################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AclI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AvrII BbeI BcgI BglII BlnI BsiWI BspDI BstBI ClaI CpoI DraI Eco47III EcoRI EcoRV FseI HincII HindII HindIII HpaI KasI KspI MluI NarI NheI NotI NruI NspV PacI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI StuI SwaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 674 TCCGGA T^CCGGA AcyI 2841 GACGCC G[GA]^CG[CT]C AflII 2061 CTTAAG C^TTAAG AgeI 1005 ACCGGT A^CCGGT AhdI 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1186 CCTCAGG CC^T[ACGT]AGG Asp700I 4399 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1680 CACGTG CAC^GTG BciVI 389 GGATAC GTATCC(6/5) BclI 3250 TGATCA T^GATCA BfrI 2061 CTTAAG C^TTAAG BlpI 337 GCTCAGC GC^T[ACGT]AGC Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC BsaBI 2117 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2841 GACGCC G[GA]^CG[CT]C BseAI 674 TCCGGA T^CCGGA BsmI 212 GAATGC GAATGC(1/-1) Bsp1407I 3810 TGTACA T^GTACA BspEI 674 TCCGGA T^CCGGA BspHI 3280 TCATGA T^CATGA BsrGI 3810 TGTACA T^GTACA BssHII 3097 GCGCGC G^CGCGC Bst1107I 4262 GTATAC GTA^TAC BstZ17I 4262 GTATAC GTA^TAC Bsu36I 1186 CCTCAGG CC^T[ACGT]AGG CelII 337 GCTCAGC GC^T[ACGT]AGC DraIII 3942 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 698 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2480 CGGCCG C^GGCCG Eam1105I 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2480 CGGCCG C^GGCCG Eco52I 2480 CGGCCG C^GGCCG Eco81I 1186 CCTCAGG CC^T[ACGT]AGG FbaI 3250 TGATCA T^GATCA Hin1I 2841 GACGCC G[GA]^CG[CT]C MamI 2117 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4141 CAATTG C^AATTG MroI 674 TCCGGA T^CCGGA MunI 4141 CAATTG C^AATTG NcoI 4165 CCATGG C^CATGG NdeI 1768 CATATG CA^TATG PflFI 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1005 ACCGGT A^CCGGT PmaCI 1680 CACGTG CAC^GTG PmlI 1680 CACGTG CAC^GTG RcaI 3280 TCATGA T^CATGA SexAI 3342 ACCAGGT A^CC[AT]GGT SmaI 2731 CCCGGG CCC^GGG SspBI 3810 TGTACA T^GTACA SspI 4401 AATATT AAT^ATT Tth111I 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1301 TCTAGA T^CTAGA XmaCI 2731 CCCGGG C^CCGGG XmaI 2731 CCCGGG C^CCGGG XmnI 4399 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 489 GGTACC G^GTACC 2668 GGTACC AcsI 1995 GAATTT [GA]^AATT[CT] 3284 GAATTT ApoI 1995 GAATTT [GA]^AATT[CT] 3284 GAATTT Asp718I 489 GGTACC G^GTACC 2668 GGTACC BalI 3924 TGGCCA TGG^CCA 3938 TGGCCA BamHI 1555 GGATCC G^GATCC 3588 GGATCC BsaAI 1680 CACGTG [CT]AC^GT[GA] 3692 TACGTG BsaWI 674 TCCGGA [AT]^CCGG[AT] 1005 ACCGGT BsiEI 2480 CGGCCG CG[GA][CT]^CG 3219 CGGTCG BspLU11I 1782 ACATGT A^CATGT 1846 ACATGT BsrDI 2085 GCAATG GCAATG(2/0) 2419 GCAATG BssSI 786 CACGAG CACGAG(-5/-1) 3182 CACGAG BstEII 1465 GGTCACC G^GT[ACGT]ACC 2399 GGTCACC BstPI 1465 GGTCACC G^GT[ACGT]ACC 2399 GGTCACC EcoNI 1961 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3484 CCTGAAGCAGG EcoO65I 1465 GGTCACC G^GT[ACGT]ACC 2399 GGTCACC EcoT22I 3996 ATGCAT ATGCA^T 4376 ATGCAT KpnI 489 GGTACC GGTAC^C 2668 GGTACC MaeII 1681 ACGT A^CGT 3693 ACGT MluNI 3924 TGGCCA TGG^CCA 3938 TGGCCA MscI 3924 TGGCCA TGG^CCA 3938 TGGCCA NaeI 78 GCCGGC GCC^GGC 2478 GCCGGC NgoMIV 78 GCCGGC G^CCGGC 2478 GCCGGC NsiI 3996 ATGCAT ATGCA^T 4376 ATGCAT PaeR7I 1240 CTCGAG C^TCGAG 1397 CTCGAG PflMI 2360 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3457 CCAGCACCTGG PshAI 917 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3657 GACAACTGTC SapI 312 GAAGAGC GCTCTTC(1/4) 2424 GAAGAGC Van91I 2360 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3457 CCAGCACCTGG XcmI 280 CCAAGGACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4036 CCATCCTGGTATTGG XhoI 1240 CTCGAG C^TCGAG 1397 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII AviII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI SphI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII