################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AccII AclI AflII AgeI AhdI ApaI AscI Asp718I AspEI AspI AviII AvrII BcgI BciVI BcnI BfrI BlnI BsiWI BsmBI BspDI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I ClaI CpoI DrdI Eam1105I EcoO65I EcoRI EcoRV FauI FspI HpaI KpnI KspI MfeI MluI MunI MvnI NciI NcoI NdeI NotI NruI PacI PaeR7I PflFI PinAI PshAI Psp1406I RsrII SacII SalI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 52 TCCGGA T^CCGGA AcyI 2920 GGCGCC G[GA]^CG[CT]C AseI 2420 ATTAAT AT^TAAT AsnI 2420 ATTAAT AT^TAAT BalI 1764 TGGCCA TGG^CCA BamHI 511 GGATCC G^GATCC BbeI 2920 GGCGCC GGCGC^C BbrPI 634 CACGTG CAC^GTG BclI 925 TGATCA T^GATCA BsaAI 634 CACGTG [CT]AC^GT[GA] BsaHI 2920 GGCGCC G[GA]^CG[CT]C BseAI 52 TCCGGA T^CCGGA BsgI 575 GTGCAG GTGCAG(16/14) BspEI 52 TCCGGA T^CCGGA BspHI 811 TCATGA T^CATGA BstBI 1327 TTCGAA TT^CGAA DsaI 183 CCACGG C^C[GA][CT]GG EagI 3642 CGGCCG C^GGCCG EclXI 3642 CGGCCG C^GGCCG Eco52I 3642 CGGCCG C^GGCCG EcoNI 3267 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 925 TGATCA T^GATCA FseI 3639 GGCCGGCC GGCCGG^CC Hin1I 2920 GGCGCC G[GA]^CG[CT]C HincII 3392 GTTGAC GT[CT]^[GA]AC HindII 3392 GTTGAC GT[CT]^[GA]AC KasI 2920 GGCGCC G^GCGCC MaeII 635 ACGT A^CGT MluNI 1764 TGGCCA TGG^CCA MroI 52 TCCGGA T^CCGGA MscI 1764 TGGCCA TGG^CCA NarI 2920 GGCGCC GG^CGCC NheI 4040 GCTAGC G^CTAGC NspV 1327 TTCGAA TT^CGAA PflMI 2430 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 634 CACGTG CAC^GTG PmeI 1051 GTTTAAAC GTTT^AAAC PmlI 634 CACGTG CAC^GTG PpuMI 1399 AGGACCT [GA]G^G[AT]CC[CT] PshBI 2420 ATTAAT AT^TAAT PvuI 827 CGATCG CGAT^CG RcaI 811 TCATGA T^CATGA SacI 281 GAGCTC GAGCT^C SbfI 1403 CCTGCAGG CCTGCA^GG SfoI 2920 GGCGCC GGC^GCC SfuI 1327 TTCGAA TT^CGAA SphI 1143 GCATGC GCATG^C Sse8387I 1403 CCTGCAGG CCTGCA^GG SwaI 1029 ATTTAAAT ATTT^AAAT Van91I 2430 CCAGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccI 1691 GTAGAC GT^[AC][GT]AC 4005 GTAGAC Alw44I 3131 GTGCAC G^TGCAC 3241 GTGCAC AlwNI 993 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 2431 CAGCAACTG AocI 2970 CCTGAGG CC^T[ACGT]AGG 3508 CCTTAGG Aor51HI 1113 AGCGCT AGC^GCT 1748 AGCGCT ApaLI 3131 GTGCAC G^TGCAC 3241 GTGCAC Asp700I 1628 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3423 GAAGGAGTTC BanII 281 GAGCTC G[GA]GC[CT]^C 1567 GGGCTC BbvCI 2332 CCTCAGC CCTCAGC(-5/-2) 1166 GCTGAGG BglI 3014 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3095 GCCCAGTGGGC BglII 2904 AGATCT A^GATCT 3839 AGATCT BlpI 3560 GCTCAGC GC^T[ACGT]AGC 3749 GCTTAGC Bpu1102I 3560 GCTCAGC GC^T[ACGT]AGC 3749 GCTTAGC BsaBI 2149 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2681 GATATTCATC BsmI 3895 GAATGC GAATGC(1/-1) 301 GCATTC BsrDI 4027 CATTGC GCAATG(2/0) 1737 GCAATG BsrFI 3640 GCCGGC [GA]^CCGG[CT] 3644 GCCGGC BstXI 1345 CCAGAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1575 CCACCTCTGTGG Bsu36I 2970 CCTGAGG CC^T[ACGT]AGG 3508 CCTTAGG CelII 3560 GCTCAGC GC^T[ACGT]AGC 3749 GCTTAGC Cfr10I 3640 GCCGGC [GA]^CCGG[CT] 3644 GCCGGC DraII 1399 AGGACCT [GA]G^G[ACGT]CC[CT] 3994 GGGGCCT DraIII 423 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 1729 CACTTTGTG Eco47III 1113 AGCGCT AGC^GCT 1748 AGCGCT Eco81I 2970 CCTGAGG CC^T[ACGT]AGG 3508 CCTTAGG EcoO109I 1399 AGGACCT [GA]G^G[ACGT]CC[CT] 3994 GGGGCCT EcoT22I 17 ATGCAT ATGCA^T 483 ATGCAT HindIII 1462 AAGCTT A^AGCTT 3747 AAGCTT MamI 2149 GATCACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2681 GATATTCATC NaeI 3640 GCCGGC GCC^GGC 3644 GCCGGC NgoMIV 3640 GCCGGC G^CCGGC 3644 GCCGGC NsiI 17 ATGCAT ATGCA^T 483 ATGCAT SmlI 2900 CTCAAG C^T[CT][GA]AG 2940 CTCAAG SspI 1541 AATATT AAT^ATT 2423 AATATT StuI 2519 AGGCCT AGG^CCT 4052 AGGCCT XmnI 1628 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3423 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaI AvaII BanI BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII