##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AgeI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
BbeI        BbrPI       BcgI        BciVI       BsaAI       
BsaWI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     CpoI        DraIII      DrdI        
EagI        EarI        EclXI       Eco47III    Eco52I      
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     MfeI        MluI        MroI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SapI        SbfI        SfoI        SfuI        
SgrAI       SnaBI       Sse8387I    SspBI       SspI        
SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        1421  GACGTC               GACGT^C
AcyI         1421  GACGTC               G[GA]^CG[CT]C
AflII         837  CTTAAG               C^TTAAG
AflIII       3003  ACATGT               A^C[GA][CT]GT
AhdI         2492  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1957  CCTGAGG              CC^T[ACGT]AGG
AspEI        2492  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII         575  CCTAGG               C^CTAGG
BbsI          762  GAAGAC               GAAGAC(2/6)
BclI         1213  TGATCA               T^GATCA
BfrI          837  CTTAAG               C^TTAAG
BglII         548  AGATCT               A^GATCT
BlnI          575  CCTAGG               C^CTAGG
BlpI         2857  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2857  GCTAAGC              GC^T[ACGT]AGC
BpuAI         762  GAAGAC               GAAGAC(2/6)
BsaHI        1421  GACGTC               G[GA]^CG[CT]C
BsmBI        2878  GAGACG               CGTCTC(1/5)
BsmI          537  GAATGC               GAATGC(1/-1)
BspDI         225  ATCGAT               AT^CGAT
BspLU11I     3003  ACATGT               A^CATGT
BsrDI         287  CATTGC               GCAATG(2/0)
BstAPI        827  GCAGTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1957  CCTGAGG              CC^T[ACGT]AGG
CelII        2857  GCTAAGC              GC^T[ACGT]AGC
ClaI          225  ATCGAT               AT^CGAT
DraI         2399  TTTAAA               TTT^AAA
Eam1105I     2492  GACCCGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1957  CCTGAGG              CC^T[ACGT]AGG
FbaI         1213  TGATCA               T^GATCA
HgaI         2880  GACGC                GACGC(5/10)
Hin1I        1421  GACGTC               G[GA]^CG[CT]C
KspI         2920  CCGCGG               CCGC^GG
NaeI         2330  GCCGGC               GCC^GGC
NcoI         2819  CCATGG               C^CATGG
NgoMIV       2330  GCCGGC               G^CCGGC
PaeR7I        735  CTCGAG               C^TCGAG
PleI           31  GACTC                GAGTC(4/5)
SacII        2920  CCGCGG               CCGC^GG
ScaI         1246  AGTACT               AGT^ACT
SexAI        1351  ACCTGGT              A^CC[AT]GGT
SpeI         1436  ACTAGT               A^CTAGT
SphI         1690  GCATGC               GCATG^C
XhoI          735  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Alw44I        812  GTGCAC               G^TGCAC
             2999  GTGCAC              
ApaI           82  GGGCCC               GGGCC^C
              273  GGGCCC              
ApaLI         812  GTGCAC               G^TGCAC
             2999  GTGCAC              
BbvCI        2312  CCTCAGC              CCTCAGC(-5/-2)
             1065  GCTGAGG             
BsaBI         158  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1208  GATTCTGATC          
BsgI          610  CTGCAC               GTGCAG(16/14)
             1440  GTGCAG              
BsrFI        1864  ACCGGC               [GA]^CCGG[CT]
             2330  GCCGGC              
BstEII       1358  GGTCACC              G^GT[ACGT]ACC
             2765  GGTCACC             
BstPI        1358  GGTCACC              G^GT[ACGT]ACC
             2765  GGTCACC             
BstXI        2070  CCAGCTTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2351  CCAAGGCTGTGG        
Cfr10I       1864  ACCGGC               [GA]^CCGG[CT]
             2330  GCCGGC              
DsaI         2819  CCATGG               C^C[GA][CT]GG
             2920  CCGCGG              
EcoO65I      1358  GGTCACC              G^GT[ACGT]ACC
             2765  GGTCACC             
HaeII         888  GGCGCT               [GA]GCGC^[CT]
             2301  GGCGCT              
MamI          158  GATCCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1208  GATTCTGATC          
NspI         1690  GCATGC               [GA]CATG^[CT]
             3003  ACATGT              
StuI          440  AGGCCT               AGG^CCT
             1696  AGGCCT              
TaqI          226  TCGA                 T^CGA
              736  TCGA                
TthHB8I       226  TCGA                 T^CGA
              736  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BamHI       BanI        BanII       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BsaI        
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PflMI       PpuMI       PstI        PvuII       RsaI        
SacI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfiI        SmaI        SmlI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        XhoII       XmaCI       
XmaI