################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI AflIII AgeI AscI AseI AsnI Asp700I Asp718I AviII BciVI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI KspI MfeI MluI MunI NdeI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 3387 GTAGAC GT^[AC][GT]AC AccIII 1308 TCCGGA T^CCGGA AcsI 4767 AAATTC [GA]^AATT[CT] AflII 2812 CTTAAG C^TTAAG AhdI 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4161 GTGCAC G^TGCAC ApaLI 4161 GTGCAC G^TGCAC ApoI 4767 AAATTC [GA]^AATT[CT] AspEI 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1130 CCTAGG C^CTAGG BamHI 937 GGATCC G^GATCC BbrPI 4025 CACGTG CAC^GTG BbvCI 4624 GCTGAGG CCTCAGC(-5/-2) BclI 517 TGATCA T^GATCA BfrI 2812 CTTAAG C^TTAAG BglII 1010 AGATCT A^GATCT BlnI 1130 CCTAGG C^CTAGG BsaAI 4025 CACGTG [CT]AC^GT[GA] BsaBI 1376 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1308 TCCGGA [AT]^CCGG[AT] BseAI 1308 TCCGGA T^CCGGA BsmBI 3743 CGTCTC CGTCTC(1/5) BspEI 1308 TCCGGA T^CCGGA BspHI 4596 TCATGA T^CATGA BssHII 6 GCGCGC G^CGCGC BstAPI 2377 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 4155 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2554 GACACAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1866 GACATGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 517 TGATCA T^GATCA HgaI 90 GACGC GACGC(5/10) MamI 1376 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1308 TCCGGA T^CCGGA NcoI 933 CCATGG C^CATGG NheI 2477 GCTAGC G^CTAGC PflFI 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4025 CACGTG CAC^GTG PmlI 4025 CACGTG CAC^GTG PshAI 3009 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4596 TCATGA T^CATGA SexAI 3759 ACCTGGT A^CC[AT]GGT SspI 2171 AATATT AAT^ATT Tsp509I 4768 AATT ^AATT Tth111I 1637 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 7 CGCG CG^CG 92 CGCG AcyI 1369 GGCGCC G[GA]^CG[CT]C 3696 GGCGCC Aor51HI 2399 AGCGCT AGC^GCT 2745 AGCGCT BbeI 1369 GGCGCC GGCGC^C 3696 GGCGCC BcgI 3253 CGAATACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 373 GCAGGACCATCG BglI 1350 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1557 GCCTAGTGGGC BsaHI 1369 GGCGCC G[GA]^CG[CT]C 3696 GGCGCC BstEII 1930 GGTGACC G^GT[ACGT]ACC 2333 GGTGACC BstPI 1930 GGTGACC G^GT[ACGT]ACC 2333 GGTGACC BstUI 7 CGCG CG^CG 92 CGCG Eco47III 2399 AGCGCT AGC^GCT 2745 AGCGCT EcoO65I 1930 GGTGACC G^GT[ACGT]ACC 2333 GGTGACC Hin1I 1369 GGCGCC G[GA]^CG[CT]C 3696 GGCGCC HincII 3025 GTTGAC GT[CT]^[GA]AC 4205 GTCAAC HindII 3025 GTTGAC GT[CT]^[GA]AC 4205 GTCAAC HindIII 3411 AAGCTT A^AGCTT 4331 AAGCTT KasI 1369 GGCGCC G^GCGCC 3696 GGCGCC MseI 111 TTAA T^TAA 2813 TTAA MvnI 7 CGCG CG^CG 92 CGCG NarI 1369 GGCGCC GG^CGCC 3696 GGCGCC PflMI 3058 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3564 CCACTGACTGG SapI 1097 GAAGAGC GCTCTTC(1/4) 1664 GCTCTTC ScaI 183 AGTACT AGT^ACT 3048 AGTACT SfoI 1369 GGCGCC GGC^GCC 3696 GGCGCC StuI 2289 AGGCCT AGG^CCT 2691 AGGCCT Tru9I 111 TTAA T^TAA 2813 TTAA Van91I 3058 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3564 CCACTGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII