##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AgeI AocI Aor51HI ApaI
AscI AseI AsnI AspI AviII
BbeI BbrPI BsaAI BsiEI BsiWI
BsmI BspDI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I FseI FspI HpaI KasI
KspI MfeI MluI MunI NarI
NotI NruI NsiI PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RsrII SacII SbfI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4407 GGTACC G^GTACC
AccII 3514 CGCG CG^CG
AccIII 3822 TCCGGA T^CCGGA
AclI 4013 AACGTT AA^CGTT
AcyI 2744 GACGCC G[GA]^CG[CT]C
AflII 4699 CTTAAG C^TTAAG
Asp718I 4407 GGTACC G^GTACC
AvrII 942 CCTAGG C^CTAGG
BamHI 1792 GGATCC G^GATCC
BbvCI 3668 GCTGAGG CCTCAGC(-5/-2)
BciVI 2853 GTATCC GTATCC(6/5)
BfrI 4699 CTTAAG C^TTAAG
BglI 4160 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 942 CCTAGG C^CTAGG
BsaHI 2744 GACGCC G[GA]^CG[CT]C
BsaWI 3822 TCCGGA [AT]^CCGG[AT]
BseAI 3822 TCCGGA T^CCGGA
BsmBI 2072 CGTCTC CGTCTC(1/5)
Bsp1407I 2574 TGTACA T^GTACA
BspEI 3822 TCCGGA T^CCGGA
BspLU11I 3369 ACATGT A^CATGT
BsrFI 2409 GCCGGC [GA]^CCGG[CT]
BsrGI 2574 TGTACA T^GTACA
BstBI 3597 TTCGAA TT^CGAA
BstUI 3514 CGCG CG^CG
BstXI 2262 CCAAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2409 GCCGGC [GA]^CCGG[CT]
HaeII 3552 AGCGCC [GA]GCGC^[CT]
Hin1I 2744 GACGCC G[GA]^CG[CT]C
HincII 3935 GTCGAC GT[CT]^[GA]AC
HindII 3935 GTCGAC GT[CT]^[GA]AC
KpnI 4407 GGTACC GGTAC^C
MroI 3822 TCCGGA T^CCGGA
MvnI 3514 CGCG CG^CG
NaeI 2409 GCCGGC GCC^GGC
NcoI 2966 CCATGG C^CATGG
NdeI 1732 CATATG CA^TATG
NgoMIV 2409 GCCGGC G^CCGGC
NheI 3647 GCTAGC G^CTAGC
NspI 3369 ACATGT [GA]CATG^[CT]
NspV 3597 TTCGAA TT^CGAA
PaeR7I 2177 CTCGAG C^TCGAG
Psp1406I 4013 AACGTT AA^CGTT
SalI 3935 GTCGAC G^TCGAC
SapI 201 GAAGAGC GCTCTTC(1/4)
SexAI 1041 ACCTGGT A^CC[AT]GGT
SfuI 3597 TTCGAA TT^CGAA
SspBI 2574 TGTACA T^GTACA
SwaI 1662 ATTTAAAT ATTT^AAAT
XhoI 2177 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 438 GTAGAC GT^[AC][GT]AC
3935 GTCGAC
AflIII 1980 ACACGT A^C[GA][CT]GT
3369 ACATGT
AlwNI 3203 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
3863 CAGGTCCTG
BcgI 3188 CGACAGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4574 CGATCCTCCTGC
BclI 1564 TGATCA T^GATCA
2847 TGATCA
BglII 3192 AGATCT A^GATCT
3816 AGATCT
BsaBI 2848 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3998 GATGCCAATC
BspHI 1999 TCATGA T^CATGA
3883 TCATGA
BstAPI 1079 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3417 GCACTGGCTGC
DraI 1277 TTTAAA TTT^AAA
1663 TTTAAA
DsaI 2966 CCATGG C^C[GA][CT]GG
4230 CCACGG
EarI 3767 CTCTTC CTCTTC(1/4)
201 GAAGAG
EcoNI 2446 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3889 CCTTCTGCAGG
EcoRV 2024 GATATC GAT^ATC
3149 GATATC
FauI 244 GCGGG CCCGC(4/6)
1894 GCGGG
FbaI 1564 TGATCA T^GATCA
2847 TGATCA
HgaI 2744 GACGC GACGC(5/10)
2905 GCGTC
HindIII 1112 AAGCTT A^AGCTT
1352 AAGCTT
Ksp632I 3767 CTCTTC CTCTTC(1/4)
201 GAAGAG
MamI 2848 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3998 GATGCCAATC
PvuII 1245 CAGCTG CAG^CTG
2589 CAGCTG
RcaI 1999 TCATGA T^CATGA
3883 TCATGA
SmaI 256 CCCGGG CCC^GGG
3045 CCCGGG
SspI 364 AATATT AAT^ATT
3161 AATATT
StuI 2655 AGGCCT AGG^CCT
3310 AGGCCT
XcmI 3378 CCAGAGAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3915 CCATTTACAACATGG
XmaCI 256 CCCGGG C^CCGGG
3045 CCCGGG
XmaI 256 CCCGGG C^CCGGG
3045 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspEI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII EaeI Eam1105I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI