATGGCCATCCTGG
XmaCI 86 CCCGGG C^CCGGG
114 CCCGGG
XmaI 86 CCCGGG C^CCGGG
114 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApaI ApoI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI PflMI
PleI RsaI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I