##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AocI ApaI AscI Asp700I Asp718I
AspEI BamHI BbvCI BfrI BsaBI
BsiWI Bsp1407I BspDI BspLU11I BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI DraIII Eam1105I Eco81I
EcoO65I EcoT22I FseI KpnI MamI
MfeI MluI MunI NcoI NdeI
NheI NsiI NspV PacI PaeR7I
PmeI Psp1406I SbfI ScaI SexAI
SfiI SfuI SgrAI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AgeI 4165 ACCGGT A^CCGGT
Alw44I 1410 GTGCAC G^TGCAC
Aor51HI 3814 AGCGCT AGC^GCT
ApaLI 1410 GTGCAC G^TGCAC
AseI 550 ATTAAT AT^TAAT
AsnI 550 ATTAAT AT^TAAT
AspI 864 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1841 TGCGCA TGC^GCA
AvrII 441 CCTAGG C^CTAGG
BbeI 2549 GGCGCC GGCGC^C
BcgI 1354 GCAACCGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4440 GTATCC GTATCC(6/5)
BclI 3587 TGATCA T^GATCA
BglII 1213 AGATCT A^GATCT
BlnI 441 CCTAGG C^CTAGG
BsaI 1029 GAGACC GGTCTC(1/5)
BsmI 3519 GCATTC GAATGC(1/-1)
BspHI 3590 TCATGA T^CATGA
BstAPI 1403 GCAAGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 2287 CGGACCG CG^G[AT]CCG
DraI 4322 TTTAAA TTT^AAA
DrdI 3771 GACACCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3814 AGCGCT AGC^GCT
EcoNI 1653 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1607 GAATTC G^AATTC
FbaI 3587 TGATCA T^GATCA
FspI 1841 TGCGCA TGC^GCA
HindIII 4520 AAGCTT A^AGCTT
HpaI 3507 GTTAAC GTT^AAC
KasI 2549 GGCGCC G^GCGCC
KspI 1429 CCGCGG CCGC^GG
NarI 2549 GGCGCC GG^CGCC
NotI 3136 GCGGCCGC GC^GGCCGC
PflFI 864 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2477 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 4165 ACCGGT A^CCGGT
PshBI 550 ATTAAT AT^TAAT
PvuI 1973 CGATCG CGAT^CG
RcaI 3590 TCATGA T^CATGA
RsrII 2287 CGGACCG CG^G[AT]CCG
SacII 1429 CCGCGG CCGC^GG
SalI 3989 GTCGAC G^TCGAC
SfoI 2549 GGCGCC GGC^GCC
SmlI 2037 CTCAAG C^T[CT][GA]AG
SnaBI 1044 TACGTA TAC^GTA
StuI 4115 AGGCCT AGG^CCT
Tth111I 864 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2477 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2223 GTCTAC GT^[AC][GT]AC
3989 GTCGAC
AccIII 212 TCCGGA T^CCGGA
3243 TCCGGA
AlwNI 2478 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
3361 CAGATGCTG
BbrPI 3071 CACGTG CAC^GTG
4088 CACGTG
BseAI 212 TCCGGA T^CCGGA
3243 TCCGGA
BsmBI 639 GAGACG CGTCTC(1/5)
2020 GAGACG
BspEI 212 TCCGGA T^CCGGA
3243 TCCGGA
BsrDI 2491 GCAATG GCAATG(2/0)
3115 GCAATG
BssHII 2745 GCGCGC G^CGCGC
3153 GCGCGC
BssSI 3898 CACGAG CACGAG(-5/-1)
2626 CTCGTG
EagI 1745 CGGCCG C^GGCCG
3137 CGGCCG
EclXI 1745 CGGCCG C^GGCCG
3137 CGGCCG
Eco52I 1745 CGGCCG C^GGCCG
3137 CGGCCG
EcoRV 320 GATATC GAT^ATC
3180 GATATC
MroI 212 TCCGGA T^CCGGA
3243 TCCGGA
NruI 1064 TCGCGA TCG^CGA
3079 TCGCGA
NspI 331 GCATGT [GA]CATG^[CT]
3743 GCATGT
PmaCI 3071 CACGTG CAC^GTG
4088 CACGTG
PmlI 3071 CACGTG CAC^GTG
4088 CACGTG
PvuII 2853 CAGCTG CAG^CTG
3616 CAGCTG
SapI 900 GAAGAGC GCTCTTC(1/4)
1721 GCTCTTC
SmaI 2458 CCCGGG CCC^GGG
3148 CCCGGG
TfiI 664 GAATC G^A[AT]TC
1360 GATTC
XcmI 2833 CCACAGCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3019 CCATGATCGTGTTGG
XmaCI 2458 CCCGGG C^CCGGG
3148 CCCGGG
XmaI 2458 CCCGGG C^CCGGG
3148 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PshAI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII