##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
Asp718I AspI AvaI AviII AvrII
BanI BbeI BcgI BclI BlnI
BlpI Bpu1102I BsaBI BsaI BsiEI
BsoBI BssHII Bst1107I BstBI BstXI
BstZ17I CelII CpoI EagI EclXI
Eco52I FbaI FseI FspI HindIII
KasI KpnI KspI MamI MluI
NarI NdeI NheI NotI NspV
PacI PaeR7I PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 90 TCCGGA T^CCGGA
AcyI 4017 GACGCC G[GA]^CG[CT]C
AhdI 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 924 CCTTAGG CC^T[ACGT]AGG
ApaI 4144 GGGCCC GGGCC^C
AspEI 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4349 TGGCCA TGG^CCA
BbrPI 3424 CACGTG CAC^GTG
BbvCI 2339 GCTGAGG CCTCAGC(-5/-2)
BciVI 2799 GTATCC GTATCC(6/5)
BcnI 3320 CCGGG CC^[GC]GG
BglI 792 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 2415 CTCCAG CTGGAG(16/14)
BsaHI 4017 GACGCC G[GA]^CG[CT]C
BsaWI 90 TCCGGA [AT]^CCGG[AT]
BseAI 90 TCCGGA T^CCGGA
BsiWI 2706 CGTACG C^GTACG
BsmBI 2665 CGTCTC CGTCTC(1/5)
Bsp1407I 1448 TGTACA T^GTACA
BspEI 90 TCCGGA T^CCGGA
BspHI 828 TCATGA T^CATGA
BspLU11I 3554 ACATGT A^CATGT
BsrGI 1448 TGTACA T^GTACA
BssSI 1109 CACGAG CACGAG(-5/-1)
BstAPI 4107 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 924 CCTTAGG CC^T[ACGT]AGG
DrdI 2657 GACAGTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 924 CCTTAGG CC^T[ACGT]AGG
EcoNI 4120 CCTTCAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1056 GATATC GAT^ATC
EcoT22I 4342 ATGCAT ATGCA^T
Hin1I 4017 GACGCC G[GA]^CG[CT]C
MfeI 3575 CAATTG C^AATTG
MluNI 4349 TGGCCA TGG^CCA
MroI 90 TCCGGA T^CCGGA
MscI 4349 TGGCCA TGG^CCA
MunI 3575 CAATTG C^AATTG
NaeI 620 GCCGGC GCC^GGC
NciI 3320 CCGGG CC^[GC]GG
NgoMIV 620 GCCGGC G^CCGGC
NruI 66 TCGCGA TCG^CGA
NsiI 4342 ATGCAT ATGCA^T
PflMI 549 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3424 CACGTG CAC^GTG
PmlI 3424 CACGTG CAC^GTG
PshAI 2120 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 828 TCATGA T^CATGA
SacI 2309 GAGCTC GAGCT^C
SexAI 104 ACCTGGT A^CC[AT]GGT
SspBI 1448 TGTACA T^GTACA
StuI 790 AGGCCT AGG^CCT
SwaI 3587 ATTTAAAT ATTT^AAAT
Van91I 549 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3518 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 254 CTTAAG C^TTAAG
802 CTTAAG
Alw44I 2014 GTGCAC G^TGCAC
4286 GTGCAC
AlwNI 762 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
3713 CAGTTACTG
Aor51HI 1453 AGCGCT AGC^GCT
2058 AGCGCT
ApaLI 2014 GTGCAC G^TGCAC
4286 GTGCAC
AseI 1847 ATTAAT AT^TAAT
3224 ATTAAT
AsnI 1847 ATTAAT AT^TAAT
3224 ATTAAT
Asp700I 1218 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3510 GAACGCCTTC
BamHI 1381 GGATCC G^GATCC
4009 GGATCC
BanII 2309 GAGCTC G[GA]GC[CT]^C
4144 GGGCCC
BfrI 254 CTTAAG C^TTAAG
802 CTTAAG
BspMI 1477 ACCTGC ACCTGC(4/8)
2540 GCAGGT
BsrBI 33 GAGCGG CCGCTC(-3/-3)
1565 GAGCGG
BsrDI 1332 CATTGC GCAATG(2/0)
2826 GCAATG
BsrFI 620 GCCGGC [GA]^CCGG[CT]
1776 GCCGGT
BstEII 1297 GGTGACC G^GT[ACGT]ACC
3906 GGTAACC
BstPI 1297 GGTGACC G^GT[ACGT]ACC
3906 GGTAACC
Cfr10I 620 GCCGGC [GA]^CCGG[CT]
1776 GCCGGT
EaeI 1699 CGGCCA [CT]^GGCC[GA]
4349 TGGCCA
Eco47III 1453 AGCGCT AGC^GCT
2058 AGCGCT
EcoO65I 1297 GGTGACC G^GT[ACGT]ACC
3906 GGTAACC
EcoRI 2519 GAATTC G^AATTC
3873 GAATTC
FauI 1567 GCGGG CCCGC(4/6)
3732 GCGGG
HpaI 3846 GTTAAC GTT^AAC
4315 GTTAAC
NcoI 1246 CCATGG C^CATGG
3256 CCATGG
PshBI 1847 ATTAAT AT^TAAT
3224 ATTAAT
ScaI 3020 AGTACT AGT^ACT
3743 AGTACT
XmnI 1218 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3510 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI ApoI AspHI
AvaII BbsI BbvI BfaI BglII
BmyI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspDI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I ClaI DdeI
DpnII DraI DraII DraIII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII