##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
BbeI BbrPI BcgI BciVI BsaAI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspEI BspHI BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DraIII DrdI
EagI EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfoI SfuI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1421 GACGTC GACGT^C
AcyI 1421 GACGTC G[GA]^CG[CT]C
AflII 837 CTTAAG C^TTAAG
AflIII 3003 ACATGT A^C[GA][CT]GT
AhdI 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1957 CCTGAGG CC^T[ACGT]AGG
AspEI 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 575 CCTAGG C^CTAGG
BbsI 762 GAAGAC GAAGAC(2/6)
BclI 1213 TGATCA T^GATCA
BfrI 837 CTTAAG C^TTAAG
BglII 548 AGATCT A^GATCT
BlnI 575 CCTAGG C^CTAGG
BlpI 2857 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2857 GCTAAGC GC^T[ACGT]AGC
BpuAI 762 GAAGAC GAAGAC(2/6)
BsaHI 1421 GACGTC G[GA]^CG[CT]C
BsmBI 2878 GAGACG CGTCTC(1/5)
BsmI 537 GAATGC GAATGC(1/-1)
BspDI 225 ATCGAT AT^CGAT
BspLU11I 3003 ACATGT A^CATGT
BsrDI 287 CATTGC GCAATG(2/0)
BstAPI 827 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1957 CCTGAGG CC^T[ACGT]AGG
CelII 2857 GCTAAGC GC^T[ACGT]AGC
ClaI 225 ATCGAT AT^CGAT
DraI 2399 TTTAAA TTT^AAA
Eam1105I 2492 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1957 CCTGAGG CC^T[ACGT]AGG
FbaI 1213 TGATCA T^GATCA
HgaI 2880 GACGC GACGC(5/10)
Hin1I 1421 GACGTC G[GA]^CG[CT]C
KspI 2920 CCGCGG CCGC^GG
NaeI 2330 GCCGGC GCC^GGC
NcoI 2819 CCATGG C^CATGG
NgoMIV 2330 GCCGGC G^CCGGC
PaeR7I 735 CTCGAG C^TCGAG
PleI 31 GACTC GAGTC(4/5)
SacII 2920 CCGCGG CCGC^GG
ScaI 1246 AGTACT AGT^ACT
SexAI 1351 ACCTGGT A^CC[AT]GGT
SpeI 1436 ACTAGT A^CTAGT
SphI 1690 GCATGC GCATG^C
XhoI 735 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 812 GTGCAC G^TGCAC
2999 GTGCAC
ApaI 82 GGGCCC GGGCC^C
273 GGGCCC
ApaLI 812 GTGCAC G^TGCAC
2999 GTGCAC
BbvCI 2312 CCTCAGC CCTCAGC(-5/-2)
1065 GCTGAGG
BsaBI 158 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1208 GATTCTGATC
BsgI 610 CTGCAC GTGCAG(16/14)
1440 GTGCAG
BsrFI 1864 ACCGGC [GA]^CCGG[CT]
2330 GCCGGC
BstEII 1358 GGTCACC G^GT[ACGT]ACC
2765 GGTCACC
BstPI 1358 GGTCACC G^GT[ACGT]ACC
2765 GGTCACC
BstXI 2070 CCAGCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2351 CCAAGGCTGTGG
Cfr10I 1864 ACCGGC [GA]^CCGG[CT]
2330 GCCGGC
DsaI 2819 CCATGG C^C[GA][CT]GG
2920 CCGCGG
EcoO65I 1358 GGTCACC G^GT[ACGT]ACC
2765 GGTCACC
HaeII 888 GGCGCT [GA]GCGC^[CT]
2301 GGCGCT
MamI 158 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1208 GATTCTGATC
NspI 1690 GCATGC [GA]CATG^[CT]
3003 ACATGT
StuI 440 AGGCCT AGG^CCT
1696 AGGCCT
TaqI 226 TCGA T^CGA
736 TCGA
TthHB8I 226 TCGA T^CGA
736 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI