##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AspI AviII
BbsI BcgI BciVI BclI BfrI
BglI BpuAI BsaBI BseAI BsiWI
BspDI BspEI BspLU11I BsrFI BssHII
BssSI BstBI Cfr10I ClaI CpoI
DraI Eam1105I Eco47III EcoRV FbaI
FseI FspI HindIII HpaI KspI
MamI MluI MroI MseI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Tru9I Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 432 GGTACC G^GTACC
AccI 1593 GTATAC GT^[AC][GT]AC
AccII 2836 CGCG CG^CG
AflIII 702 ACGTGT A^C[GA][CT]GT
Alw44I 1210 GTGCAC G^TGCAC
ApaLI 1210 GTGCAC G^TGCAC
Asp718I 432 GGTACC G^GTACC
AvaI 2191 CCCGAG C^[CT]CG[GA]G
BbeI 3082 GGCGCC GGCGC^C
BbrPI 1912 CACGTG CAC^GTG
BbvCI 904 GCTGAGG CCTCAGC(-5/-2)
BlpI 196 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 196 GCTTAGC GC^T[ACGT]AGC
BsaWI 2726 ACCGGA [AT]^CCGG[AT]
BsmI 3441 GAATGC GAATGC(1/-1)
BsoBI 2191 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2313 TGTACA T^GTACA
BspHI 1673 TCATGA T^CATGA
BsrBI 890 GAGCGG CCGCTC(-3/-3)
BsrDI 2448 CATTGC GCAATG(2/0)
BsrGI 2313 TGTACA T^GTACA
Bst1107I 1593 GTATAC GTA^TAC
BstEII 2677 GGTCACC G^GT[ACGT]ACC
BstPI 2677 GGTCACC G^GT[ACGT]ACC
BstUI 2836 CGCG CG^CG
BstZ17I 1593 GTATAC GTA^TAC
CelII 196 GCTTAGC GC^T[ACGT]AGC
DraIII 2242 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 331 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2568 CGGCCG C^GGCCG
EclXI 2568 CGGCCG C^GGCCG
Eco52I 2568 CGGCCG C^GGCCG
EcoO65I 2677 GGTCACC G^GT[ACGT]ACC
EcoRI 2587 GAATTC G^AATTC
EcoT22I 1850 ATGCAT ATGCA^T
HaeII 3082 GGCGCC [GA]GCGC^[CT]
KasI 3082 GGCGCC G^GCGCC
KpnI 432 GGTACC GGTAC^C
MfeI 3446 CAATTG C^AATTG
MunI 3446 CAATTG C^AATTG
MvnI 2836 CGCG CG^CG
NarI 3082 GGCGCC GG^CGCC
NsiI 1850 ATGCAT ATGCA^T
PmaCI 1912 CACGTG CAC^GTG
PmlI 1912 CACGTG CAC^GTG
PshAI 1633 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1673 TCATGA T^CATGA
SacI 3188 GAGCTC GAGCT^C
SapI 3190 GCTCTTC GCTCTTC(1/4)
SexAI 2243 ACCAGGT A^CC[AT]GGT
SfoI 3082 GGCGCC GGC^GCC
SspBI 2313 TGTACA T^GTACA
TfiI 915 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 2539 AAATTC [GA]^AATT[CT]
2587 GAATTC
ApoI 2539 AAATTC [GA]^AATT[CT]
2587 GAATTC
AvrII 3535 CCTAGG C^CTAGG
3882 CCTAGG
BglII 401 AGATCT A^GATCT
746 AGATCT
BlnI 3535 CCTAGG C^CTAGG
3882 CCTAGG
BsaAI 701 TACGTG [CT]AC^GT[GA]
1912 CACGTG
BsiEI 1095 CGACCG CG[GA][CT]^CG
2568 CGGCCG
BsmBI 2838 CGTCTC CGTCTC(1/5)
3045 GAGACG
EcoNI 453 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2487 CCTGAGGCAGG
HincII 2602 GTCAAC GT[CT]^[GA]AC
3418 GTCAAC
HindII 2602 GTCAAC GT[CT]^[GA]AC
3418 GTCAAC
SmlI 157 CTTGAG C^T[CT][GA]AG
1489 CTTGAG
StuI 2412 AGGCCT AGG^CCT
3106 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AocI AspHI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII