################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BfrI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmI BspEI Bst1107I BstBI BstZ17I CelII CpoI DrdI Eam1105I Eco47III FseI HpaI KpnI MamI MfeI MluI MroI MunI NheI NruI NspV PacI PaeR7I PflFI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AcyI 78 GGCGCC G[GA]^CG[CT]C AgeI 1409 ACCGGT A^CCGGT AocI 2289 CCTGAGG CC^T[ACGT]AGG ApaI 2320 GGGCCC GGGCC^C AviII 439 TGCGCA TGC^GCA BbeI 78 GGCGCC GGCGC^C BbrPI 600 CACGTG CAC^GTG BcgI 361 CGACATCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 3059 AGATCT A^GATCT BsaAI 600 CACGTG [CT]AC^GT[GA] BsaHI 78 GGCGCC G[GA]^CG[CT]C BspDI 2988 ATCGAT AT^CGAT BspHI 2419 TCATGA T^CATGA BspLU11I 1581 ACATGT A^CATGT BsrBI 1506 GAGCGG CCGCTC(-3/-3) BssHII 39 GCGCGC G^CGCGC BssSI 3550 CTCGTG CACGAG(-5/-1) BstXI 2031 CCACTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2289 CCTGAGG CC^T[ACGT]AGG ClaI 2988 ATCGAT AT^CGAT Eco81I 2289 CCTGAGG CC^T[ACGT]AGG EcoRI 1078 GAATTC G^AATTC EcoRV 1236 GATATC GAT^ATC EcoT22I 1161 ATGCAT ATGCA^T FspI 439 TGCGCA TGC^GCA Hin1I 78 GGCGCC G[GA]^CG[CT]C HincII 2019 GTTGAC GT[CT]^[GA]AC HindII 2019 GTTGAC GT[CT]^[GA]AC HindIII 2836 AAGCTT A^AGCTT KasI 78 GGCGCC G^GCGCC KspI 260 CCGCGG CCGC^GG NarI 78 GGCGCC GG^CGCC NotI 283 GCGGCCGC GC^GGCCGC NsiI 1161 ATGCAT ATGCA^T PinAI 1409 ACCGGT A^CCGGT PmaCI 600 CACGTG CAC^GTG PmeI 3706 GTTTAAAC GTTT^AAAC PmlI 600 CACGTG CAC^GTG PpuMI 2569 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 2419 TCATGA T^CATGA SacII 260 CCGCGG CCGC^GG SapI 2255 GCTCTTC GCTCTTC(1/4) ScaI 2515 AGTACT AGT^ACT SexAI 106 ACCAGGT A^CC[AT]GGT SfiI 276 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 78 GGCGCC GGC^GCC SphI 1939 GCATGC GCATG^C XbaI 4112 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 1581 ACATGT A^C[GA][CT]GT 2786 ACACGT Alw44I 597 GTGCAC G^TGCAC 4398 GTGCAC ApaLI 597 GTGCAC G^TGCAC 4398 GTGCAC BbvCI 908 CCTCAGC CCTCAGC(-5/-2) 3793 GCTGAGG BciVI 1863 GGATAC GTATCC(6/5) 2775 GGATAC BclI 643 TGATCA T^GATCA 2090 TGATCA BglI 176 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 277 GCCTCGGCGGC BsaWI 14 TCCGGT [AT]^CCGG[AT] 1409 ACCGGT BsmBI 2328 GAGACG CGTCTC(1/5) 3589 GAGACG DraI 3707 TTTAAA TTT^AAA 3872 TTTAAA EarI 2256 CTCTTC CTCTTC(1/4) 548 GAAGAG EcoNI 101 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1466 CCTTGAGAAGG FbaI 643 TGATCA T^GATCA 2090 TGATCA HaeII 78 GGCGCC [GA]GCGC^[CT] 2342 GGCGCT Ksp632I 2256 CTCTTC CTCTTC(1/4) 548 GAAGAG NaeI 53 GCCGGC GCC^GGC 1386 GCCGGC NdeI 2119 CATATG CA^TATG 3843 CATATG NgoMIV 53 GCCGGC G^CCGGC 1386 GCCGGC SacI 2415 GAGCTC GAGCT^C 3231 GAGCTC SmaI 272 CCCGGG CCC^GGG 2594 CCCGGG XcmI 1212 CCATTGAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3435 CCAGCACCAGCCTGG XmaCI 272 CCCGGG C^CCGGG 2594 CCCGGG XmaI 272 CCCGGG C^CCGGG 2594 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII