##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AcyI AflII AflIII
AgeI AhdI AscI AseI AsnI
AspEI BalI BamHI BbeI BclI
BfrI BsaBI BsaHI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoRV FbaI FseI HgaI
Hin1I KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2407 GGTACC G^GTACC
AccI 2312 GTCTAC GT^[AC][GT]AC
AclI 4198 AACGTT AA^CGTT
Alw44I 2263 GTGCAC G^TGCAC
Aor51HI 2856 AGCGCT AGC^GCT
ApaLI 2263 GTGCAC G^TGCAC
Asp718I 2407 GGTACC G^GTACC
AspI 2325 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3420 CCTAGG C^CTAGG
BbrPI 2523 CACGTG CAC^GTG
BbvCI 3656 CCTCAGC CCTCAGC(-5/-2)
BcgI 2641 GCATTCCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2286 AGATCT A^GATCT
BlnI 3420 CCTAGG C^CTAGG
BlpI 4001 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4001 GCTGAGC GC^T[ACGT]AGC
BsaAI 2523 CACGTG [CT]AC^GT[GA]
BsaI 2323 GAGACC GGTCTC(1/5)
BsaWI 261 TCCGGT [AT]^CCGG[AT]
BsmBI 1078 GAGACG CGTCTC(1/5)
BspMI 1457 ACCTGC ACCTGC(4/8)
BssHII 2542 GCGCGC G^CGCGC
BstXI 1795 CCAATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4001 GCTGAGC GC^T[ACGT]AGC
Eco47III 2856 AGCGCT AGC^GCT
EcoT22I 4073 ATGCAT ATGCA^T
HincII 4188 GTTAAC GT[CT]^[GA]AC
HindII 4188 GTTAAC GT[CT]^[GA]AC
HindIII 2136 AAGCTT A^AGCTT
HpaI 4188 GTTAAC GTT^AAC
KpnI 2407 GGTACC GGTAC^C
NheI 2074 GCTAGC G^CTAGC
NsiI 4073 ATGCAT ATGCA^T
PflFI 2325 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2043 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2523 CACGTG CAC^GTG
PmlI 2523 CACGTG CAC^GTG
Psp1406I 4198 AACGTT AA^CGTT
SmaI 2171 CCCGGG CCC^GGG
Tth111I 2325 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2043 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 784 TCTAGA T^CTAGA
XmaCI 2171 CCCGGG C^CCGGG
XmaI 2171 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 1828 CGCG CG^CG
2543 CGCG
ApaI 2174 GGGCCC GGGCC^C
2827 GGGCCC
AviII 533 TGCGCA TGC^GCA
1856 TGCGCA
BciVI 3131 GGATAC GTATCC(6/5)
1932 GTATCC
BsmI 995 GAATGC GAATGC(1/-1)
2641 GCATTC
BsrBI 2181 CCGCTC CCGCTC(-3/-3)
899 GAGCGG
BssSI 1550 CTCGTG CACGAG(-5/-1)
2777 CTCGTG
BstEII 2674 GGTCACC G^GT[ACGT]ACC
3677 GGTGACC
BstPI 2674 GGTCACC G^GT[ACGT]ACC
3677 GGTGACC
BstUI 1828 CGCG CG^CG
2543 CGCG
EcoO65I 2674 GGTCACC G^GT[ACGT]ACC
3677 GGTGACC
EcoRI 2294 GAATTC G^AATTC
2834 GAATTC
FauI 454 GCGGG CCCGC(4/6)
1238 GCGGG
FspI 533 TGCGCA TGC^GCA
1856 TGCGCA
HaeII 2856 AGCGCT [GA]GCGC^[CT]
3998 GGCGCT
MvnI 1828 CGCG CG^CG
2543 CGCG
NcoI 36 CCATGG C^CATGG
3670 CCATGG
SapI 302 GCTCTTC GCTCTTC(1/4)
2110 GCTCTTC
SexAI 768 ACCTGGT A^CC[AT]GGT
2042 ACCAGGT
SmlI 202 CTCAAG C^T[CT][GA]AG
2333 CTCAAG
SspI 1387 AATATT AAT^ATT
3570 AATATT
XcmI 2246 CCATTGCAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4016 CCACCTTACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI