##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
ApaI AscI Asp700I Asp718I AspEI
AspI AviII BalI BamHI BbrPI
BglI BlpI Bpu1102I BsaBI BsiWI
BspDI BsrBI BssHII BstBI CelII
ClaI CpoI DraIII Eam1105I EcoRV
FseI FspI HpaI KpnI KspI
MamI MluI MluNI MscI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 3908 GTATAC GT^[AC][GT]AC
AccII 3561 CGCG CG^CG
Alw44I 3539 GTGCAC G^TGCAC
AocI 592 CCTTAGG CC^T[ACGT]AGG
Aor51HI 169 AGCGCT AGC^GCT
ApaLI 3539 GTGCAC G^TGCAC
AvaI 2180 CTCGGG C^[CT]CG[GA]G
AvrII 4291 CCTAGG C^CTAGG
BbeI 3608 GGCGCC GGCGC^C
BlnI 4291 CCTAGG C^CTAGG
BsaAI 4083 TACGTG [CT]AC^GT[GA]
BsiEI 1313 CGGCCG CG[GA][CT]^CG
BsoBI 2180 CTCGGG C^[CT]CG[GA]G
BspLU11I 3857 ACATGT A^CATGT
BsrDI 2360 GCAATG GCAATG(2/0)
BsrFI 1428 GCCGGT [GA]^CCGG[CT]
BssSI 1369 CACGAG CACGAG(-5/-1)
Bst1107I 3908 GTATAC GTA^TAC
BstEII 2411 GGTCACC G^GT[ACGT]ACC
BstPI 2411 GGTCACC G^GT[ACGT]ACC
BstUI 3561 CGCG CG^CG
BstZ17I 3908 GTATAC GTA^TAC
Bsu36I 592 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1428 GCCGGT [GA]^CCGG[CT]
DsaI 2378 CCATGG C^C[GA][CT]GG
EaeI 1313 CGGCCG [CT]^GGCC[GA]
EagI 1313 CGGCCG C^GGCCG
EclXI 1313 CGGCCG C^GGCCG
Eco47III 169 AGCGCT AGC^GCT
Eco52I 1313 CGGCCG C^GGCCG
Eco81I 592 CCTTAGG CC^T[ACGT]AGG
EcoNI 3643 CCTCCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2411 GGTCACC G^GT[ACGT]ACC
EcoRI 2508 GAATTC G^AATTC
EcoT22I 966 ATGCAT ATGCA^T
FauI 4659 CCCGC CCCGC(4/6)
KasI 3608 GGCGCC G^GCGCC
MfeI 1708 CAATTG C^AATTG
MunI 1708 CAATTG C^AATTG
MvnI 3561 CGCG CG^CG
NarI 3608 GGCGCC GG^CGCC
NcoI 2378 CCATGG C^CATGG
NdeI 1560 CATATG CA^TATG
NsiI 966 ATGCAT ATGCA^T
PflMI 1390 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1367 GACACGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1180 GAGCTC GAGCT^C
SexAI 4533 ACCTGGT A^CC[AT]GGT
SfoI 3608 GGCGCC GGC^GCC
StuI 1729 AGGCCT AGG^CCT
Van91I 1390 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1079 TCTAGA T^CTAGA
XcmI 3453 CCATGCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 2348 TCCGGA T^CCGGA
3419 TCCGGA
AflIII 2453 ACACGT A^C[GA][CT]GT
3857 ACATGT
AseI 3292 ATTAAT AT^TAAT
4383 ATTAAT
AsnI 3292 ATTAAT AT^TAAT
4383 ATTAAT
BbvCI 554 GCTGAGG CCTCAGC(-5/-2)
3199 GCTGAGG
BcgI 1371 CGAGTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
847 GCAGCGTTCTCG
BclI 265 TGATCA T^GATCA
3816 TGATCA
BglII 1825 AGATCT A^GATCT
3193 AGATCT
BseAI 2348 TCCGGA T^CCGGA
3419 TCCGGA
Bsp1407I 627 TGTACA T^GTACA
5179 TGTACA
BspEI 2348 TCCGGA T^CCGGA
3419 TCCGGA
BsrGI 627 TGTACA T^GTACA
5179 TGTACA
DrdI 4198 GACGATAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4493 GACATGGGAGTC
FbaI 265 TGATCA T^GATCA
3816 TGATCA
HgaI 2842 GACGC GACGC(5/10)
2540 GCGTC
MroI 2348 TCCGGA T^CCGGA
3419 TCCGGA
PshBI 3292 ATTAAT AT^TAAT
4383 ATTAAT
SspBI 627 TGTACA T^GTACA
5179 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BfrI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BspHI BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII