################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII BbsI BcgI BciVI BclI BfrI BglI BpuAI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BsrFI BssHII BssSI BstBI Cfr10I ClaI CpoI DraI Eam1105I Eco47III EcoRV FbaI FseI FspI HindIII HpaI KspI MamI MluI MroI MseI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 432 GGTACC G^GTACC AccI 1593 GTATAC GT^[AC][GT]AC AccII 2836 CGCG CG^CG AflIII 702 ACGTGT A^C[GA][CT]GT Alw44I 1210 GTGCAC G^TGCAC ApaLI 1210 GTGCAC G^TGCAC Asp718I 432 GGTACC G^GTACC AvaI 2191 CCCGAG C^[CT]CG[GA]G BbeI 3082 GGCGCC GGCGC^C BbrPI 1912 CACGTG CAC^GTG BbvCI 904 GCTGAGG CCTCAGC(-5/-2) BlpI 196 GCTTAGC GC^T[ACGT]AGC Bpu1102I 196 GCTTAGC GC^T[ACGT]AGC BsaWI 2726 ACCGGA [AT]^CCGG[AT] BsmI 3441 GAATGC GAATGC(1/-1) BsoBI 2191 CCCGAG C^[CT]CG[GA]G Bsp1407I 2313 TGTACA T^GTACA BspHI 1673 TCATGA T^CATGA BsrBI 890 GAGCGG CCGCTC(-3/-3) BsrDI 2448 CATTGC GCAATG(2/0) BsrGI 2313 TGTACA T^GTACA Bst1107I 1593 GTATAC GTA^TAC BstEII 2677 GGTCACC G^GT[ACGT]ACC BstPI 2677 GGTCACC G^GT[ACGT]ACC BstUI 2836 CGCG CG^CG BstZ17I 1593 GTATAC GTA^TAC CelII 196 GCTTAGC GC^T[ACGT]AGC DraIII 2242 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 331 GACAACACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2568 CGGCCG C^GGCCG EclXI 2568 CGGCCG C^GGCCG Eco52I 2568 CGGCCG C^GGCCG EcoO65I 2677 GGTCACC G^GT[ACGT]ACC EcoRI 2587 GAATTC G^AATTC EcoT22I 1850 ATGCAT ATGCA^T HaeII 3082 GGCGCC [GA]GCGC^[CT] KasI 3082 GGCGCC G^GCGCC KpnI 432 GGTACC GGTAC^C MfeI 3446 CAATTG C^AATTG MunI 3446 CAATTG C^AATTG MvnI 2836 CGCG CG^CG NarI 3082 GGCGCC GG^CGCC NsiI 1850 ATGCAT ATGCA^T PmaCI 1912 CACGTG CAC^GTG PmlI 1912 CACGTG CAC^GTG PshAI 1633 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1673 TCATGA T^CATGA SacI 3188 GAGCTC GAGCT^C SapI 3190 GCTCTTC GCTCTTC(1/4) SexAI 2243 ACCAGGT A^CC[AT]GGT SfoI 3082 GGCGCC GGC^GCC SspBI 2313 TGTACA T^GTACA TfiI 915 GATTC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 2539 AAATTC [GA]^AATT[CT] 2587 GAATTC ApoI 2539 AAATTC [GA]^AATT[CT] 2587 GAATTC AvrII 3535 CCTAGG C^CTAGG 3882 CCTAGG BglII 401 AGATCT A^GATCT 746 AGATCT BlnI 3535 CCTAGG C^CTAGG 3882 CCTAGG BsaAI 701 TACGTG [CT]AC^GT[GA] 1912 CACGTG BsiEI 1095 CGACCG CG[GA][CT]^CG 2568 CGGCCG BsmBI 2838 CGTCTC CGTCTC(1/5) 3045 GAGACG EcoNI 453 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2487 CCTGAGGCAGG HincII 2602 GTCAAC GT[CT]^[GA]AC 3418 GTCAAC HindII 2602 GTCAAC GT[CT]^[GA]AC 3418 GTCAAC SmlI 157 CTTGAG C^T[CT][GA]AG 1489 CTTGAG StuI 2412 AGGCCT AGG^CCT 3106 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII