################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AscI AseI AsnI Asp718I AviII BamHI BbeI BsaBI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BstUI ClaI CpoI DraI EcoNI EcoRV EcoT22I FspI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NotI NruI NsiI PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcsI 558 GAATTC [GA]^AATT[CT] AflII 1853 CTTAAG C^TTAAG AgeI 3460 ACCGGT A^CCGGT Alw44I 3641 GTGCAC G^TGCAC ApaLI 3641 GTGCAC G^TGCAC ApoI 558 GAATTC [GA]^AATT[CT] AspI 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2246 CCTAGG C^CTAGG BbrPI 81 CACGTG CAC^GTG BbvCI 693 GCTGAGG CCTCAGC(-5/-2) BcgI 2804 GCAGGCGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1853 CTTAAG C^TTAAG BglI 2705 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2246 CCTAGG C^CTAGG BsmBI 3710 GAGACG CGTCTC(1/5) BsrBI 3571 CCGCTC CCGCTC(-3/-3) Bst1107I 2744 GTATAC GTA^TAC BstBI 2106 TTCGAA TT^CGAA BstEII 1338 GGTGACC G^GT[ACGT]ACC BstPI 1338 GGTGACC G^GT[ACGT]ACC BstZ17I 2744 GTATAC GTA^TAC DrdI 4206 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1338 GGTGACC G^GT[ACGT]ACC EcoRI 558 GAATTC G^AATTC FseI 1474 GGCCGGCC GGCCGG^CC NdeI 3122 CATATG CA^TATG NspV 2106 TTCGAA TT^CGAA PflFI 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3460 ACCGGT A^CCGGT PmaCI 81 CACGTG CAC^GTG PmlI 81 CACGTG CAC^GTG SfiI 2704 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 2106 TTCGAA TT^CGAA SspI 606 AATATT AAT^ATT Tth111I 193 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1200 CCAAGTCGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 282 GTCTAC GT^[AC][GT]AC 2744 GTATAC AflIII 82 ACGTGT A^C[GA][CT]GT 1323 ACACGT AhdI 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3448 GACACACAGTC Aor51HI 3783 AGCGCT AGC^GCT 3875 AGCGCT Asp700I 208 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1083 GAAGAACTTC AspEI 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3448 GACACACAGTC BciVI 667 GTATCC GTATCC(6/5) 2304 GTATCC BclI 1360 TGATCA T^GATCA 2128 TGATCA BglII 1494 AGATCT A^GATCT 2401 AGATCT BlpI 1569 GCTCAGC GC^T[ACGT]AGC 3676 GCTGAGC Bpu1102I 1569 GCTCAGC GC^T[ACGT]AGC 3676 GCTGAGC BsaWI 2968 TCCGGT [AT]^CCGG[AT] 3460 ACCGGT BsiEI 1473 CGGCCG CG[GA][CT]^CG 1695 CGGCCG BspHI 1357 TCATGA T^CATGA 2332 TCATGA BsrDI 2383 CATTGC GCAATG(2/0) 721 GCAATG BssSI 2568 CACGAG CACGAG(-5/-1) 2823 CTCGTG CelII 1569 GCTCAGC GC^T[ACGT]AGC 3676 GCTGAGC EagI 1473 CGGCCG C^GGCCG 1695 CGGCCG Eam1105I 2552 GACAGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3448 GACACACAGTC EclXI 1473 CGGCCG C^GGCCG 1695 CGGCCG Eco47III 3783 AGCGCT AGC^GCT 3875 AGCGCT Eco52I 1473 CGGCCG C^GGCCG 1695 CGGCCG FbaI 1360 TGATCA T^GATCA 2128 TGATCA NaeI 1471 GCCGGC GCC^GGC 1475 GCCGGC NgoMIV 1471 GCCGGC G^CCGGC 1475 GCCGGC NheI 150 GCTAGC G^CTAGC 764 GCTAGC RcaI 1357 TCATGA T^CATGA 2332 TCATGA SapI 1930 GCTCTTC GCTCTTC(1/4) 1951 GCTCTTC SmaI 1527 CCCGGG CCC^GGG 1999 CCCGGG SphI 704 GCATGC GCATG^C 3490 GCATGC XmaCI 1527 CCCGGG C^CCGGG 1999 CCCGGG XmaI 1527 CCCGGG C^CCGGG 1999 CCCGGG XmnI 208 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1083 GAAGAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII