##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AscI AseI AsnI Asp700I
AviII AvrII BamHI BbvCI BclI
BfrI BlnI BsiEI BsiWI BsmBI
BspDI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DrdI EagI EclXI Eco52I
EcoRV EcoT22I FbaI FseI FspI
HpaI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SalI ScaI SexAI SfiI SfuI
SgrAI SpeI SphI SspI SwaI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 700 TCCGGA T^CCGGA
AhdI 1646 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 874 GTGCAC G^TGCAC
AocI 1845 CCTCAGG CC^T[ACGT]AGG
ApaLI 874 GTGCAC G^TGCAC
AspEI 1646 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2600 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1815 CACGTG CAC^GTG
BcgI 2139 CGAGACCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2922 AGATCT A^GATCT
BsaBI 3745 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 700 TCCGGA T^CCGGA
Bsp1407I 619 TGTACA T^GTACA
BspEI 700 TCCGGA T^CCGGA
BspHI 1373 TCATGA T^CATGA
BsrDI 4072 CATTGC GCAATG(2/0)
BsrGI 619 TGTACA T^GTACA
BssSI 1934 CACGAG CACGAG(-5/-1)
BstEII 4125 GGTGACC G^GT[ACGT]ACC
BstPI 4125 GGTGACC G^GT[ACGT]ACC
Bsu36I 1845 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1646 GACCTTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1845 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4125 GGTGACC G^GT[ACGT]ACC
EcoRI 4201 GAATTC G^AATTC
HindIII 2481 AAGCTT A^AGCTT
KspI 2700 CCGCGG CCGC^GG
MamI 3745 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 700 TCCGGA T^CCGGA
NspI 1396 ACATGC [GA]CATG^[CT]
PaeR7I 2137 CTCGAG C^TCGAG
PflFI 2600 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1815 CACGTG CAC^GTG
PmlI 1815 CACGTG CAC^GTG
PshAI 3156 GACTAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1373 TCATGA T^CATGA
SacII 2700 CCGCGG CCGC^GG
SbfI 3055 CCTGCAGG CCTGCA^GG
SnaBI 2187 TACGTA TAC^GTA
Sse8387I 3055 CCTGCAGG CCTGCA^GG
SspBI 619 TGTACA T^GTACA
StuI 1789 AGGCCT AGG^CCT
Tth111I 2600 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2137 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 302 GGTACC G^GTACC
668 GGTACC
AcyI 797 GGCGCC G[GA]^CG[CT]C
2834 GGCGCC
Aor51HI 927 AGCGCT AGC^GCT
1011 AGCGCT
Asp718I 302 GGTACC G^GTACC
668 GGTACC
BbeI 797 GGCGCC GGCGC^C
2834 GGCGCC
BbsI 1389 GTCTTC GAAGAC(2/6)
1781 GTCTTC
BciVI 2385 GGATAC GTATCC(6/5)
2263 GTATCC
BglI 210 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2014 GCCCACACGGC
BlpI 19 GCTGAGC GC^T[ACGT]AGC
2023 GCTCAGC
Bpu1102I 19 GCTGAGC GC^T[ACGT]AGC
2023 GCTCAGC
BpuAI 1389 GTCTTC GAAGAC(2/6)
1781 GTCTTC
BsaAI 1815 CACGTG [CT]AC^GT[GA]
2187 TACGTA
BsaHI 797 GGCGCC G[GA]^CG[CT]C
2834 GGCGCC
BstXI 1715 CCACCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3209 CCAGGGTGTTGG
CelII 19 GCTGAGC GC^T[ACGT]AGC
2023 GCTCAGC
DraIII 386 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1301 CACAGTGTG
Eco47III 927 AGCGCT AGC^GCT
1011 AGCGCT
Hin1I 797 GGCGCC G[GA]^CG[CT]C
2834 GGCGCC
KasI 797 GGCGCC G^GCGCC
2834 GGCGCC
KpnI 302 GGTACC GGTAC^C
668 GGTACC
NarI 797 GGCGCC GG^CGCC
2834 GGCGCC
SapI 1494 GAAGAGC GCTCTTC(1/4)
2043 GAAGAGC
SfoI 797 GGCGCC GGC^GCC
2834 GGCGCC
TaqI 2138 TCGA T^CGA
3638 TCGA
TthHB8I 2138 TCGA T^CGA
3638 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI