##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BcgI BciVI
BclI BglI BglII BlnI BlpI
Bpu1102I BsiWI BspDI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraIII EaeI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI KspI MfeI MluI MluNI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 65 GACGTC GACGT^C
AccII 1639 CGCG CG^CG
AflII 2422 CTTAAG C^TTAAG
AflIII 763 ACACGT A^C[GA][CT]GT
AhdI 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 407 CACGTG CAC^GTG
BbvCI 203 GCTGAGG CCTCAGC(-5/-2)
BfrI 2422 CTTAAG C^TTAAG
BsaBI 1912 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 211 CGATCG CG[GA][CT]^CG
BsmI 733 GAATGC GAATGC(1/-1)
BssSI 1144 CACGAG CACGAG(-5/-1)
BstUI 1639 CGCG CG^CG
Eam1105I 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1807 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 164 GAATTC G^AATTC
HincII 440 GTTGAC GT[CT]^[GA]AC
HindII 440 GTTGAC GT[CT]^[GA]AC
HindIII 1822 AAGCTT A^AGCTT
MamI 1912 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 865 CAGCGG C[AC]G^C[GT]G
MvnI 1639 CGCG CG^CG
NaeI 104 GCCGGC GCC^GGC
NgoMIV 104 GCCGGC G^CCGGC
NspI 1456 GCATGC [GA]CATG^[CT]
PmaCI 407 CACGTG CAC^GTG
PmlI 407 CACGTG CAC^GTG
PstI 842 CTGCAG CTGCA^G
PvuI 211 CGATCG CGAT^CG
SexAI 1974 ACCTGGT A^CC[AT]GGT
SfcI 842 CTGCAG C^T[GA][CT]AG
SmaI 594 CCCGGG CCC^GGG
SphI 1456 GCATGC GCATG^C
TfiI 2147 GATTC G^A[AT]TC
XcmI 2589 CCAGTAAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 594 CCCGGG C^CCGGG
XmaI 594 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 957 GTAGAC GT^[AC][GT]AC
2526 GTCTAC
AccIII 286 TCCGGA T^CCGGA
346 TCCGGA
AcyI 65 GACGTC G[GA]^CG[CT]C
869 GGCGTC
Alw44I 404 GTGCAC G^TGCAC
1160 GTGCAC
ApaLI 404 GTGCAC G^TGCAC
1160 GTGCAC
Asp700I 1105 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2142 GAAGAGATTC
BbsI 2348 GAAGAC GAAGAC(2/6)
931 GTCTTC
BpuAI 2348 GAAGAC GAAGAC(2/6)
931 GTCTTC
BsaAI 407 CACGTG [CT]AC^GT[GA]
858 TACGTG
BsaHI 65 GACGTC G[GA]^CG[CT]C
869 GGCGTC
BsaWI 286 TCCGGA [AT]^CCGG[AT]
346 TCCGGA
BseAI 286 TCCGGA T^CCGGA
346 TCCGGA
BsgI 413 CTGCAC GTGCAG(16/14)
986 GTGCAG
BsmBI 67 CGTCTC CGTCTC(1/5)
1340 GAGACG
BspEI 286 TCCGGA T^CCGGA
346 TCCGGA
BspMI 1007 ACCTGC ACCTGC(4/8)
2505 ACCTGC
BsrBI 1132 CCGCTC CCGCTC(-3/-3)
477 GAGCGG
BsrFI 104 GCCGGC [GA]^CCGG[CT]
746 GCCGGT
Cfr10I 104 GCCGGC [GA]^CCGG[CT]
746 GCCGGT
DrdI 787 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2351 GACGAGAGTGTC
Hin1I 65 GACGTC G[GA]^CG[CT]C
869 GGCGTC
MroI 286 TCCGGA T^CCGGA
346 TCCGGA
PpuMI 1082 AGGACCT [GA]G^G[AT]CC[CT]
1782 AGGACCC
PshAI 376 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1208 GACCACAGTC
SmlI 1926 CTCAAG C^T[CT][GA]AG
2422 CTTAAG
XmnI 1105 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2142 GAAGAGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BamHI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII