##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmI BspEI
Bst1107I BstBI BstZ17I CelII CpoI
DrdI Eam1105I Eco47III FseI HpaI
KpnI MamI MfeI MluI MroI
MunI NheI NruI NspV PacI
PaeR7I PflFI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 78 GGCGCC G[GA]^CG[CT]C
AgeI 1409 ACCGGT A^CCGGT
AocI 2289 CCTGAGG CC^T[ACGT]AGG
ApaI 2320 GGGCCC GGGCC^C
AviII 439 TGCGCA TGC^GCA
BbeI 78 GGCGCC GGCGC^C
BbrPI 600 CACGTG CAC^GTG
BcgI 361 CGACATCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3059 AGATCT A^GATCT
BsaAI 600 CACGTG [CT]AC^GT[GA]
BsaHI 78 GGCGCC G[GA]^CG[CT]C
BspDI 2988 ATCGAT AT^CGAT
BspHI 2419 TCATGA T^CATGA
BspLU11I 1581 ACATGT A^CATGT
BsrBI 1506 GAGCGG CCGCTC(-3/-3)
BssHII 39 GCGCGC G^CGCGC
BssSI 3550 CTCGTG CACGAG(-5/-1)
BstXI 2031 CCACTGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2289 CCTGAGG CC^T[ACGT]AGG
ClaI 2988 ATCGAT AT^CGAT
Eco81I 2289 CCTGAGG CC^T[ACGT]AGG
EcoRI 1078 GAATTC G^AATTC
EcoRV 1236 GATATC GAT^ATC
EcoT22I 1161 ATGCAT ATGCA^T
FspI 439 TGCGCA TGC^GCA
Hin1I 78 GGCGCC G[GA]^CG[CT]C
HincII 2019 GTTGAC GT[CT]^[GA]AC
HindII 2019 GTTGAC GT[CT]^[GA]AC
HindIII 2836 AAGCTT A^AGCTT
KasI 78 GGCGCC G^GCGCC
KspI 260 CCGCGG CCGC^GG
NarI 78 GGCGCC GG^CGCC
NotI 283 GCGGCCGC GC^GGCCGC
NsiI 1161 ATGCAT ATGCA^T
PinAI 1409 ACCGGT A^CCGGT
PmaCI 600 CACGTG CAC^GTG
PmeI 3706 GTTTAAAC GTTT^AAAC
PmlI 600 CACGTG CAC^GTG
PpuMI 2569 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 2419 TCATGA T^CATGA
SacII 260 CCGCGG CCGC^GG
SapI 2255 GCTCTTC GCTCTTC(1/4)
ScaI 2515 AGTACT AGT^ACT
SexAI 106 ACCAGGT A^CC[AT]GGT
SfiI 276 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 78 GGCGCC GGC^GCC
SphI 1939 GCATGC GCATG^C
XbaI 4112 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1581 ACATGT A^C[GA][CT]GT
2786 ACACGT
Alw44I 597 GTGCAC G^TGCAC
4398 GTGCAC
ApaLI 597 GTGCAC G^TGCAC
4398 GTGCAC
BbvCI 908 CCTCAGC CCTCAGC(-5/-2)
3793 GCTGAGG
BciVI 1863 GGATAC GTATCC(6/5)
2775 GGATAC
BclI 643 TGATCA T^GATCA
2090 TGATCA
BglI 176 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
277 GCCTCGGCGGC
BsaWI 14 TCCGGT [AT]^CCGG[AT]
1409 ACCGGT
BsmBI 2328 GAGACG CGTCTC(1/5)
3589 GAGACG
DraI 3707 TTTAAA TTT^AAA
3872 TTTAAA
EarI 2256 CTCTTC CTCTTC(1/4)
548 GAAGAG
EcoNI 101 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1466 CCTTGAGAAGG
FbaI 643 TGATCA T^GATCA
2090 TGATCA
HaeII 78 GGCGCC [GA]GCGC^[CT]
2342 GGCGCT
Ksp632I 2256 CTCTTC CTCTTC(1/4)
548 GAAGAG
NaeI 53 GCCGGC GCC^GGC
1386 GCCGGC
NdeI 2119 CATATG CA^TATG
3843 CATATG
NgoMIV 53 GCCGGC G^CCGGC
1386 GCCGGC
SacI 2415 GAGCTC GAGCT^C
3231 GAGCTC
SmaI 272 CCCGGG CCC^GGG
2594 CCCGGG
XcmI 1212 CCATTGAAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3435 CCAGCACCAGCCTGG
XmaCI 272 CCCGGG C^CCGGG
2594 CCCGGG
XmaI 272 CCCGGG C^CCGGG
2594 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII