################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccII AclI AflII AflIII AgeI AlwNI AocI ApaI AscI AseI AsnI Asp718I AvaI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BsgI BsiEI BsiWI BsoBI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I CpoI DraIII DrdI EaeI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FseI HincII HindII HpaI KpnI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NdeI NgoMIV NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 2338 GTAGAC GT^[AC][GT]AC AccIII 185 TCCGGA T^CCGGA AcyI 1165 GGCGCC G[GA]^CG[CT]C AhdI 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2662 GTGCAC G^TGCAC Aor51HI 1905 AGCGCT AGC^GCT ApaLI 2662 GTGCAC G^TGCAC AspEI 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 202 TGCGCA TGC^GCA BbeI 1165 GGCGCC GGCGC^C BclI 1053 TGATCA T^GATCA BcnI 2091 CCCGG CC^[GC]GG BglII 2081 AGATCT A^GATCT BlpI 1574 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1574 GCTAAGC GC^T[ACGT]AGC BsaAI 366 TACGTG [CT]AC^GT[GA] BsaHI 1165 GGCGCC G[GA]^CG[CT]C BsaWI 185 TCCGGA [AT]^CCGG[AT] BseAI 185 TCCGGA T^CCGGA Bsp1407I 3070 TGTACA T^GTACA BspEI 185 TCCGGA T^CCGGA BspMI 1004 ACCTGC ACCTGC(4/8) BsrDI 2641 CATTGC GCAATG(2/0) BsrGI 3070 TGTACA T^GTACA BstBI 531 TTCGAA TT^CGAA CelII 1574 GCTAAGC GC^T[ACGT]AGC DsaI 726 CCATGG C^C[GA][CT]GG Eam1105I 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1905 AGCGCT AGC^GCT EcoRI 2996 GAATTC G^AATTC EcoT22I 2306 ATGCAT ATGCA^T FbaI 1053 TGATCA T^GATCA FspI 202 TGCGCA TGC^GCA HgaI 1979 GACGC GACGC(5/10) Hin1I 1165 GGCGCC G[GA]^CG[CT]C KasI 1165 GGCGCC G^GCGCC MroI 185 TCCGGA T^CCGGA MslI 1323 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1165 GGCGCC GG^CGCC NciI 2091 CCCGG CC^[GC]GG NcoI 726 CCATGG C^CATGG NheI 1982 GCTAGC G^CTAGC NsiI 2306 ATGCAT ATGCA^T NspI 2308 GCATGT [GA]CATG^[CT] NspV 531 TTCGAA TT^CGAA SapI 1484 GAAGAGC GCTCTTC(1/4) SfoI 1165 GGCGCC GGC^GCC SfuI 531 TTCGAA TT^CGAA SspBI 3070 TGTACA T^GTACA SspI 1067 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Asp700I 524 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1066 GAATATTTTC AspI 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1525 GACTGAGTC BanI 1165 GGCGCC G^G[CT][GA]CC 2636 GGCACC BanII 48 GAGCTC G[GA]GC[CT]^C 1471 GAGCTC BpmI 2676 CTCCAG CTGGAG(16/14) 1172 CTGGAG BsmBI 1828 GAGACG CGTCTC(1/5) 1977 GAGACG BsmI 2304 GAATGC GAATGC(1/-1) 2264 GCATTC BspDI 1196 ATCGAT AT^CGAT 1211 ATCGAT BstXI 506 CCAACGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1322 CCATCAAAATGG ClaI 1196 ATCGAT AT^CGAT 1211 ATCGAT EcoT14I 701 CCAAGG C^C[AT][AT]GG 726 CCATGG FauI 664 GCGGG CCCGC(4/6) 1702 GCGGG HphI 2597 GGTGA GGTGA(8/7) 1892 TCACC MspA1I 662 CAGCGG C[AC]G^C[GT]G 1700 CAGCGG PflFI 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1525 GACTGAGTC PstI 491 CTGCAG CTGCA^G 1697 CTGCAG SacI 48 GAGCTC GAGCT^C 1471 GAGCTC ScaI 1545 AGTACT AGT^ACT 2740 AGTACT SmlI 650 CTCAAG C^T[CT][GA]AG 2391 CTCAAG StyI 701 CCAAGG C^C[AT][AT]GG 726 CCATGG Tth111I 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1525 GACTGAGTC XmnI 524 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1066 GAATATTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaBI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII