##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MslI NdeI NheI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2372 TCCGGA T^CCGGA
AflIII 3417 ACGCGT A^C[GA][CT]GT
AhdI 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2970 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1060 AGCGCT AGC^GCT
Asp700I 3559 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3645 TGGCCA TGG^CCA
BsaWI 2372 TCCGGA [AT]^CCGG[AT]
BseAI 2372 TCCGGA T^CCGGA
BsmBI 2883 GAGACG CGTCTC(1/5)
BsmI 3559 GAATGC GAATGC(1/-1)
BspEI 2372 TCCGGA T^CCGGA
BsrBI 1 CCGCTC CCGCTC(-3/-3)
BsrDI 1094 CATTGC GCAATG(2/0)
BstF5I 3008 GGATG GGATG(2/0)
Bsu36I 2970 CCTGAGG CC^T[ACGT]AGG
DrdI 2951 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1060 AGCGCT AGC^GCT
Eco81I 2970 CCTGAGG CC^T[ACGT]AGG
EcoRV 2268 GATATC GAT^ATC
FokI 3008 GGATG GGATG(9/13)
MluI 3417 ACGCGT A^CGCGT
MluNI 3645 TGGCCA TGG^CCA
MroI 2372 TCCGGA T^CCGGA
MscI 3645 TGGCCA TGG^CCA
NcoI 448 CCATGG C^CATGG
NotI 12 GCGGCCGC GC^GGCCGC
NruI 803 TCGCGA TCG^CGA
PpuMI 1183 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2192 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 197 GAGCTC GAGCT^C
SapI 3616 GAAGAGC GCTCTTC(1/4)
ScaI 3193 AGTACT AGT^ACT
SexAI 1907 ACCAGGT A^CC[AT]GGT
SmaI 131 CCCGGG CCC^GGG
XbaI 2357 TCTAGA T^CTAGA
XmaCI 131 CCCGGG C^CCGGG
XmaI 131 CCCGGG C^CCGGG
XmnI 3559 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 188 GACGTC GACGT^C
827 GACGTC
BssSI 176 CTCGTG CACGAG(-5/-1)
1576 CTCGTG
BstAPI 686 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1089 GCAGACATTGC
BstXI 1012 CCACAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3576 CCAAAGGAGTGG
EcoNI 530 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
545 CCTGCTGGAGG
HgaI 807 GACGC GACGC(5/10)
1110 GACGC
KspI 148 CCGCGG CCGC^GG
227 CCGCGG
MfeI 2161 CAATTG C^AATTG
3692 CAATTG
MseI 3296 TTAA T^TAA
3302 TTAA
MunI 2161 CAATTG C^AATTG
3692 CAATTG
NaeI 153 GCCGGC GCC^GGC
435 GCCGGC
NgoMIV 153 GCCGGC G^CCGGC
435 GCCGGC
PaeR7I 239 CTCGAG C^TCGAG
378 CTCGAG
PflMI 572 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3358 CCAGGTGATGG
SacII 148 CCGCGG CCGC^GG
227 CCGCGG
Tru9I 3296 TTAA T^TAA
3302 TTAA
Van91I 572 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3358 CCAGGTGATGG
XcmI 1039 CCAAGGAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1147 CCAAGTGGGAGATGG
XhoI 239 CTCGAG C^TCGAG
378 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII