##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AflII       AgeI        AscI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BfrI        
BlnI        BsaAI       BsiWI       BspDI       BspMI       
BsrFI       Bst1107I    BstZ17I     Cfr10I      ClaI        
CpoI        DraIII      DrdI        FseI        FspI        
HpaI        KspI        MluI        NaeI        NgoMIV      
NheI        NotI        NruI        PacI        PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PvuI        RsrII       SacII       SalI        SexAI       
SfiI        SgrAI       SmaI        SnaBI       SpeI        
SphI        SwaI        XbaI        XcmI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AatII         390  GACGTC               GACGT^C
Acc65I       2324  GGTACC               G^GTACC
AccI         4390  GTCTAC               GT^[AC][GT]AC
AccII        1358  CGCG                 CG^CG
AclI         3667  AACGTT               AA^CGTT
AflIII       3649  ACATGT               A^C[GA][CT]GT
AhdI         1314  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1203  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1884  AGCGCT               AGC^GCT
ApaI         4023  GGGCCC               GGGCC^C
Asp718I      2324  GGTACC               G^GTACC
AspEI        1314  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          363  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          135  CTCGAG               C^[CT]CG[GA]G
BbeI         3620  GGCGCC               GGCGC^C
BciVI        1851  GGATAC               GTATCC(6/5)
BclI         4691  TGATCA               T^GATCA
BglI         4280  GCCTTTGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI        3585  CGGCCG               CG[GA][CT]^CG
BsoBI         135  CTCGAG               C^[CT]CG[GA]G
BspLU11I     3649  ACATGT               A^CATGT
BsrBI        3588  CCGCTC               CCGCTC(-3/-3)
BssHII       1357  GCGCGC               G^CGCGC
BstBI        2269  TTCGAA               TT^CGAA
BstUI        1358  CGCG                 CG^CG
Bsu36I       1203  CCTGAGG              CC^T[ACGT]AGG
DraI         3699  TTTAAA               TTT^AAA
DraII        4023  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EagI         3585  CGGCCG               C^GGCCG
Eam1105I     1314  GACCAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        3585  CGGCCG               C^GGCCG
Eco47III     1884  AGCGCT               AGC^GCT
Eco52I       3585  CGGCCG               C^GGCCG
Eco81I       1203  CCTGAGG              CC^T[ACGT]AGG
EcoNI        2730  CCTGACCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     4023  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      3145  ATGCAT               ATGCA^T
FbaI         4691  TGATCA               T^GATCA
KasI         3620  GGCGCC               G^GCGCC
KpnI         2324  GGTACC               GGTAC^C
MfeI         2312  CAATTG               C^AATTG
MunI         2312  CAATTG               C^AATTG
MvnI         1358  CGCG                 CG^CG
NarI         3620  GGCGCC               GG^CGCC
NsiI         3145  ATGCAT               ATGCA^T
NspV         2269  TTCGAA               TT^CGAA
PaeR7I        135  CTCGAG               C^TCGAG
PflFI         363  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I     3667  AACGTT               AA^CGTT
SacI         2317  GAGCTC               GAGCT^C
SapI         2265  GCTCTTC              GCTCTTC(1/4)
SbfI          404  CCTGCAGG             CCTGCA^GG
ScaI         1786  AGTACT               AGT^ACT
SfoI         3620  GGCGCC               GGC^GCC
SfuI         2269  TTCGAA               TT^CGAA
Sse8387I      404  CCTGCAGG             CCTGCA^GG
SspI         4470  AATATT               AAT^ATT
StuI         4242  AGGCCT               AGG^CCT
Tth111I       363  GACTATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          135  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        241  TCCGGA               T^CCGGA
              853  TCCGGA              
AseI          468  ATTAAT               AT^TAAT
             4642  ATTAAT              
AsnI          468  ATTAAT               AT^TAAT
             4642  ATTAAT              
Asp700I      2371  GAATTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4676  GAATCTTTTC          
BalI         1627  TGGCCA               TGG^CCA
             4256  TGGCCA              
BglII        1660  AGATCT               A^GATCT
             2386  AGATCT              
BlpI         1880  GCTTAGC              GC^T[ACGT]AGC
             2697  GCTCAGC             
Bpu1102I     1880  GCTTAGC              GC^T[ACGT]AGC
             2697  GCTCAGC             
BsaBI         233  GATAGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2089  GATGAAGATC          
BsaI         2213  GAGACC               GGTCTC(1/5)
             4170  GGTCTC              
BsaWI         241  TCCGGA               [AT]^CCGG[AT]
              853  TCCGGA              
BseAI         241  TCCGGA               T^CCGGA
              853  TCCGGA              
BspEI         241  TCCGGA               T^CCGGA
              853  TCCGGA              
BspHI        2258  TCATGA               T^CATGA
             4335  TCATGA              
BssSI        1913  CACGAG               CACGAG(-5/-1)
              192  CTCGTG              
BstEII       1208  GGTTACC              G^GT[ACGT]ACC
             3598  GGTCACC             
BstPI        1208  GGTTACC              G^GT[ACGT]ACC
             3598  GGTCACC             
BstXI        1426  CCAGCCCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3407  CCAGGGGTATGG        
CelII        1880  GCTTAGC              GC^T[ACGT]AGC
             2697  GCTCAGC             
EcoO65I      1208  GGTTACC              G^GT[ACGT]ACC
             3598  GGTCACC             
EcoRI          90  GAATTC               G^AATTC
             3264  GAATTC              
EcoRV         237  GATATC               GAT^ATC
              546  GATATC              
HaeII        1884  AGCGCT               [GA]GCGC^[CT]
             3620  GGCGCC              
MamI          233  GATAGATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2089  GATGAAGATC          
MluNI        1627  TGGCCA               TGG^CCA
             4256  TGGCCA              
MroI          241  TCCGGA               T^CCGGA
              853  TCCGGA              
MscI         1627  TGGCCA               TGG^CCA
             4256  TGGCCA              
PshBI         468  ATTAAT               AT^TAAT
             4642  ATTAAT              
RcaI         2258  TCATGA               T^CATGA
             4335  TCATGA              
XmnI         2371  GAATTAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4676  GAATCTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       ApaLI       ApoI        
AspHI       AvaII       BanI        BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BsrDI       
BsrGI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EarI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NcoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XhoII