##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AclI AflII Alw44I Aor51HI ApaI
ApaLI AscI AspI BamHI BbeI
BbrPI BcgI BciVI BfrI BsaI
BsiWI BspDI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraIII Eco47III EcoNI EcoT22I
FseI KasI KspI MluI NaeI
NarI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SphI Sse8387I StuI SwaI Tth111I
Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 276 GACGTC GACGT^C
Acc65I 570 GGTACC G^GTACC
AccIII 1759 TCCGGA T^CCGGA
AflIII 2515 ACATGT A^C[GA][CT]GT
AgeI 2078 ACCGGT A^CCGGT
AhdI 3499 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 9 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1313 CCTTAGG CC^T[ACGT]AGG
Asp718I 570 GGTACC G^GTACC
AspEI 3499 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 118 TGCGCA TGC^GCA
AvrII 2726 CCTAGG C^CTAGG
BanI 570 GGTACC G^G[CT][GA]CC
BclI 528 TGATCA T^GATCA
BglII 806 AGATCT A^GATCT
BlnI 2726 CCTAGG C^CTAGG
BlpI 90 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 90 GCTGAGC GC^T[ACGT]AGC
BsaBI 678 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1759 TCCGGA T^CCGGA
BspEI 1759 TCCGGA T^CCGGA
BspLU11I 2515 ACATGT A^CATGT
Bsu36I 1313 CCTTAGG CC^T[ACGT]AGG
CelII 90 GCTGAGC GC^T[ACGT]AGC
DrdI 433 GACATGGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1403 CCATGG C^C[GA][CT]GG
EagI 69 CGGCCG C^GGCCG
Eam1105I 3499 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1051 CTCTTC CTCTTC(1/4)
EclXI 69 CGGCCG C^GGCCG
Eco52I 69 CGGCCG C^GGCCG
Eco81I 1313 CCTTAGG CC^T[ACGT]AGG
EcoRI 2946 GAATTC G^AATTC
EcoRV 4053 GATATC GAT^ATC
FbaI 528 TGATCA T^GATCA
FspI 118 TGCGCA TGC^GCA
HaeII 1398 AGCGCC [GA]GCGC^[CT]
HindIII 688 AAGCTT A^AGCTT
HpaI 3873 GTTAAC GTT^AAC
KpnI 570 GGTACC GGTAC^C
Ksp632I 1051 CTCTTC CTCTTC(1/4)
MamI 678 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1988 CAATTG C^AATTG
MroI 1759 TCCGGA T^CCGGA
MunI 1988 CAATTG C^AATTG
NcoI 1403 CCATGG C^CATGG
NotI 68 GCGGCCGC GC^GGCCGC
PinAI 2078 ACCGGT A^CCGGT
PpuMI 2609 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1845 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1050 GCTCTTC GCTCTTC(1/4)
SfiI 124 GGCCGTGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 922 TACGTA TAC^GTA
SpeI 2815 ACTAGT A^CTAGT
XbaI 2221 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 2731 GTAGAC GT^[AC][GT]AC
3496 GTAGAC
AseI 1113 ATTAAT AT^TAAT
3972 ATTAAT
AsnI 1113 ATTAAT AT^TAAT
3972 ATTAAT
AvaII 178 GGACC G^G[AT]CC
2610 GGTCC
BbsI 1084 GAAGAC GAAGAC(2/6)
3507 GTCTTC
BbvCI 1074 GCTGAGG CCTCAGC(-5/-2)
3318 GCTGAGG
BglI 125 GCCGTGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
610 GCCATGATGGC
BpuAI 1084 GAAGAC GAAGAC(2/6)
3507 GTCTTC
BsaAI 200 CACGTA [CT]AC^GT[GA]
922 TACGTA
BsgI 1168 GTGCAG GTGCAG(16/14)
1631 GTGCAG
BsiEI 69 CGGCCG CG[GA][CT]^CG
306 CGACCG
BsmBI 441 CGTCTC CGTCTC(1/5)
852 CGTCTC
BsmI 2165 GCATTC GAATGC(1/-1)
3205 GCATTC
BspMI 452 ACCTGC ACCTGC(4/8)
1548 GCAGGT
BsrBI 228 CCGCTC CCGCTC(-3/-3)
292 GAGCGG
BsrDI 1431 CATTGC GCAATG(2/0)
1864 CATTGC
BstEII 1308 GGTTACC G^GT[ACGT]ACC
2213 GGTCACC
BstPI 1308 GGTTACC G^GT[ACGT]ACC
2213 GGTCACC
DraI 3056 TTTAAA TTT^AAA
3855 TTTAAA
EcoO65I 1308 GGTTACC G^GT[ACGT]ACC
2213 GGTCACC
PshBI 1113 ATTAAT AT^TAAT
3972 ATTAAT
TaqI 1287 TCGA T^CGA
1416 TCGA
TthHB8I 1287 TCGA T^CGA
1416 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmaCI
XmaI XmnI