##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AlwNI ApaI AscI Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BcgI BclI BfrI BlnI
BsiWI BspDI BssHII ClaI CpoI
Eam1105I EcoNI EcoRI EcoRV FbaI
FseI FspI HpaI KasI KpnI
MluI NarI NcoI NruI PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SalI SbfI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcyI 207 GGCGTC G[GA]^CG[CT]C
Alw44I 1052 GTGCAC G^TGCAC
AlwI 3819 GATCC GGATC(4/5)
Aor51HI 3727 AGCGCT AGC^GCT
ApaLI 1052 GTGCAC G^TGCAC
AseI 3404 ATTAAT AT^TAAT
AsnI 3404 ATTAAT AT^TAAT
AspI 3222 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 423 GAGCCC G[GA]GC[CT]^C
BbrPI 1116 CACGTG CAC^GTG
BglI 158 GCCATCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4642 AGATCT A^GATCT
BsaBI 4556 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 207 GGCGTC G[GA]^CG[CT]C
BsmBI 58 GAGACG CGTCTC(1/5)
BspLU11I 3525 ACATGT A^CATGT
BsrBI 366 CCGCTC CCGCTC(-3/-3)
BsrFI 506 GCCGGC [GA]^CCGG[CT]
BssSI 3048 CACGAG CACGAG(-5/-1)
Bst1107I 2552 GTATAC GTA^TAC
BstBI 1935 TTCGAA TT^CGAA
BstEII 2237 GGTGACC G^GT[ACGT]ACC
BstPI 2237 GGTGACC G^GT[ACGT]ACC
BstYI 4642 AGATCT [GA]^GATC[CT]
BstZ17I 2552 GTATAC GTA^TAC
Cfr10I 506 GCCGGC [GA]^CCGG[CT]
DraI 657 TTTAAA TTT^AAA
DraIII 4803 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3727 AGCGCT AGC^GCT
EcoO65I 2237 GGTGACC G^GT[ACGT]ACC
Hin1I 207 GGCGTC G[GA]^CG[CT]C
MamI 4556 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 4642 AGATCT [GA]^GATC[CT]
NaeI 506 GCCGGC GCC^GGC
NgoMIV 506 GCCGGC G^CCGGC
NheI 3114 GCTAGC G^CTAGC
NotI 404 GCGGCCGC GC^GGCCGC
NspV 1935 TTCGAA TT^CGAA
PflFI 3222 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2914 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1116 CACGTG CAC^GTG
PmlI 1116 CACGTG CAC^GTG
PshBI 3404 ATTAAT AT^TAAT
PvuII 3595 CAGCTG CAG^CTG
SapI 2781 GAAGAGC GCTCTTC(1/4)
ScaI 2860 AGTACT AGT^ACT
SexAI 3546 ACCTGGT A^CC[AT]GGT
SfuI 1935 TTCGAA TT^CGAA
SmaI 443 CCCGGG CCC^GGG
SspI 923 AATATT AAT^ATT
StuI 2672 AGGCCT AGG^CCT
Tth111I 3222 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2914 CCAGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3741 CCACAGATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 4642 AGATCT [GA]^GATC[CT]
XmaCI 443 CCCGGG C^CCGGG
XmaI 443 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 823 TCCGGA T^CCGGA
3290 TCCGGA
AflIII 1115 ACACGT A^C[GA][CT]GT
3525 ACATGT
AocI 2252 CCTGAGG CC^T[ACGT]AGG
3868 CCTGAGG
Asp700I 1104 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4151 GAAGTAGTTC
BalI 1574 TGGCCA TGG^CCA
2627 TGGCCA
BciVI 2533 GTATCC GTATCC(6/5)
4605 GTATCC
BsaAI 1116 CACGTG [CT]AC^GT[GA]
2693 CACGTA
BseAI 823 TCCGGA T^CCGGA
3290 TCCGGA
BsmI 3719 GAATGC GAATGC(1/-1)
4136 GAATGC
BspEI 823 TCCGGA T^CCGGA
3290 TCCGGA
BspHI 2816 TCATGA T^CATGA
4132 TCATGA
BstAPI 1045 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1213 GCAAGCGATGC
Bsu36I 2252 CCTGAGG CC^T[ACGT]AGG
3868 CCTGAGG
DrdI 3099 GACTCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3982 GACTGGTCAGTC
EagI 261 CGGCCG C^GGCCG
405 CGGCCG
EclXI 261 CGGCCG C^GGCCG
405 CGGCCG
Eco52I 261 CGGCCG C^GGCCG
405 CGGCCG
Eco81I 2252 CCTGAGG CC^T[ACGT]AGG
3868 CCTGAGG
MaeII 1117 ACGT A^CGT
2694 ACGT
MfeI 3766 CAATTG C^AATTG
4620 CAATTG
MluNI 1574 TGGCCA TGG^CCA
2627 TGGCCA
MroI 823 TCCGGA T^CCGGA
3290 TCCGGA
MscI 1574 TGGCCA TGG^CCA
2627 TGGCCA
MunI 3766 CAATTG C^AATTG
4620 CAATTG
PaeR7I 414 CTCGAG C^TCGAG
523 CTCGAG
RcaI 2816 TCATGA T^CATGA
4132 TCATGA
XbaI 496 TCTAGA T^CTAGA
1794 TCTAGA
XhoI 414 CTCGAG C^TCGAG
523 CTCGAG
XmnI 1104 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4151 GAAGTAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI ApoI AspHI AvaI AvaII
BbsI BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I