##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AspEI AviII AvrII
BcgI BclI BfrI BlnI BsaBI
BsiWI BsmI BspDI Bst1107I BstBI
BstZ17I ClaI CpoI Eam1105I Eco47III
EcoRV FbaI FseI FspI HindIII
HpaI MamI MfeI MunI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 2376 GGTACC G^GTACC
AccI 2657 GTAGAC GT^[AC][GT]AC
AccIII 1263 TCCGGA T^CCGGA
AocI 1685 CCTGAGG CC^T[ACGT]AGG
ApaI 2595 GGGCCC GGGCC^C
AseI 4441 ATTAAT AT^TAAT
AsnI 4441 ATTAAT AT^TAAT
Asp700I 1294 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2376 GGTACC G^GTACC
AspI 271 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 602 TGGCCA TGG^CCA
BbeI 3326 GGCGCC GGCGC^C
BbvCI 2817 CCTCAGC CCTCAGC(-5/-2)
BciVI 494 GGATAC GTATCC(6/5)
BseAI 1263 TCCGGA T^CCGGA
BsmBI 525 CGTCTC CGTCTC(1/5)
Bsp1407I 4452 TGTACA T^GTACA
BspEI 1263 TCCGGA T^CCGGA
BspHI 1255 TCATGA T^CATGA
BsrBI 3601 CCGCTC CCGCTC(-3/-3)
BsrGI 4452 TGTACA T^GTACA
BssSI 764 CACGAG CACGAG(-5/-1)
Bsu36I 1685 CCTGAGG CC^T[ACGT]AGG
DrdI 3449 GACGTGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1685 CCTGAGG CC^T[ACGT]AGG
EcoRI 2617 GAATTC G^AATTC
EcoT22I 4487 ATGCAT ATGCA^T
HincII 2759 GTCAAC GT[CT]^[GA]AC
HindII 2759 GTCAAC GT[CT]^[GA]AC
KasI 3326 GGCGCC G^GCGCC
KpnI 2376 GGTACC GGTAC^C
KspI 3846 CCGCGG CCGC^GG
MluI 2913 ACGCGT A^CGCGT
MluNI 602 TGGCCA TGG^CCA
MroI 1263 TCCGGA T^CCGGA
MscI 602 TGGCCA TGG^CCA
NarI 3326 GGCGCC GG^CGCC
NdeI 3527 CATATG CA^TATG
NsiI 4487 ATGCAT ATGCA^T
PflFI 271 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4441 ATTAAT AT^TAAT
PvuII 139 CAGCTG CAG^CTG
RcaI 1255 TCATGA T^CATGA
SacII 3846 CCGCGG CCGC^GG
SapI 3137 GAAGAGC GCTCTTC(1/4)
SbfI 3703 CCTGCAGG CCTGCA^GG
ScaI 3945 AGTACT AGT^ACT
SfoI 3326 GGCGCC GGC^GCC
SmlI 1041 CTCAAG C^T[CT][GA]AG
Sse8387I 3703 CCTGCAGG CCTGCA^GG
SspBI 4452 TGTACA T^GTACA
Tth111I 271 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 1294 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 3288 CTCGGG C^[CT]CG[GA]G
3968 CCCGAG
BamHI 749 GGATCC G^GATCC
3559 GGATCC
BbrPI 88 CACGTG CAC^GTG
723 CACGTG
BglII 107 AGATCT A^GATCT
993 AGATCT
BsiEI 3774 CGGCCG CG[GA][CT]^CG
3840 CGGCCG
BsoBI 3288 CTCGGG C^[CT]CG[GA]G
3968 CCCGAG
BsrDI 1976 CATTGC GCAATG(2/0)
2576 CATTGC
BstAPI 3028 GCACCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3706 GCAGGAGGTGC
BstEII 268 GGTGACC G^GT[ACGT]ACC
2849 GGTCACC
BstPI 268 GGTGACC G^GT[ACGT]ACC
2849 GGTCACC
EagI 3774 CGGCCG C^GGCCG
3840 CGGCCG
EclXI 3774 CGGCCG C^GGCCG
3840 CGGCCG
Eco52I 3774 CGGCCG C^GGCCG
3840 CGGCCG
EcoNI 1742 CCTTTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3703 CCTGCAGGAGG
EcoO65I 268 GGTGACC G^GT[ACGT]ACC
2849 GGTCACC
PmaCI 88 CACGTG CAC^GTG
723 CACGTG
PmlI 88 CACGTG CAC^GTG
723 CACGTG
SexAI 272 ACCTGGT A^CC[AT]GGT
2763 ACCTGGT
StuI 2890 AGGCCT AGG^CCT
3397 AGGCCT
TfiI 2088 GAATC G^A[AT]TC
3749 GATTC
XbaI 996 TCTAGA T^CTAGA
2725 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII