################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccIII AclI AflII AgeI Alw44I ApaLI AscI AspI AvrII BbrPI BbvCI BcgI BfrI BlnI BsaAI BsaBI BseAI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoO65I EcoT22I HincII HindII HindIII HpaI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI Psp1406I RsrII SacII SalI SapI SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 3543 GGTACC G^GTACC AccII 1275 CGCG CG^CG AcyI 1416 GGCGCC G[GA]^CG[CT]C Aor51HI 1925 AGCGCT AGC^GCT ApaI 1471 GGGCCC GGGCC^C AseI 3647 ATTAAT AT^TAAT AsnI 3647 ATTAAT AT^TAAT Asp700I 438 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 3543 GGTACC G^GTACC BamHI 736 GGATCC G^GATCC BbeI 1416 GGCGCC GGCGC^C BciVI 2380 GTATCC GTATCC(6/5) BlpI 105 GCTGAGC GC^T[ACGT]AGC Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC BsaHI 1416 GGCGCC G[GA]^CG[CT]C BspLU11I 1215 ACATGT A^CATGT BssSI 1208 CACGAG CACGAG(-5/-1) BstUI 1275 CGCG CG^CG CelII 105 GCTGAGC GC^T[ACGT]AGC DraIII 1484 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3455 GACGTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1925 AGCGCT AGC^GCT EcoNI 2474 CCTACTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3553 GAATTC G^AATTC EcoRV 1095 GATATC GAT^ATC FseI 2430 GGCCGGCC GGCCGG^CC HgaI 2078 GCGTC GACGC(5/10) Hin1I 1416 GGCGCC G[GA]^CG[CT]C KasI 1416 GGCGCC G^GCGCC KpnI 3543 GGTACC GGTAC^C MvnI 1275 CGCG CG^CG NaeI 2431 GCCGGC GCC^GGC NarI 1416 GGCGCC GG^CGCC NgoMIV 2431 GCCGGC G^CCGGC PshAI 3816 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3647 ATTAAT AT^TAAT PvuI 97 CGATCG CGAT^CG SacI 180 GAGCTC GAGCT^C ScaI 274 AGTACT AGT^ACT SfiI 128 GGCCGCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1416 GGCGCC GGC^GCC StuI 3116 AGGCCT AGG^CCT XcmI 3586 CCAGCTGTACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 438 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccI 768 GTCTAC GT^[AC][GT]AC 1146 GTAGAC AcsI 309 GAATTT [GA]^AATT[CT] 3553 GAATTC AhdI 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2119 GACCGTCAGTC AocI 1737 CCTAAGG CC^T[ACGT]AGG 2900 CCTCAGG ApoI 309 GAATTT [GA]^AATT[CT] 3553 GAATTC AspEI 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2119 GACCGTCAGTC AviII 556 TGCGCA TGC^GCA 1495 TGCGCA BalI 1121 TGGCCA TGG^CCA 2849 TGGCCA BclI 187 TGATCA T^GATCA 2698 TGATCA BglI 129 GCCGCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 874 GCCACACAGGC BglII 829 AGATCT A^GATCT 2178 AGATCT BsaWI 1315 TCCGGT [AT]^CCGG[AT] 1378 TCCGGT BseRI 82 CTCCTC GAGGAG(10/8) 2878 CTCCTC BsiEI 97 CGATCG CG[GA][CT]^CG 1277 CGACCG BsmI 3148 GAATGC GAATGC(1/-1) 2039 GCATTC Bsp1407I 2400 TGTACA T^GTACA 3755 TGTACA BspHI 184 TCATGA T^CATGA 3768 TCATGA BspMI 2620 GCAGGT ACCTGC(4/8) 2839 GCAGGT BsrFI 1279 ACCGGC [GA]^CCGG[CT] 2431 GCCGGC BsrGI 2400 TGTACA T^GTACA 3755 TGTACA Bsu36I 1737 CCTAAGG CC^T[ACGT]AGG 2900 CCTCAGG Cfr10I 1279 ACCGGC [GA]^CCGG[CT] 2431 GCCGGC Eam1105I 282 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2119 GACCGTCAGTC Eco81I 1737 CCTAAGG CC^T[ACGT]AGG 2900 CCTCAGG FbaI 187 TGATCA T^GATCA 2698 TGATCA FspI 556 TGCGCA TGC^GCA 1495 TGCGCA HaeII 1416 GGCGCC [GA]GCGC^[CT] 1925 AGCGCT MluNI 1121 TGGCCA TGG^CCA 2849 TGGCCA MscI 1121 TGGCCA TGG^CCA 2849 TGGCCA NcoI 142 CCATGG C^CATGG 3089 CCATGG PflMI 1781 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3558 CCAATTTCTGG RcaI 184 TCATGA T^CATGA 3768 TCATGA SmaI 19 CCCGGG CCC^GGG 90 CCCGGG SspBI 2400 TGTACA T^GTACA 3755 TGTACA TaqI 178 TCGA T^CGA 3465 TCGA TfiI 294 GAATC G^A[AT]TC 699 GAATC TthHB8I 178 TCGA T^CGA 3465 TCGA Van91I 1781 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3558 CCAATTTCTGG XmaCI 19 CCCGGG C^CCGGG 90 CCCGGG XmaI 19 CCCGGG C^CCGGG 90 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI XhoII