##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I ApaLI AscI AspI AvrII
BbrPI BbvCI BcgI BfrI BlnI
BsaAI BsaBI BseAI BsiWI BsmBI
BspDI BspEI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI EagI EclXI Eco52I
EcoO65I EcoT22I HincII HindII HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I RsrII SacII SalI
SapI SbfI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 3543 GGTACC G^GTACC
AccII 1275 CGCG CG^CG
AcyI 1416 GGCGCC G[GA]^CG[CT]C
Aor51HI 1925 AGCGCT AGC^GCT
ApaI 1471 GGGCCC GGGCC^C
AseI 3647 ATTAAT AT^TAAT
AsnI 3647 ATTAAT AT^TAAT
Asp700I 438 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3543 GGTACC G^GTACC
BamHI 736 GGATCC G^GATCC
BbeI 1416 GGCGCC GGCGC^C
BciVI 2380 GTATCC GTATCC(6/5)
BlpI 105 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC
BsaHI 1416 GGCGCC G[GA]^CG[CT]C
BspLU11I 1215 ACATGT A^CATGT
BssSI 1208 CACGAG CACGAG(-5/-1)
BstUI 1275 CGCG CG^CG
CelII 105 GCTGAGC GC^T[ACGT]AGC
DraIII 1484 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3455 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1925 AGCGCT AGC^GCT
EcoNI 2474 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3553 GAATTC G^AATTC
EcoRV 1095 GATATC GAT^ATC
FseI 2430 GGCCGGCC GGCCGG^CC
HgaI 2078 GCGTC GACGC(5/10)
Hin1I 1416 GGCGCC G[GA]^CG[CT]C
KasI 1416 GGCGCC G^GCGCC
KpnI 3543 GGTACC GGTAC^C
MvnI 1275 CGCG CG^CG
NaeI 2431 GCCGGC GCC^GGC
NarI 1416 GGCGCC GG^CGCC
NgoMIV 2431 GCCGGC G^CCGGC
PshAI 3816 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3647 ATTAAT AT^TAAT
PvuI 97 CGATCG CGAT^CG
SacI 180 GAGCTC GAGCT^C
ScaI 274 AGTACT AGT^ACT
SfiI 128 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1416 GGCGCC GGC^GCC
StuI 3116 AGGCCT AGG^CCT
XcmI 3586 CCAGCTGTACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 438 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AccI 768 GTCTAC GT^[AC][GT]AC
1146 GTAGAC
AcsI 309 GAATTT [GA]^AATT[CT]
3553 GAATTC
AhdI 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACCGTCAGTC
AocI 1737 CCTAAGG CC^T[ACGT]AGG
2900 CCTCAGG
ApoI 309 GAATTT [GA]^AATT[CT]
3553 GAATTC
AspEI 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACCGTCAGTC
AviII 556 TGCGCA TGC^GCA
1495 TGCGCA
BalI 1121 TGGCCA TGG^CCA
2849 TGGCCA
BclI 187 TGATCA T^GATCA
2698 TGATCA
BglI 129 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
874 GCCACACAGGC
BglII 829 AGATCT A^GATCT
2178 AGATCT
BsaWI 1315 TCCGGT [AT]^CCGG[AT]
1378 TCCGGT
BseRI 82 CTCCTC GAGGAG(10/8)
2878 CTCCTC
BsiEI 97 CGATCG CG[GA][CT]^CG
1277 CGACCG
BsmI 3148 GAATGC GAATGC(1/-1)
2039 GCATTC
Bsp1407I 2400 TGTACA T^GTACA
3755 TGTACA
BspHI 184 TCATGA T^CATGA
3768 TCATGA
BspMI 2620 GCAGGT ACCTGC(4/8)
2839 GCAGGT
BsrFI 1279 ACCGGC [GA]^CCGG[CT]
2431 GCCGGC
BsrGI 2400 TGTACA T^GTACA
3755 TGTACA
Bsu36I 1737 CCTAAGG CC^T[ACGT]AGG
2900 CCTCAGG
Cfr10I 1279 ACCGGC [GA]^CCGG[CT]
2431 GCCGGC
Eam1105I 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2119 GACCGTCAGTC
Eco81I 1737 CCTAAGG CC^T[ACGT]AGG
2900 CCTCAGG
FbaI 187 TGATCA T^GATCA
2698 TGATCA
FspI 556 TGCGCA TGC^GCA
1495 TGCGCA
HaeII 1416 GGCGCC [GA]GCGC^[CT]
1925 AGCGCT
MluNI 1121 TGGCCA TGG^CCA
2849 TGGCCA
MscI 1121 TGGCCA TGG^CCA
2849 TGGCCA
NcoI 142 CCATGG C^CATGG
3089 CCATGG
PflMI 1781 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3558 CCAATTTCTGG
RcaI 184 TCATGA T^CATGA
3768 TCATGA
SmaI 19 CCCGGG CCC^GGG
90 CCCGGG
SspBI 2400 TGTACA T^GTACA
3755 TGTACA
TaqI 178 TCGA T^CGA
3465 TCGA
TfiI 294 GAATC G^A[AT]TC
699 GAATC
TthHB8I 178 TCGA T^CGA
3465 TCGA
Van91I 1781 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3558 CCAATTTCTGG
XmaCI 19 CCCGGG C^CCGGG
90 CCCGGG
XmaI 19 CCCGGG C^CCGGG
90 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII