################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AflII AgeI AscI AviII AvrII BamHI BbrPI BbvCI BcgI BfrI BlnI BsaAI BsiWI BspDI BspMI BsrFI Bst1107I BstZ17I Cfr10I ClaI CpoI DraIII DrdI FseI FspI HpaI KspI MluI NaeI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacII SalI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI SwaI XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 390 GACGTC GACGT^C Acc65I 2324 GGTACC G^GTACC AccI 4390 GTCTAC GT^[AC][GT]AC AccII 1358 CGCG CG^CG AclI 3667 AACGTT AA^CGTT AflIII 3649 ACATGT A^C[GA][CT]GT AhdI 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1203 CCTGAGG CC^T[ACGT]AGG Aor51HI 1884 AGCGCT AGC^GCT ApaI 4023 GGGCCC GGGCC^C Asp718I 2324 GGTACC G^GTACC AspEI 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 135 CTCGAG C^[CT]CG[GA]G BbeI 3620 GGCGCC GGCGC^C BciVI 1851 GGATAC GTATCC(6/5) BclI 4691 TGATCA T^GATCA BglI 4280 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 3585 CGGCCG CG[GA][CT]^CG BsoBI 135 CTCGAG C^[CT]CG[GA]G BspLU11I 3649 ACATGT A^CATGT BsrBI 3588 CCGCTC CCGCTC(-3/-3) BssHII 1357 GCGCGC G^CGCGC BstBI 2269 TTCGAA TT^CGAA BstUI 1358 CGCG CG^CG Bsu36I 1203 CCTGAGG CC^T[ACGT]AGG DraI 3699 TTTAAA TTT^AAA DraII 4023 GGGCCCC [GA]G^G[ACGT]CC[CT] EagI 3585 CGGCCG C^GGCCG Eam1105I 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3585 CGGCCG C^GGCCG Eco47III 1884 AGCGCT AGC^GCT Eco52I 3585 CGGCCG C^GGCCG Eco81I 1203 CCTGAGG CC^T[ACGT]AGG EcoNI 2730 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 4023 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoT22I 3145 ATGCAT ATGCA^T FbaI 4691 TGATCA T^GATCA KasI 3620 GGCGCC G^GCGCC KpnI 2324 GGTACC GGTAC^C MfeI 2312 CAATTG C^AATTG MunI 2312 CAATTG C^AATTG MvnI 1358 CGCG CG^CG NarI 3620 GGCGCC GG^CGCC NsiI 3145 ATGCAT ATGCA^T NspV 2269 TTCGAA TT^CGAA PaeR7I 135 CTCGAG C^TCGAG PflFI 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 3667 AACGTT AA^CGTT SacI 2317 GAGCTC GAGCT^C SapI 2265 GCTCTTC GCTCTTC(1/4) SbfI 404 CCTGCAGG CCTGCA^GG ScaI 1786 AGTACT AGT^ACT SfoI 3620 GGCGCC GGC^GCC SfuI 2269 TTCGAA TT^CGAA Sse8387I 404 CCTGCAGG CCTGCA^GG SspI 4470 AATATT AAT^ATT StuI 4242 AGGCCT AGG^CCT Tth111I 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 135 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 241 TCCGGA T^CCGGA 853 TCCGGA AseI 468 ATTAAT AT^TAAT 4642 ATTAAT AsnI 468 ATTAAT AT^TAAT 4642 ATTAAT Asp700I 2371 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4676 GAATCTTTTC BalI 1627 TGGCCA TGG^CCA 4256 TGGCCA BglII 1660 AGATCT A^GATCT 2386 AGATCT BlpI 1880 GCTTAGC GC^T[ACGT]AGC 2697 GCTCAGC Bpu1102I 1880 GCTTAGC GC^T[ACGT]AGC 2697 GCTCAGC BsaBI 233 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2089 GATGAAGATC BsaI 2213 GAGACC GGTCTC(1/5) 4170 GGTCTC BsaWI 241 TCCGGA [AT]^CCGG[AT] 853 TCCGGA BseAI 241 TCCGGA T^CCGGA 853 TCCGGA BspEI 241 TCCGGA T^CCGGA 853 TCCGGA BspHI 2258 TCATGA T^CATGA 4335 TCATGA BssSI 1913 CACGAG CACGAG(-5/-1) 192 CTCGTG BstEII 1208 GGTTACC G^GT[ACGT]ACC 3598 GGTCACC BstPI 1208 GGTTACC G^GT[ACGT]ACC 3598 GGTCACC BstXI 1426 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3407 CCAGGGGTATGG CelII 1880 GCTTAGC GC^T[ACGT]AGC 2697 GCTCAGC EcoO65I 1208 GGTTACC G^GT[ACGT]ACC 3598 GGTCACC EcoRI 90 GAATTC G^AATTC 3264 GAATTC EcoRV 237 GATATC GAT^ATC 546 GATATC HaeII 1884 AGCGCT [GA]GCGC^[CT] 3620 GGCGCC MamI 233 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2089 GATGAAGATC MluNI 1627 TGGCCA TGG^CCA 4256 TGGCCA MroI 241 TCCGGA T^CCGGA 853 TCCGGA MscI 1627 TGGCCA TGG^CCA 4256 TGGCCA PshBI 468 ATTAAT AT^TAAT 4642 ATTAAT RcaI 2258 TCATGA T^CATGA 4335 TCATGA XmnI 2371 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4676 GAATCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII