################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EcoO65I EcoRI EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MamI MslI NdeI NheI NsiI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2372 TCCGGA T^CCGGA AflIII 3417 ACGCGT A^C[GA][CT]GT AhdI 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2970 CCTGAGG CC^T[ACGT]AGG Aor51HI 1060 AGCGCT AGC^GCT Asp700I 3559 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 3645 TGGCCA TGG^CCA BsaWI 2372 TCCGGA [AT]^CCGG[AT] BseAI 2372 TCCGGA T^CCGGA BsmBI 2883 GAGACG CGTCTC(1/5) BsmI 3559 GAATGC GAATGC(1/-1) BspEI 2372 TCCGGA T^CCGGA BsrBI 1 CCGCTC CCGCTC(-3/-3) BsrDI 1094 CATTGC GCAATG(2/0) BstF5I 3008 GGATG GGATG(2/0) Bsu36I 2970 CCTGAGG CC^T[ACGT]AGG DrdI 2951 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 822 GACGTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1060 AGCGCT AGC^GCT Eco81I 2970 CCTGAGG CC^T[ACGT]AGG EcoRV 2268 GATATC GAT^ATC FokI 3008 GGATG GGATG(9/13) MluI 3417 ACGCGT A^CGCGT MluNI 3645 TGGCCA TGG^CCA MroI 2372 TCCGGA T^CCGGA MscI 3645 TGGCCA TGG^CCA NcoI 448 CCATGG C^CATGG NotI 12 GCGGCCGC GC^GGCCGC NruI 803 TCGCGA TCG^CGA PpuMI 1183 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2192 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 197 GAGCTC GAGCT^C SapI 3616 GAAGAGC GCTCTTC(1/4) ScaI 3193 AGTACT AGT^ACT SexAI 1907 ACCAGGT A^CC[AT]GGT SmaI 131 CCCGGG CCC^GGG XbaI 2357 TCTAGA T^CTAGA XmaCI 131 CCCGGG C^CCGGG XmaI 131 CCCGGG C^CCGGG XmnI 3559 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AatII 188 GACGTC GACGT^C 827 GACGTC BssSI 176 CTCGTG CACGAG(-5/-1) 1576 CTCGTG BstAPI 686 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1089 GCAGACATTGC BstXI 1012 CCACAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3576 CCAAAGGAGTGG EcoNI 530 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 545 CCTGCTGGAGG HgaI 807 GACGC GACGC(5/10) 1110 GACGC KspI 148 CCGCGG CCGC^GG 227 CCGCGG MfeI 2161 CAATTG C^AATTG 3692 CAATTG MseI 3296 TTAA T^TAA 3302 TTAA MunI 2161 CAATTG C^AATTG 3692 CAATTG NaeI 153 GCCGGC GCC^GGC 435 GCCGGC NgoMIV 153 GCCGGC G^CCGGC 435 GCCGGC PaeR7I 239 CTCGAG C^TCGAG 378 CTCGAG PflMI 572 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3358 CCAGGTGATGG SacII 148 CCGCGG CCGC^GG 227 CCGCGG Tru9I 3296 TTAA T^TAA 3302 TTAA Van91I 572 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3358 CCAGGTGATGG XcmI 1039 CCAAGGAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1147 CCAAGTGGGAGATGG XhoI 239 CTCGAG C^TCGAG 378 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII