##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

Acc65I      AccI        AclI        AflII       AgeI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AviII       AvrII       
BamHI       BbrPI       BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsaBI       
BsaI        BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DraI        
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        MamI        MslI        NdeI        NheI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SbfI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII       2372  TCCGGA               T^CCGGA
AflIII       3417  ACGCGT               A^C[GA][CT]GT
AhdI          822  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2970  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1060  AGCGCT               AGC^GCT
Asp700I      3559  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         822  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         3645  TGGCCA               TGG^CCA
BsaWI        2372  TCCGGA               [AT]^CCGG[AT]
BseAI        2372  TCCGGA               T^CCGGA
BsmBI        2883  GAGACG               CGTCTC(1/5)
BsmI         3559  GAATGC               GAATGC(1/-1)
BspEI        2372  TCCGGA               T^CCGGA
BsrBI           1  CCGCTC               CCGCTC(-3/-3)
BsrDI        1094  CATTGC               GCAATG(2/0)
BstF5I       3008  GGATG                GGATG(2/0)
Bsu36I       2970  CCTGAGG              CC^T[ACGT]AGG
DrdI         2951  GACCACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      822  GACGTGACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1060  AGCGCT               AGC^GCT
Eco81I       2970  CCTGAGG              CC^T[ACGT]AGG
EcoRV        2268  GATATC               GAT^ATC
FokI         3008  GGATG                GGATG(9/13)
MluI         3417  ACGCGT               A^CGCGT
MluNI        3645  TGGCCA               TGG^CCA
MroI         2372  TCCGGA               T^CCGGA
MscI         3645  TGGCCA               TGG^CCA
NcoI          448  CCATGG               C^CATGG
NotI           12  GCGGCCGC             GC^GGCCGC
NruI          803  TCGCGA               TCG^CGA
PpuMI        1183  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI        2192  GACCCCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI          197  GAGCTC               GAGCT^C
SapI         3616  GAAGAGC              GCTCTTC(1/4)
ScaI         3193  AGTACT               AGT^ACT
SexAI        1907  ACCAGGT              A^CC[AT]GGT
SmaI          131  CCCGGG               CCC^GGG
XbaI         2357  TCTAGA               T^CTAGA
XmaCI         131  CCCGGG               C^CCGGG
XmaI          131  CCCGGG               C^CCGGG
XmnI         3559  GAATGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AatII         188  GACGTC               GACGT^C
              827  GACGTC              
BssSI         176  CTCGTG               CACGAG(-5/-1)
             1576  CTCGTG              
BstAPI        686  GCAGGCGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1089  GCAGACATTGC         
BstXI        1012  CCACAGAGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3576  CCAAAGGAGTGG        
EcoNI         530  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              545  CCTGCTGGAGG         
HgaI          807  GACGC                GACGC(5/10)
             1110  GACGC               
KspI          148  CCGCGG               CCGC^GG
              227  CCGCGG              
MfeI         2161  CAATTG               C^AATTG
             3692  CAATTG              
MseI         3296  TTAA                 T^TAA
             3302  TTAA                
MunI         2161  CAATTG               C^AATTG
             3692  CAATTG              
NaeI          153  GCCGGC               GCC^GGC
              435  GCCGGC              
NgoMIV        153  GCCGGC               G^CCGGC
              435  GCCGGC              
PaeR7I        239  CTCGAG               C^TCGAG
              378  CTCGAG              
PflMI         572  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3358  CCAGGTGATGG         
SacII         148  CCGCGG               CCGC^GG
              227  CCGCGG              
Tru9I        3296  TTAA                 T^TAA
             3302  TTAA                
Van91I        572  CCAGCGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3358  CCAGGTGATGG         
XcmI         1039  CCAAGGAGTCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1147  CCAAGTGGGAGATGG     
XhoI          239  CTCGAG               C^TCGAG
              378  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  114 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AspHI       
AvaI        AvaII       BanI        BanII       BbeI        
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaHI       BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssHII      BssKI       BstNI       BstUI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SfoI        
SmlI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII