##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AscI AseI AsnI Asp718I
AviII BamHI BbeI BsaBI BsaHI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII BstUI ClaI
CpoI DraI EcoNI EcoRV EcoT22I
FspI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NotI
NruI NsiI PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 558 GAATTC [GA]^AATT[CT]
AflII 1853 CTTAAG C^TTAAG
AgeI 3460 ACCGGT A^CCGGT
Alw44I 3641 GTGCAC G^TGCAC
ApaLI 3641 GTGCAC G^TGCAC
ApoI 558 GAATTC [GA]^AATT[CT]
AspI 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2246 CCTAGG C^CTAGG
BbrPI 81 CACGTG CAC^GTG
BbvCI 693 GCTGAGG CCTCAGC(-5/-2)
BcgI 2804 GCAGGCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1853 CTTAAG C^TTAAG
BglI 2705 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2246 CCTAGG C^CTAGG
BsmBI 3710 GAGACG CGTCTC(1/5)
BsrBI 3571 CCGCTC CCGCTC(-3/-3)
Bst1107I 2744 GTATAC GTA^TAC
BstBI 2106 TTCGAA TT^CGAA
BstEII 1338 GGTGACC G^GT[ACGT]ACC
BstPI 1338 GGTGACC G^GT[ACGT]ACC
BstZ17I 2744 GTATAC GTA^TAC
DrdI 4206 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1338 GGTGACC G^GT[ACGT]ACC
EcoRI 558 GAATTC G^AATTC
FseI 1474 GGCCGGCC GGCCGG^CC
NdeI 3122 CATATG CA^TATG
NspV 2106 TTCGAA TT^CGAA
PflFI 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3460 ACCGGT A^CCGGT
PmaCI 81 CACGTG CAC^GTG
PmlI 81 CACGTG CAC^GTG
SfiI 2704 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 2106 TTCGAA TT^CGAA
SspI 606 AATATT AAT^ATT
Tth111I 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1200 CCAAGTCGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 282 GTCTAC GT^[AC][GT]AC
2744 GTATAC
AflIII 82 ACGTGT A^C[GA][CT]GT
1323 ACACGT
AhdI 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3448 GACACACAGTC
Aor51HI 3783 AGCGCT AGC^GCT
3875 AGCGCT
Asp700I 208 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1083 GAAGAACTTC
AspEI 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3448 GACACACAGTC
BciVI 667 GTATCC GTATCC(6/5)
2304 GTATCC
BclI 1360 TGATCA T^GATCA
2128 TGATCA
BglII 1494 AGATCT A^GATCT
2401 AGATCT
BlpI 1569 GCTCAGC GC^T[ACGT]AGC
3676 GCTGAGC
Bpu1102I 1569 GCTCAGC GC^T[ACGT]AGC
3676 GCTGAGC
BsaWI 2968 TCCGGT [AT]^CCGG[AT]
3460 ACCGGT
BsiEI 1473 CGGCCG CG[GA][CT]^CG
1695 CGGCCG
BspHI 1357 TCATGA T^CATGA
2332 TCATGA
BsrDI 2383 CATTGC GCAATG(2/0)
721 GCAATG
BssSI 2568 CACGAG CACGAG(-5/-1)
2823 CTCGTG
CelII 1569 GCTCAGC GC^T[ACGT]AGC
3676 GCTGAGC
EagI 1473 CGGCCG C^GGCCG
1695 CGGCCG
Eam1105I 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3448 GACACACAGTC
EclXI 1473 CGGCCG C^GGCCG
1695 CGGCCG
Eco47III 3783 AGCGCT AGC^GCT
3875 AGCGCT
Eco52I 1473 CGGCCG C^GGCCG
1695 CGGCCG
FbaI 1360 TGATCA T^GATCA
2128 TGATCA
NaeI 1471 GCCGGC GCC^GGC
1475 GCCGGC
NgoMIV 1471 GCCGGC G^CCGGC
1475 GCCGGC
NheI 150 GCTAGC G^CTAGC
764 GCTAGC
RcaI 1357 TCATGA T^CATGA
2332 TCATGA
SapI 1930 GCTCTTC GCTCTTC(1/4)
1951 GCTCTTC
SmaI 1527 CCCGGG CCC^GGG
1999 CCCGGG
SphI 704 GCATGC GCATG^C
3490 GCATGC
XmaCI 1527 CCCGGG C^CCGGG
1999 CCCGGG
XmaI 1527 CCCGGG C^CCGGG
1999 CCCGGG
XmnI 208 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1083 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII