##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccIII AgeI Alw44I ApaI
ApaLI AscI Asp718I AspI AviII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BglI BpuAI
BsaAI BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssSI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EcoRV FbaI FseI FspI HpaI
KasI KpnI MamI MluNI MroI
MscI NaeI NarI NdeI NgoMIV
NheI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1651 GACGTC GACGT^C
AccI 745 GTCGAC GT^[AC][GT]AC
AclI 1203 AACGTT AA^CGTT
AflII 2104 CTTAAG C^TTAAG
AhdI 1310 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3360 CCTGAGG CC^T[ACGT]AGG
Aor51HI 4127 AGCGCT AGC^GCT
Asp700I 2848 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1310 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2832 CCTAGG C^CTAGG
BanII 1251 GAGCTC G[GA]GC[CT]^C
BfrI 2104 CTTAAG C^TTAAG
BlnI 2832 CCTAGG C^CTAGG
BsaWI 864 TCCGGT [AT]^CCGG[AT]
BspHI 2778 TCATGA T^CATGA
BsrBI 1642 GAGCGG CCGCTC(-3/-3)
BsrFI 138 GCCGGT [GA]^CCGG[CT]
BssHII 53 GCGCGC G^CGCGC
BstAPI 1989 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 607 GGTGACC G^GT[ACGT]ACC
BstPI 607 GGTGACC G^GT[ACGT]ACC
Bsu36I 3360 CCTGAGG CC^T[ACGT]AGG
Cfr10I 138 GCCGGT [GA]^CCGG[CT]
DraI 4211 TTTAAA TTT^AAA
Eam1105I 1310 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4127 AGCGCT AGC^GCT
Eco81I 3360 CCTGAGG CC^T[ACGT]AGG
EcoNI 3052 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 607 GGTGACC G^GT[ACGT]ACC
EcoRI 914 GAATTC G^AATTC
EcoT22I 3921 ATGCAT ATGCA^T
HaeII 4127 AGCGCT [GA]GCGC^[CT]
HincII 745 GTCGAC GT[CT]^[GA]AC
HindII 745 GTCGAC GT[CT]^[GA]AC
HindIII 1420 AAGCTT A^AGCTT
KspI 40 CCGCGG CCGC^GG
MfeI 3095 CAATTG C^AATTG
MluI 2699 ACGCGT A^CGCGT
MunI 3095 CAATTG C^AATTG
NotI 36 GCGGCCGC GC^GGCCGC
NsiI 3921 ATGCAT ATGCA^T
Psp1406I 1203 AACGTT AA^CGTT
PvuII 1242 CAGCTG CAG^CTG
RcaI 2778 TCATGA T^CATGA
SacI 1251 GAGCTC GAGCT^C
SacII 40 CCGCGG CCGC^GG
SalI 745 GTCGAC G^TCGAC
SphI 1055 GCATGC GCATG^C
XcmI 3182 CCAATAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2848 GAATCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 914 GAATTC [GA]^AATT[CT]
2838 AAATTT
ApoI 914 GAATTC [GA]^AATT[CT]
2838 AAATTT
AseI 4011 ATTAAT AT^TAAT
4277 ATTAAT
AsnI 4011 ATTAAT AT^TAAT
4277 ATTAAT
AvaI 63 CTCGGG C^[CT]CG[GA]G
1063 CCCGAG
BanI 1023 GGCACC G^G[CT][GA]CC
1131 GGCACC
BlpI 1333 GCTCAGC GC^T[ACGT]AGC
4130 GCTGAGC
Bpu1102I 1333 GCTCAGC GC^T[ACGT]AGC
4130 GCTGAGC
BsaI 3667 GAGACC GGTCTC(1/5)
258 GGTCTC
BsiEI 37 CGGCCG CG[GA][CT]^CG
2900 CGGCCG
BsmBI 21 CGTCTC CGTCTC(1/5)
29 CGTCTC
BsoBI 63 CTCGGG C^[CT]CG[GA]G
1063 CCCGAG
BspLU11I 2185 ACATGT A^CATGT
2537 ACATGT
BspMI 749 ACCTGC ACCTGC(4/8)
4138 ACCTGC
CelII 1333 GCTCAGC GC^T[ACGT]AGC
4130 GCTGAGC
DraIII 85 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
811 CACAATGTG
EagI 37 CGGCCG C^GGCCG
2900 CGGCCG
EclXI 37 CGGCCG C^GGCCG
2900 CGGCCG
Eco52I 37 CGGCCG C^GGCCG
2900 CGGCCG
PflMI 1495 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1618 CCACTGATTGG
PshBI 4011 ATTAAT AT^TAAT
4277 ATTAAT
Van91I 1495 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1618 CCACTGATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AspHI AvaII
BbvI BcgI BcnI BfaI BglII
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII