##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
BbsI        BcgI        BciVI       BclI        BfrI        
BglI        BpuAI       BsaBI       BseAI       BsiWI       
BspDI       BspEI       BspLU11I    BsrFI       BssHII      
BssSI       BstBI       Cfr10I      ClaI        CpoI        
DraI        Eam1105I    Eco47III    EcoRV       FbaI        
FseI        FspI        HindIII     HpaI        KspI        
MamI        MluI        MroI        MseI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tru9I       Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I        432  GGTACC               G^GTACC
AccI         1593  GTATAC               GT^[AC][GT]AC
AccII        2836  CGCG                 CG^CG
AflIII        702  ACGTGT               A^C[GA][CT]GT
Alw44I       1210  GTGCAC               G^TGCAC
ApaLI        1210  GTGCAC               G^TGCAC
Asp718I       432  GGTACC               G^GTACC
AvaI         2191  CCCGAG               C^[CT]CG[GA]G
BbeI         3082  GGCGCC               GGCGC^C
BbrPI        1912  CACGTG               CAC^GTG
BbvCI         904  GCTGAGG              CCTCAGC(-5/-2)
BlpI          196  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      196  GCTTAGC              GC^T[ACGT]AGC
BsaWI        2726  ACCGGA               [AT]^CCGG[AT]
BsmI         3441  GAATGC               GAATGC(1/-1)
BsoBI        2191  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2313  TGTACA               T^GTACA
BspHI        1673  TCATGA               T^CATGA
BsrBI         890  GAGCGG               CCGCTC(-3/-3)
BsrDI        2448  CATTGC               GCAATG(2/0)
BsrGI        2313  TGTACA               T^GTACA
Bst1107I     1593  GTATAC               GTA^TAC
BstEII       2677  GGTCACC              G^GT[ACGT]ACC
BstPI        2677  GGTCACC              G^GT[ACGT]ACC
BstUI        2836  CGCG                 CG^CG
BstZ17I      1593  GTATAC               GTA^TAC
CelII         196  GCTTAGC              GC^T[ACGT]AGC
DraIII       2242  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          331  GACAACACGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2568  CGGCCG               C^GGCCG
EclXI        2568  CGGCCG               C^GGCCG
Eco52I       2568  CGGCCG               C^GGCCG
EcoO65I      2677  GGTCACC              G^GT[ACGT]ACC
EcoRI        2587  GAATTC               G^AATTC
EcoT22I      1850  ATGCAT               ATGCA^T
HaeII        3082  GGCGCC               [GA]GCGC^[CT]
KasI         3082  GGCGCC               G^GCGCC
KpnI          432  GGTACC               GGTAC^C
MfeI         3446  CAATTG               C^AATTG
MunI         3446  CAATTG               C^AATTG
MvnI         2836  CGCG                 CG^CG
NarI         3082  GGCGCC               GG^CGCC
NsiI         1850  ATGCAT               ATGCA^T
PmaCI        1912  CACGTG               CAC^GTG
PmlI         1912  CACGTG               CAC^GTG
PshAI        1633  GACAATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         1673  TCATGA               T^CATGA
SacI         3188  GAGCTC               GAGCT^C
SapI         3190  GCTCTTC              GCTCTTC(1/4)
SexAI        2243  ACCAGGT              A^CC[AT]GGT
SfoI         3082  GGCGCC               GGC^GCC
SspBI        2313  TGTACA               T^GTACA
TfiI          915  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI         2539  AAATTC               [GA]^AATT[CT]
             2587  GAATTC              
ApoI         2539  AAATTC               [GA]^AATT[CT]
             2587  GAATTC              
AvrII        3535  CCTAGG               C^CTAGG
             3882  CCTAGG              
BglII         401  AGATCT               A^GATCT
              746  AGATCT              
BlnI         3535  CCTAGG               C^CTAGG
             3882  CCTAGG              
BsaAI         701  TACGTG               [CT]AC^GT[GA]
             1912  CACGTG              
BsiEI        1095  CGACCG               CG[GA][CT]^CG
             2568  CGGCCG              
BsmBI        2838  CGTCTC               CGTCTC(1/5)
             3045  GAGACG              
EcoNI         453  CCTGGACAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2487  CCTGAGGCAGG         
HincII       2602  GTCAAC               GT[CT]^[GA]AC
             3418  GTCAAC              
HindII       2602  GTCAAC               GT[CT]^[GA]AC
             3418  GTCAAC              
SmlI          157  CTTGAG               C^T[CT][GA]AG
             1489  CTTGAG              
StuI         2412  AGGCCT               AGG^CCT
             3106  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AocI        AspHI       AvaII       BalI        
BamHI       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoII