################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AclI AgeI AscI Asp718I AspI AvaI AviII AvrII BanI BbeI BcgI BclI BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsoBI BssHII Bst1107I BstBI BstXI BstZ17I CelII CpoI EagI EclXI Eco52I FbaI FseI FspI HindIII KasI KpnI KspI MamI MluI NarI NdeI NheI NotI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccIII 90 TCCGGA T^CCGGA AcyI 4017 GACGCC G[GA]^CG[CT]C AhdI 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 924 CCTTAGG CC^T[ACGT]AGG ApaI 4144 GGGCCC GGGCC^C AspEI 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 4349 TGGCCA TGG^CCA BbrPI 3424 CACGTG CAC^GTG BbvCI 2339 GCTGAGG CCTCAGC(-5/-2) BciVI 2799 GTATCC GTATCC(6/5) BcnI 3320 CCGGG CC^[GC]GG BglI 792 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 2415 CTCCAG CTGGAG(16/14) BsaHI 4017 GACGCC G[GA]^CG[CT]C BsaWI 90 TCCGGA [AT]^CCGG[AT] BseAI 90 TCCGGA T^CCGGA BsiWI 2706 CGTACG C^GTACG BsmBI 2665 CGTCTC CGTCTC(1/5) Bsp1407I 1448 TGTACA T^GTACA BspEI 90 TCCGGA T^CCGGA BspHI 828 TCATGA T^CATGA BspLU11I 3554 ACATGT A^CATGT BsrGI 1448 TGTACA T^GTACA BssSI 1109 CACGAG CACGAG(-5/-1) BstAPI 4107 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 924 CCTTAGG CC^T[ACGT]AGG DrdI 2657 GACAGTAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 242 GACGCGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 924 CCTTAGG CC^T[ACGT]AGG EcoNI 4120 CCTTCAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1056 GATATC GAT^ATC EcoT22I 4342 ATGCAT ATGCA^T Hin1I 4017 GACGCC G[GA]^CG[CT]C MfeI 3575 CAATTG C^AATTG MluNI 4349 TGGCCA TGG^CCA MroI 90 TCCGGA T^CCGGA MscI 4349 TGGCCA TGG^CCA MunI 3575 CAATTG C^AATTG NaeI 620 GCCGGC GCC^GGC NciI 3320 CCGGG CC^[GC]GG NgoMIV 620 GCCGGC G^CCGGC NruI 66 TCGCGA TCG^CGA NsiI 4342 ATGCAT ATGCA^T PflMI 549 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3424 CACGTG CAC^GTG PmlI 3424 CACGTG CAC^GTG PshAI 2120 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 828 TCATGA T^CATGA SacI 2309 GAGCTC GAGCT^C SexAI 104 ACCTGGT A^CC[AT]GGT SspBI 1448 TGTACA T^GTACA StuI 790 AGGCCT AGG^CCT SwaI 3587 ATTTAAAT ATTT^AAAT Van91I 549 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3518 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflII 254 CTTAAG C^TTAAG 802 CTTAAG Alw44I 2014 GTGCAC G^TGCAC 4286 GTGCAC AlwNI 762 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 3713 CAGTTACTG Aor51HI 1453 AGCGCT AGC^GCT 2058 AGCGCT ApaLI 2014 GTGCAC G^TGCAC 4286 GTGCAC AseI 1847 ATTAAT AT^TAAT 3224 ATTAAT AsnI 1847 ATTAAT AT^TAAT 3224 ATTAAT Asp700I 1218 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3510 GAACGCCTTC BamHI 1381 GGATCC G^GATCC 4009 GGATCC BanII 2309 GAGCTC G[GA]GC[CT]^C 4144 GGGCCC BfrI 254 CTTAAG C^TTAAG 802 CTTAAG BspMI 1477 ACCTGC ACCTGC(4/8) 2540 GCAGGT BsrBI 33 GAGCGG CCGCTC(-3/-3) 1565 GAGCGG BsrDI 1332 CATTGC GCAATG(2/0) 2826 GCAATG BsrFI 620 GCCGGC [GA]^CCGG[CT] 1776 GCCGGT BstEII 1297 GGTGACC G^GT[ACGT]ACC 3906 GGTAACC BstPI 1297 GGTGACC G^GT[ACGT]ACC 3906 GGTAACC Cfr10I 620 GCCGGC [GA]^CCGG[CT] 1776 GCCGGT EaeI 1699 CGGCCA [CT]^GGCC[GA] 4349 TGGCCA Eco47III 1453 AGCGCT AGC^GCT 2058 AGCGCT EcoO65I 1297 GGTGACC G^GT[ACGT]ACC 3906 GGTAACC EcoRI 2519 GAATTC G^AATTC 3873 GAATTC FauI 1567 GCGGG CCCGC(4/6) 3732 GCGGG HpaI 3846 GTTAAC GTT^AAC 4315 GTTAAC NcoI 1246 CCATGG C^CATGG 3256 CCATGG PshBI 1847 ATTAAT AT^TAAT 3224 ATTAAT ScaI 3020 AGTACT AGT^ACT 3743 AGTACT XmnI 1218 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3510 GAACGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BglII BmyI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I ClaI DdeI DpnII DraI DraII DraIII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII