##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AgeI AhdI ApaI AscI Asp718I
AspEI AspI AviII AvrII BcgI
BciVI BcnI BfrI BlnI BsiWI
BsmBI BspDI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
ClaI CpoI DrdI Eam1105I EcoO65I
EcoRI EcoRV FauI FspI HpaI
KpnI KspI MfeI MluI MunI
MvnI NciI NcoI NdeI NotI
NruI PacI PaeR7I PflFI PinAI
PshAI Psp1406I RsrII SacII SalI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 52 TCCGGA T^CCGGA
AcyI 2920 GGCGCC G[GA]^CG[CT]C
AseI 2420 ATTAAT AT^TAAT
AsnI 2420 ATTAAT AT^TAAT
BalI 1764 TGGCCA TGG^CCA
BamHI 511 GGATCC G^GATCC
BbeI 2920 GGCGCC GGCGC^C
BbrPI 634 CACGTG CAC^GTG
BclI 925 TGATCA T^GATCA
BsaAI 634 CACGTG [CT]AC^GT[GA]
BsaHI 2920 GGCGCC G[GA]^CG[CT]C
BseAI 52 TCCGGA T^CCGGA
BsgI 575 GTGCAG GTGCAG(16/14)
BspEI 52 TCCGGA T^CCGGA
BspHI 811 TCATGA T^CATGA
BstBI 1327 TTCGAA TT^CGAA
DsaI 183 CCACGG C^C[GA][CT]GG
EagI 3642 CGGCCG C^GGCCG
EclXI 3642 CGGCCG C^GGCCG
Eco52I 3642 CGGCCG C^GGCCG
EcoNI 3267 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 925 TGATCA T^GATCA
FseI 3639 GGCCGGCC GGCCGG^CC
Hin1I 2920 GGCGCC G[GA]^CG[CT]C
HincII 3392 GTTGAC GT[CT]^[GA]AC
HindII 3392 GTTGAC GT[CT]^[GA]AC
KasI 2920 GGCGCC G^GCGCC
MaeII 635 ACGT A^CGT
MluNI 1764 TGGCCA TGG^CCA
MroI 52 TCCGGA T^CCGGA
MscI 1764 TGGCCA TGG^CCA
NarI 2920 GGCGCC GG^CGCC
NheI 4040 GCTAGC G^CTAGC
NspV 1327 TTCGAA TT^CGAA
PflMI 2430 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 634 CACGTG CAC^GTG
PmeI 1051 GTTTAAAC GTTT^AAAC
PmlI 634 CACGTG CAC^GTG
PpuMI 1399 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2420 ATTAAT AT^TAAT
PvuI 827 CGATCG CGAT^CG
RcaI 811 TCATGA T^CATGA
SacI 281 GAGCTC GAGCT^C
SbfI 1403 CCTGCAGG CCTGCA^GG
SfoI 2920 GGCGCC GGC^GCC
SfuI 1327 TTCGAA TT^CGAA
SphI 1143 GCATGC GCATG^C
Sse8387I 1403 CCTGCAGG CCTGCA^GG
SwaI 1029 ATTTAAAT ATTT^AAAT
Van91I 2430 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccI 1691 GTAGAC GT^[AC][GT]AC
4005 GTAGAC
Alw44I 3131 GTGCAC G^TGCAC
3241 GTGCAC
AlwNI 993 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
2431 CAGCAACTG
AocI 2970 CCTGAGG CC^T[ACGT]AGG
3508 CCTTAGG
Aor51HI 1113 AGCGCT AGC^GCT
1748 AGCGCT
ApaLI 3131 GTGCAC G^TGCAC
3241 GTGCAC
Asp700I 1628 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3423 GAAGGAGTTC
BanII 281 GAGCTC G[GA]GC[CT]^C
1567 GGGCTC
BbvCI 2332 CCTCAGC CCTCAGC(-5/-2)
1166 GCTGAGG
BglI 3014 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3095 GCCCAGTGGGC
BglII 2904 AGATCT A^GATCT
3839 AGATCT
BlpI 3560 GCTCAGC GC^T[ACGT]AGC
3749 GCTTAGC
Bpu1102I 3560 GCTCAGC GC^T[ACGT]AGC
3749 GCTTAGC
BsaBI 2149 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2681 GATATTCATC
BsmI 3895 GAATGC GAATGC(1/-1)
301 GCATTC
BsrDI 4027 CATTGC GCAATG(2/0)
1737 GCAATG
BsrFI 3640 GCCGGC [GA]^CCGG[CT]
3644 GCCGGC
BstXI 1345 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1575 CCACCTCTGTGG
Bsu36I 2970 CCTGAGG CC^T[ACGT]AGG
3508 CCTTAGG
CelII 3560 GCTCAGC GC^T[ACGT]AGC
3749 GCTTAGC
Cfr10I 3640 GCCGGC [GA]^CCGG[CT]
3644 GCCGGC
DraII 1399 AGGACCT [GA]G^G[ACGT]CC[CT]
3994 GGGGCCT
DraIII 423 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
1729 CACTTTGTG
Eco47III 1113 AGCGCT AGC^GCT
1748 AGCGCT
Eco81I 2970 CCTGAGG CC^T[ACGT]AGG
3508 CCTTAGG
EcoO109I 1399 AGGACCT [GA]G^G[ACGT]CC[CT]
3994 GGGGCCT
EcoT22I 17 ATGCAT ATGCA^T
483 ATGCAT
HindIII 1462 AAGCTT A^AGCTT
3747 AAGCTT
MamI 2149 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2681 GATATTCATC
NaeI 3640 GCCGGC GCC^GGC
3644 GCCGGC
NgoMIV 3640 GCCGGC G^CCGGC
3644 GCCGGC
NsiI 17 ATGCAT ATGCA^T
483 ATGCAT
SmlI 2900 CTCAAG C^T[CT][GA]AG
2940 CTCAAG
SspI 1541 AATATT AAT^ATT
2423 AATATT
StuI 2519 AGGCCT AGG^CCT
4052 AGGCCT
XmnI 1628 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3423 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII