################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII BbeI BbrPI BcgI BciVI BsaAI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BspHI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1421 GACGTC GACGT^C AcyI 1421 GACGTC G[GA]^CG[CT]C AflII 837 CTTAAG C^TTAAG AflIII 3003 ACATGT A^C[GA][CT]GT AhdI 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1957 CCTGAGG CC^T[ACGT]AGG AspEI 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 575 CCTAGG C^CTAGG BbsI 762 GAAGAC GAAGAC(2/6) BclI 1213 TGATCA T^GATCA BfrI 837 CTTAAG C^TTAAG BglII 548 AGATCT A^GATCT BlnI 575 CCTAGG C^CTAGG BlpI 2857 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2857 GCTAAGC GC^T[ACGT]AGC BpuAI 762 GAAGAC GAAGAC(2/6) BsaHI 1421 GACGTC G[GA]^CG[CT]C BsmBI 2878 GAGACG CGTCTC(1/5) BsmI 537 GAATGC GAATGC(1/-1) BspDI 225 ATCGAT AT^CGAT BspLU11I 3003 ACATGT A^CATGT BsrDI 287 CATTGC GCAATG(2/0) BstAPI 827 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1957 CCTGAGG CC^T[ACGT]AGG CelII 2857 GCTAAGC GC^T[ACGT]AGC ClaI 225 ATCGAT AT^CGAT DraI 2399 TTTAAA TTT^AAA Eam1105I 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1957 CCTGAGG CC^T[ACGT]AGG FbaI 1213 TGATCA T^GATCA HgaI 2880 GACGC GACGC(5/10) Hin1I 1421 GACGTC G[GA]^CG[CT]C KspI 2920 CCGCGG CCGC^GG NaeI 2330 GCCGGC GCC^GGC NcoI 2819 CCATGG C^CATGG NgoMIV 2330 GCCGGC G^CCGGC PaeR7I 735 CTCGAG C^TCGAG PleI 31 GACTC GAGTC(4/5) SacII 2920 CCGCGG CCGC^GG ScaI 1246 AGTACT AGT^ACT SexAI 1351 ACCTGGT A^CC[AT]GGT SpeI 1436 ACTAGT A^CTAGT SphI 1690 GCATGC GCATG^C XhoI 735 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Alw44I 812 GTGCAC G^TGCAC 2999 GTGCAC ApaI 82 GGGCCC GGGCC^C 273 GGGCCC ApaLI 812 GTGCAC G^TGCAC 2999 GTGCAC BbvCI 2312 CCTCAGC CCTCAGC(-5/-2) 1065 GCTGAGG BsaBI 158 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1208 GATTCTGATC BsgI 610 CTGCAC GTGCAG(16/14) 1440 GTGCAG BsrFI 1864 ACCGGC [GA]^CCGG[CT] 2330 GCCGGC BstEII 1358 GGTCACC G^GT[ACGT]ACC 2765 GGTCACC BstPI 1358 GGTCACC G^GT[ACGT]ACC 2765 GGTCACC BstXI 2070 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2351 CCAAGGCTGTGG Cfr10I 1864 ACCGGC [GA]^CCGG[CT] 2330 GCCGGC DsaI 2819 CCATGG C^C[GA][CT]GG 2920 CCGCGG EcoO65I 1358 GGTCACC G^GT[ACGT]ACC 2765 GGTCACC HaeII 888 GGCGCT [GA]GCGC^[CT] 2301 GGCGCT MamI 158 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1208 GATTCTGATC NspI 1690 GCATGC [GA]CATG^[CT] 3003 ACATGT StuI 440 AGGCCT AGG^CCT 1696 AGGCCT TaqI 226 TCGA T^CGA 736 TCGA TthHB8I 226 TCGA T^CGA 736 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BamHI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI