##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI AflII AhdI Alw44I
Aor51HI ApaLI AscI AspEI AvrII
BbrPI BcgI BfrI BlnI BsaAI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspMI BssHII BssSI
Bst1107I BstXI BstZ17I ClaI CpoI
Eam1105I Eco47III EcoRV EcoT22I FseI
HpaI MluI MroI NheI NotI
NruI NsiI PacI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI ScaI SexAI
SnaBI SpeI SphI SwaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3400 GACGTC GACGT^C
AccI 4011 GTAGAC GT^[AC][GT]AC
AflIII 407 ACATGT A^C[GA][CT]GT
AgeI 2408 ACCGGT A^CCGGT
AocI 3369 CCTCAGG CC^T[ACGT]AGG
AspI 4390 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 273 TGCGCA TGC^GCA
BbeI 62 GGCGCC GGCGC^C
BciVI 3130 GTATCC GTATCC(6/5)
BclI 2054 TGATCA T^GATCA
BlpI 3942 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3942 GCTCAGC GC^T[ACGT]AGC
BsaWI 2408 ACCGGT [AT]^CCGG[AT]
BsiEI 236 CGGCCG CG[GA][CT]^CG
BspLU11I 407 ACATGT A^CATGT
BsrBI 4025 GAGCGG CCGCTC(-3/-3)
BstBI 3870 TTCGAA TT^CGAA
Bsu36I 3369 CCTCAGG CC^T[ACGT]AGG
CelII 3942 GCTCAGC GC^T[ACGT]AGC
DraI 4557 TTTAAA TTT^AAA
DraIII 1696 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1292 GACATTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 236 CGGCCG C^GGCCG
EclXI 236 CGGCCG C^GGCCG
Eco52I 236 CGGCCG C^GGCCG
Eco81I 3369 CCTCAGG CC^T[ACGT]AGG
FbaI 2054 TGATCA T^GATCA
FspI 273 TGCGCA TGC^GCA
HincII 883 GTCAAC GT[CT]^[GA]AC
HindII 883 GTCAAC GT[CT]^[GA]AC
HindIII 14 AAGCTT A^AGCTT
KasI 62 GGCGCC G^GCGCC
MfeI 2983 CAATTG C^AATTG
MslI 1799 CATTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2983 CAATTG C^AATTG
NaeI 153 GCCGGC GCC^GGC
NarI 62 GGCGCC GG^CGCC
NcoI 813 CCATGG C^CATGG
NdeI 1828 CATATG CA^TATG
NgoMIV 153 GCCGGC G^CCGGC
NspV 3870 TTCGAA TT^CGAA
PaeR7I 1586 CTCGAG C^TCGAG
PflFI 4390 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2408 ACCGGT A^CCGGT
SbfI 947 CCTGCAGG CCTGCA^GG
SfiI 193 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 62 GGCGCC GGC^GCC
SfuI 3870 TTCGAA TT^CGAA
SgrAI 152 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 947 CCTGCAGG CCTGCA^GG
Tth111I 4390 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1586 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 461 GGTACC G^GTACC
2387 GGTACC
ApaI 201 GGGCCC GGGCC^C
259 GGGCCC
AseI 1800 ATTAAT AT^TAAT
3792 ATTAAT
AsnI 1800 ATTAAT AT^TAAT
3792 ATTAAT
Asp700I 296 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1486 GAACTCATTC
Asp718I 461 GGTACC G^GTACC
2387 GGTACC
BamHI 1887 GGATCC G^GATCC
2710 GGATCC
BbvCI 933 CCTCAGC CCTCAGC(-5/-2)
159 GCTGAGG
BglII 1438 AGATCT A^GATCT
3197 AGATCT
BsaBI 1498 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1971 GATGTGGATC
Bsp1407I 4120 TGTACA T^GTACA
4235 TGTACA
BsrDI 2670 GCAATG GCAATG(2/0)
4414 GCAATG
BsrFI 153 GCCGGC [GA]^CCGG[CT]
2408 ACCGGT
BsrGI 4120 TGTACA T^GTACA
4235 TGTACA
BstEII 1510 GGTCACC G^GT[ACGT]ACC
2162 GGTCACC
BstPI 1510 GGTCACC G^GT[ACGT]ACC
2162 GGTCACC
Cfr10I 153 GCCGGC [GA]^CCGG[CT]
2408 ACCGGT
EcoNI 1769 CCTTTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3739 CCTGGGAGAGG
EcoO65I 1510 GGTCACC G^GT[ACGT]ACC
2162 GGTCACC
EcoRI 3112 GAATTC G^AATTC
3820 GAATTC
KpnI 461 GGTACC GGTAC^C
2387 GGTACC
MamI 1498 GATCTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1971 GATGTGGATC
NspI 407 ACATGT [GA]CATG^[CT]
3938 ACATGC
PflMI 2346 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCACCAATTGG
PpuMI 1545 GGGTCCC [GA]G^G[AT]CC[CT]
3970 AGGACCC
PshBI 1800 ATTAAT AT^TAAT
3792 ATTAAT
SapI 1866 GCTCTTC GCTCTTC(1/4)
3159 GCTCTTC
SmaI 263 CCCGGG CCC^GGG
3081 CCCGGG
SspBI 4120 TGTACA T^GTACA
4235 TGTACA
SspI 2817 AATATT AAT^ATT
3297 AATATT
StuI 952 AGGCCT AGG^CCT
3429 AGGCCT
Van91I 2346 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2979 CCACCAATTGG
XcmI 2609 CCAGATTCCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3543 CCAGAGAAGCCTTGG
XmaCI 263 CCCGGG C^CCGGG
3081 CCCGGG
XmaI 263 CCCGGG C^CCGGG
3081 CCCGGG
XmnI 296 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1486 GAACTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII