##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AclI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AvrII
BbeI BcgI BglII BlnI BsiWI
BspDI BstBI ClaI CpoI DraI
Eco47III EcoRI EcoRV FseI HincII
HindII HindIII HpaI KasI KspI
MluI NarI NheI NotI NruI
NspV PacI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI StuI SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 674 TCCGGA T^CCGGA
AcyI 2841 GACGCC G[GA]^CG[CT]C
AflII 2061 CTTAAG C^TTAAG
AgeI 1005 ACCGGT A^CCGGT
AhdI 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1186 CCTCAGG CC^T[ACGT]AGG
Asp700I 4399 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1680 CACGTG CAC^GTG
BciVI 389 GGATAC GTATCC(6/5)
BclI 3250 TGATCA T^GATCA
BfrI 2061 CTTAAG C^TTAAG
BlpI 337 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 337 GCTCAGC GC^T[ACGT]AGC
BsaBI 2117 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2841 GACGCC G[GA]^CG[CT]C
BseAI 674 TCCGGA T^CCGGA
BsmI 212 GAATGC GAATGC(1/-1)
Bsp1407I 3810 TGTACA T^GTACA
BspEI 674 TCCGGA T^CCGGA
BspHI 3280 TCATGA T^CATGA
BsrGI 3810 TGTACA T^GTACA
BssHII 3097 GCGCGC G^CGCGC
Bst1107I 4262 GTATAC GTA^TAC
BstZ17I 4262 GTATAC GTA^TAC
Bsu36I 1186 CCTCAGG CC^T[ACGT]AGG
CelII 337 GCTCAGC GC^T[ACGT]AGC
DraIII 3942 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 698 GACAGGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2480 CGGCCG C^GGCCG
Eam1105I 1458 GACACAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2480 CGGCCG C^GGCCG
Eco52I 2480 CGGCCG C^GGCCG
Eco81I 1186 CCTCAGG CC^T[ACGT]AGG
FbaI 3250 TGATCA T^GATCA
Hin1I 2841 GACGCC G[GA]^CG[CT]C
MamI 2117 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4141 CAATTG C^AATTG
MroI 674 TCCGGA T^CCGGA
MunI 4141 CAATTG C^AATTG
NcoI 4165 CCATGG C^CATGG
NdeI 1768 CATATG CA^TATG
PflFI 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1005 ACCGGT A^CCGGT
PmaCI 1680 CACGTG CAC^GTG
PmlI 1680 CACGTG CAC^GTG
RcaI 3280 TCATGA T^CATGA
SexAI 3342 ACCAGGT A^CC[AT]GGT
SmaI 2731 CCCGGG CCC^GGG
SspBI 3810 TGTACA T^GTACA
SspI 4401 AATATT AAT^ATT
Tth111I 190 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1301 TCTAGA T^CTAGA
XmaCI 2731 CCCGGG C^CCGGG
XmaI 2731 CCCGGG C^CCGGG
XmnI 4399 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 489 GGTACC G^GTACC
2668 GGTACC
AcsI 1995 GAATTT [GA]^AATT[CT]
3284 GAATTT
ApoI 1995 GAATTT [GA]^AATT[CT]
3284 GAATTT
Asp718I 489 GGTACC G^GTACC
2668 GGTACC
BalI 3924 TGGCCA TGG^CCA
3938 TGGCCA
BamHI 1555 GGATCC G^GATCC
3588 GGATCC
BsaAI 1680 CACGTG [CT]AC^GT[GA]
3692 TACGTG
BsaWI 674 TCCGGA [AT]^CCGG[AT]
1005 ACCGGT
BsiEI 2480 CGGCCG CG[GA][CT]^CG
3219 CGGTCG
BspLU11I 1782 ACATGT A^CATGT
1846 ACATGT
BsrDI 2085 GCAATG GCAATG(2/0)
2419 GCAATG
BssSI 786 CACGAG CACGAG(-5/-1)
3182 CACGAG
BstEII 1465 GGTCACC G^GT[ACGT]ACC
2399 GGTCACC
BstPI 1465 GGTCACC G^GT[ACGT]ACC
2399 GGTCACC
EcoNI 1961 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3484 CCTGAAGCAGG
EcoO65I 1465 GGTCACC G^GT[ACGT]ACC
2399 GGTCACC
EcoT22I 3996 ATGCAT ATGCA^T
4376 ATGCAT
KpnI 489 GGTACC GGTAC^C
2668 GGTACC
MaeII 1681 ACGT A^CGT
3693 ACGT
MluNI 3924 TGGCCA TGG^CCA
3938 TGGCCA
MscI 3924 TGGCCA TGG^CCA
3938 TGGCCA
NaeI 78 GCCGGC GCC^GGC
2478 GCCGGC
NgoMIV 78 GCCGGC G^CCGGC
2478 GCCGGC
NsiI 3996 ATGCAT ATGCA^T
4376 ATGCAT
PaeR7I 1240 CTCGAG C^TCGAG
1397 CTCGAG
PflMI 2360 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3457 CCAGCACCTGG
PshAI 917 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3657 GACAACTGTC
SapI 312 GAAGAGC GCTCTTC(1/4)
2424 GAAGAGC
Van91I 2360 CCAATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3457 CCAGCACCTGG
XcmI 280 CCAAGGACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4036 CCATCCTGGTATTGG
XhoI 1240 CTCGAG C^TCGAG
1397 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII AviII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
SbfI ScrFI SfaNI SfcI SmlI
SphI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII