##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AflII AgeI AscI AviII AvrII
BamHI BbrPI BbvCI BcgI BfrI
BlnI BsaAI BsiWI BspDI BspMI
BsrFI Bst1107I BstZ17I Cfr10I ClaI
CpoI DraIII DrdI FseI FspI
HpaI KspI MluI NaeI NgoMIV
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PvuI RsrII SacII SalI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI SwaI XbaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 390 GACGTC GACGT^C
Acc65I 2324 GGTACC G^GTACC
AccI 4390 GTCTAC GT^[AC][GT]AC
AccII 1358 CGCG CG^CG
AclI 3667 AACGTT AA^CGTT
AflIII 3649 ACATGT A^C[GA][CT]GT
AhdI 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1203 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1884 AGCGCT AGC^GCT
ApaI 4023 GGGCCC GGGCC^C
Asp718I 2324 GGTACC G^GTACC
AspEI 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 135 CTCGAG C^[CT]CG[GA]G
BbeI 3620 GGCGCC GGCGC^C
BciVI 1851 GGATAC GTATCC(6/5)
BclI 4691 TGATCA T^GATCA
BglI 4280 GCCTTTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 3585 CGGCCG CG[GA][CT]^CG
BsoBI 135 CTCGAG C^[CT]CG[GA]G
BspLU11I 3649 ACATGT A^CATGT
BsrBI 3588 CCGCTC CCGCTC(-3/-3)
BssHII 1357 GCGCGC G^CGCGC
BstBI 2269 TTCGAA TT^CGAA
BstUI 1358 CGCG CG^CG
Bsu36I 1203 CCTGAGG CC^T[ACGT]AGG
DraI 3699 TTTAAA TTT^AAA
DraII 4023 GGGCCCC [GA]G^G[ACGT]CC[CT]
EagI 3585 CGGCCG C^GGCCG
Eam1105I 1314 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3585 CGGCCG C^GGCCG
Eco47III 1884 AGCGCT AGC^GCT
Eco52I 3585 CGGCCG C^GGCCG
Eco81I 1203 CCTGAGG CC^T[ACGT]AGG
EcoNI 2730 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 4023 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 3145 ATGCAT ATGCA^T
FbaI 4691 TGATCA T^GATCA
KasI 3620 GGCGCC G^GCGCC
KpnI 2324 GGTACC GGTAC^C
MfeI 2312 CAATTG C^AATTG
MunI 2312 CAATTG C^AATTG
MvnI 1358 CGCG CG^CG
NarI 3620 GGCGCC GG^CGCC
NsiI 3145 ATGCAT ATGCA^T
NspV 2269 TTCGAA TT^CGAA
PaeR7I 135 CTCGAG C^TCGAG
PflFI 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3667 AACGTT AA^CGTT
SacI 2317 GAGCTC GAGCT^C
SapI 2265 GCTCTTC GCTCTTC(1/4)
SbfI 404 CCTGCAGG CCTGCA^GG
ScaI 1786 AGTACT AGT^ACT
SfoI 3620 GGCGCC GGC^GCC
SfuI 2269 TTCGAA TT^CGAA
Sse8387I 404 CCTGCAGG CCTGCA^GG
SspI 4470 AATATT AAT^ATT
StuI 4242 AGGCCT AGG^CCT
Tth111I 363 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 135 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 241 TCCGGA T^CCGGA
853 TCCGGA
AseI 468 ATTAAT AT^TAAT
4642 ATTAAT
AsnI 468 ATTAAT AT^TAAT
4642 ATTAAT
Asp700I 2371 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4676 GAATCTTTTC
BalI 1627 TGGCCA TGG^CCA
4256 TGGCCA
BglII 1660 AGATCT A^GATCT
2386 AGATCT
BlpI 1880 GCTTAGC GC^T[ACGT]AGC
2697 GCTCAGC
Bpu1102I 1880 GCTTAGC GC^T[ACGT]AGC
2697 GCTCAGC
BsaBI 233 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2089 GATGAAGATC
BsaI 2213 GAGACC GGTCTC(1/5)
4170 GGTCTC
BsaWI 241 TCCGGA [AT]^CCGG[AT]
853 TCCGGA
BseAI 241 TCCGGA T^CCGGA
853 TCCGGA
BspEI 241 TCCGGA T^CCGGA
853 TCCGGA
BspHI 2258 TCATGA T^CATGA
4335 TCATGA
BssSI 1913 CACGAG CACGAG(-5/-1)
192 CTCGTG
BstEII 1208 GGTTACC G^GT[ACGT]ACC
3598 GGTCACC
BstPI 1208 GGTTACC G^GT[ACGT]ACC
3598 GGTCACC
BstXI 1426 CCAGCCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3407 CCAGGGGTATGG
CelII 1880 GCTTAGC GC^T[ACGT]AGC
2697 GCTCAGC
EcoO65I 1208 GGTTACC G^GT[ACGT]ACC
3598 GGTCACC
EcoRI 90 GAATTC G^AATTC
3264 GAATTC
EcoRV 237 GATATC GAT^ATC
546 GATATC
HaeII 1884 AGCGCT [GA]GCGC^[CT]
3620 GGCGCC
MamI 233 GATAGATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2089 GATGAAGATC
MluNI 1627 TGGCCA TGG^CCA
4256 TGGCCA
MroI 241 TCCGGA T^CCGGA
853 TCCGGA
MscI 1627 TGGCCA TGG^CCA
4256 TGGCCA
PshBI 468 ATTAAT AT^TAAT
4642 ATTAAT
RcaI 2258 TCATGA T^CATGA
4335 TCATGA
XmnI 2371 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4676 GAATCTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII