##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII BamHI BanI
BbeI BbrPI BbvCI BglI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BspEI BspLU11I BsrBI BsrDI
BssHII Bst1107I BstAPI BstBI BstUI
BstZ17I CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 1977 AACGTT AA^CGTT
AcyI 579 GACGCC G[GA]^CG[CT]C
AflII 1777 CTTAAG C^TTAAG
Aor51HI 1587 AGCGCT AGC^GCT
AvrII 1815 CCTAGG C^CTAGG
BalI 53 TGGCCA TGG^CCA
BcgI 587 CGACTGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2128 GGATAC GTATCC(6/5)
BfrI 1777 CTTAAG C^TTAAG
BglII 1422 AGATCT A^GATCT
BlnI 1815 CCTAGG C^CTAGG
BsaHI 579 GACGCC G[GA]^CG[CT]C
BseRI 1618 GAGGAG GAGGAG(10/8)
Bsp1407I 2386 TGTACA T^GTACA
BspDI 1219 ATCGAT AT^CGAT
BspHI 1163 TCATGA T^CATGA
BsrFI 3879 GCCGGT [GA]^CCGG[CT]
BsrGI 2386 TGTACA T^GTACA
BssSI 2999 CACGAG CACGAG(-5/-1)
Cfr10I 3879 GCCGGT [GA]^CCGG[CT]
ClaI 1219 ATCGAT AT^CGAT
EaeI 53 TGGCCA [CT]^GGCC[GA]
Eco47III 1587 AGCGCT AGC^GCT
EcoRI 3361 GAATTC G^AATTC
FauI 2177 CCCGC CCCGC(4/6)
Hin1I 579 GACGCC G[GA]^CG[CT]C
HincII 2089 GTTGAC GT[CT]^[GA]AC
HindII 2089 GTTGAC GT[CT]^[GA]AC
MluNI 53 TGGCCA TGG^CCA
MscI 53 TGGCCA TGG^CCA
MslI 1384 CATCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 632 CCATGG C^CATGG
Psp1406I 1977 AACGTT AA^CGTT
RcaI 1163 TCATGA T^CATGA
ScaI 3982 AGTACT AGT^ACT
SphI 3500 GCATGC GCATG^C
SspBI 2386 TGTACA T^GTACA
XbaI 963 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AlwNI 989 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1753 CAGCAGCTG
AocI 2798 CCTGAGG CC^T[ACGT]AGG
3909 CCTGAGG
Asp700I 2239 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2322 GAAATAGTTC
BsgI 134 CTGCAC GTGCAG(16/14)
1943 GTGCAG
BsmI 1389 GAATGC GAATGC(1/-1)
3403 GCATTC
BspMI 3379 ACCTGC ACCTGC(4/8)
2547 GCAGGT
BstXI 1865 CCAATCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3323 CCAACTTCATGG
Bsu36I 2798 CCTGAGG CC^T[ACGT]AGG
3909 CCTGAGG
CfoI 1588 GCGC GCG^C
3394 GCGC
DsaI 632 CCATGG C^C[GA][CT]GG
3341 CCGTGG
Eco81I 2798 CCTGAGG CC^T[ACGT]AGG
3909 CCTGAGG
EcoNI 3725 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3741 CCTGGGTAAGG
EcoT14I 632 CCATGG C^C[AT][AT]GG
1815 CCTAGG
HaeII 1587 AGCGCT [GA]GCGC^[CT]
3393 AGCGCC
HgaI 579 GACGC GACGC(5/10)
3892 GCGTC
HhaI 1588 GCGC GCG^C
3394 GCGC
HinP1I 1588 GCGC G^CGC
3394 GCGC
NspI 3500 GCATGC [GA]CATG^[CT]
3760 GCATGT
SacI 5 GAGCTC GAGCT^C
1786 GAGCTC
SapI 1055 GAAGAGC GCTCTTC(1/4)
3229 GCTCTTC
SexAI 2398 ACCTGGT A^CC[AT]GGT
3676 ACCTGGT
SmaI 2407 CCCGGG CCC^GGG
3814 CCCGGG
SspI 2241 AATATT AAT^ATT
3533 AATATT
StyI 632 CCATGG C^C[AT][AT]GG
1815 CCTAGG
XcmI 749 CCAATTATCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1883 CCATTAAACAGCTGG
XmaCI 2407 CCCGGG C^CCGGG
3814 CCCGGG
XmaI 2407 CCCGGG C^CCGGG
3814 CCCGGG
XmnI 2239 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2322 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbsI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstEII BstF5I BstNI BstPI
BstYI Cac8I CelII Cfr13I DdeI
DpnII DraI DraII DraIII EarI
EcoO109I EcoO65I EcoRII FbaI Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII