##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AscI AseI AsnI Asp700I Asp718I
AviII BciVI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MfeI MluI MunI
NdeI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 3387 GTAGAC GT^[AC][GT]AC
AccIII 1308 TCCGGA T^CCGGA
AcsI 4767 AAATTC [GA]^AATT[CT]
AflII 2812 CTTAAG C^TTAAG
AhdI 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4161 GTGCAC G^TGCAC
ApaLI 4161 GTGCAC G^TGCAC
ApoI 4767 AAATTC [GA]^AATT[CT]
AspEI 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1130 CCTAGG C^CTAGG
BamHI 937 GGATCC G^GATCC
BbrPI 4025 CACGTG CAC^GTG
BbvCI 4624 GCTGAGG CCTCAGC(-5/-2)
BclI 517 TGATCA T^GATCA
BfrI 2812 CTTAAG C^TTAAG
BglII 1010 AGATCT A^GATCT
BlnI 1130 CCTAGG C^CTAGG
BsaAI 4025 CACGTG [CT]AC^GT[GA]
BsaBI 1376 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1308 TCCGGA [AT]^CCGG[AT]
BseAI 1308 TCCGGA T^CCGGA
BsmBI 3743 CGTCTC CGTCTC(1/5)
BspEI 1308 TCCGGA T^CCGGA
BspHI 4596 TCATGA T^CATGA
BssHII 6 GCGCGC G^CGCGC
BstAPI 2377 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 4155 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2554 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 517 TGATCA T^GATCA
HgaI 90 GACGC GACGC(5/10)
MamI 1376 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1308 TCCGGA T^CCGGA
NcoI 933 CCATGG C^CATGG
NheI 2477 GCTAGC G^CTAGC
PflFI 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4025 CACGTG CAC^GTG
PmlI 4025 CACGTG CAC^GTG
PshAI 3009 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4596 TCATGA T^CATGA
SexAI 3759 ACCTGGT A^CC[AT]GGT
SspI 2171 AATATT AAT^ATT
Tsp509I 4768 AATT ^AATT
Tth111I 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 7 CGCG CG^CG
92 CGCG
AcyI 1369 GGCGCC G[GA]^CG[CT]C
3696 GGCGCC
Aor51HI 2399 AGCGCT AGC^GCT
2745 AGCGCT
BbeI 1369 GGCGCC GGCGC^C
3696 GGCGCC
BcgI 3253 CGAATACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
373 GCAGGACCATCG
BglI 1350 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1557 GCCTAGTGGGC
BsaHI 1369 GGCGCC G[GA]^CG[CT]C
3696 GGCGCC
BstEII 1930 GGTGACC G^GT[ACGT]ACC
2333 GGTGACC
BstPI 1930 GGTGACC G^GT[ACGT]ACC
2333 GGTGACC
BstUI 7 CGCG CG^CG
92 CGCG
Eco47III 2399 AGCGCT AGC^GCT
2745 AGCGCT
EcoO65I 1930 GGTGACC G^GT[ACGT]ACC
2333 GGTGACC
Hin1I 1369 GGCGCC G[GA]^CG[CT]C
3696 GGCGCC
HincII 3025 GTTGAC GT[CT]^[GA]AC
4205 GTCAAC
HindII 3025 GTTGAC GT[CT]^[GA]AC
4205 GTCAAC
HindIII 3411 AAGCTT A^AGCTT
4331 AAGCTT
KasI 1369 GGCGCC G^GCGCC
3696 GGCGCC
MseI 111 TTAA T^TAA
2813 TTAA
MvnI 7 CGCG CG^CG
92 CGCG
NarI 1369 GGCGCC GG^CGCC
3696 GGCGCC
PflMI 3058 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3564 CCACTGACTGG
SapI 1097 GAAGAGC GCTCTTC(1/4)
1664 GCTCTTC
ScaI 183 AGTACT AGT^ACT
3048 AGTACT
SfoI 1369 GGCGCC GGC^GCC
3696 GGCGCC
StuI 2289 AGGCCT AGG^CCT
2691 AGGCCT
Tru9I 111 TTAA T^TAA
2813 TTAA
Van91I 3058 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3564 CCACTGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII