##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AlwNI AocI ApaI
AscI AseI AsnI Asp718I AvaI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BfrI BglI BlnI
BsgI BsiEI BsiWI BsoBI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I CpoI DraIII DrdI
EaeI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRV FseI HincII
HindII HpaI KpnI KspI MfeI
MluI MluNI MscI MunI MvnI
NaeI NdeI NgoMIV NotI NruI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 2338 GTAGAC GT^[AC][GT]AC
AccIII 185 TCCGGA T^CCGGA
AcyI 1165 GGCGCC G[GA]^CG[CT]C
AhdI 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2662 GTGCAC G^TGCAC
Aor51HI 1905 AGCGCT AGC^GCT
ApaLI 2662 GTGCAC G^TGCAC
AspEI 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 202 TGCGCA TGC^GCA
BbeI 1165 GGCGCC GGCGC^C
BclI 1053 TGATCA T^GATCA
BcnI 2091 CCCGG CC^[GC]GG
BglII 2081 AGATCT A^GATCT
BlpI 1574 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1574 GCTAAGC GC^T[ACGT]AGC
BsaAI 366 TACGTG [CT]AC^GT[GA]
BsaHI 1165 GGCGCC G[GA]^CG[CT]C
BsaWI 185 TCCGGA [AT]^CCGG[AT]
BseAI 185 TCCGGA T^CCGGA
Bsp1407I 3070 TGTACA T^GTACA
BspEI 185 TCCGGA T^CCGGA
BspMI 1004 ACCTGC ACCTGC(4/8)
BsrDI 2641 CATTGC GCAATG(2/0)
BsrGI 3070 TGTACA T^GTACA
BstBI 531 TTCGAA TT^CGAA
CelII 1574 GCTAAGC GC^T[ACGT]AGC
DsaI 726 CCATGG C^C[GA][CT]GG
Eam1105I 2600 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1905 AGCGCT AGC^GCT
EcoRI 2996 GAATTC G^AATTC
EcoT22I 2306 ATGCAT ATGCA^T
FbaI 1053 TGATCA T^GATCA
FspI 202 TGCGCA TGC^GCA
HgaI 1979 GACGC GACGC(5/10)
Hin1I 1165 GGCGCC G[GA]^CG[CT]C
KasI 1165 GGCGCC G^GCGCC
MroI 185 TCCGGA T^CCGGA
MslI 1323 CATCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1165 GGCGCC GG^CGCC
NciI 2091 CCCGG CC^[GC]GG
NcoI 726 CCATGG C^CATGG
NheI 1982 GCTAGC G^CTAGC
NsiI 2306 ATGCAT ATGCA^T
NspI 2308 GCATGT [GA]CATG^[CT]
NspV 531 TTCGAA TT^CGAA
SapI 1484 GAAGAGC GCTCTTC(1/4)
SfoI 1165 GGCGCC GGC^GCC
SfuI 531 TTCGAA TT^CGAA
SspBI 3070 TGTACA T^GTACA
SspI 1067 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 524 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1066 GAATATTTTC
AspI 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACTGAGTC
BanI 1165 GGCGCC G^G[CT][GA]CC
2636 GGCACC
BanII 48 GAGCTC G[GA]GC[CT]^C
1471 GAGCTC
BpmI 2676 CTCCAG CTGGAG(16/14)
1172 CTGGAG
BsmBI 1828 GAGACG CGTCTC(1/5)
1977 GAGACG
BsmI 2304 GAATGC GAATGC(1/-1)
2264 GCATTC
BspDI 1196 ATCGAT AT^CGAT
1211 ATCGAT
BstXI 506 CCAACGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1322 CCATCAAAATGG
ClaI 1196 ATCGAT AT^CGAT
1211 ATCGAT
EcoT14I 701 CCAAGG C^C[AT][AT]GG
726 CCATGG
FauI 664 GCGGG CCCGC(4/6)
1702 GCGGG
HphI 2597 GGTGA GGTGA(8/7)
1892 TCACC
MspA1I 662 CAGCGG C[AC]G^C[GT]G
1700 CAGCGG
PflFI 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACTGAGTC
PstI 491 CTGCAG CTGCA^G
1697 CTGCAG
SacI 48 GAGCTC GAGCT^C
1471 GAGCTC
ScaI 1545 AGTACT AGT^ACT
2740 AGTACT
SmlI 650 CTCAAG C^T[CT][GA]AG
2391 CTCAAG
StyI 701 CCAAGG C^C[AT][AT]GG
726 CCATGG
Tth111I 280 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1525 GACTGAGTC
XmnI 524 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1066 GAATATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaBI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII