################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AclI AgeI Aor51HI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BbeI BcgI BciVI BclI BglI BglII BlnI BlpI Bpu1102I BsiWI BspDI BspHI BspLU11I BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraIII EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 65 GACGTC GACGT^C AccII 1639 CGCG CG^CG AflII 2422 CTTAAG C^TTAAG AflIII 763 ACACGT A^C[GA][CT]GT AhdI 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 407 CACGTG CAC^GTG BbvCI 203 GCTGAGG CCTCAGC(-5/-2) BfrI 2422 CTTAAG C^TTAAG BsaBI 1912 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 211 CGATCG CG[GA][CT]^CG BsmI 733 GAATGC GAATGC(1/-1) BssSI 1144 CACGAG CACGAG(-5/-1) BstUI 1639 CGCG CG^CG Eam1105I 2455 GACCGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1807 CCTGTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 164 GAATTC G^AATTC HincII 440 GTTGAC GT[CT]^[GA]AC HindII 440 GTTGAC GT[CT]^[GA]AC HindIII 1822 AAGCTT A^AGCTT MamI 1912 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 865 CAGCGG C[AC]G^C[GT]G MvnI 1639 CGCG CG^CG NaeI 104 GCCGGC GCC^GGC NgoMIV 104 GCCGGC G^CCGGC NspI 1456 GCATGC [GA]CATG^[CT] PmaCI 407 CACGTG CAC^GTG PmlI 407 CACGTG CAC^GTG PstI 842 CTGCAG CTGCA^G PvuI 211 CGATCG CGAT^CG SexAI 1974 ACCTGGT A^CC[AT]GGT SfcI 842 CTGCAG C^T[GA][CT]AG SmaI 594 CCCGGG CCC^GGG SphI 1456 GCATGC GCATG^C TfiI 2147 GATTC G^A[AT]TC XcmI 2589 CCAGTAAGACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 594 CCCGGG C^CCGGG XmaI 594 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 957 GTAGAC GT^[AC][GT]AC 2526 GTCTAC AccIII 286 TCCGGA T^CCGGA 346 TCCGGA AcyI 65 GACGTC G[GA]^CG[CT]C 869 GGCGTC Alw44I 404 GTGCAC G^TGCAC 1160 GTGCAC ApaLI 404 GTGCAC G^TGCAC 1160 GTGCAC Asp700I 1105 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2142 GAAGAGATTC BbsI 2348 GAAGAC GAAGAC(2/6) 931 GTCTTC BpuAI 2348 GAAGAC GAAGAC(2/6) 931 GTCTTC BsaAI 407 CACGTG [CT]AC^GT[GA] 858 TACGTG BsaHI 65 GACGTC G[GA]^CG[CT]C 869 GGCGTC BsaWI 286 TCCGGA [AT]^CCGG[AT] 346 TCCGGA BseAI 286 TCCGGA T^CCGGA 346 TCCGGA BsgI 413 CTGCAC GTGCAG(16/14) 986 GTGCAG BsmBI 67 CGTCTC CGTCTC(1/5) 1340 GAGACG BspEI 286 TCCGGA T^CCGGA 346 TCCGGA BspMI 1007 ACCTGC ACCTGC(4/8) 2505 ACCTGC BsrBI 1132 CCGCTC CCGCTC(-3/-3) 477 GAGCGG BsrFI 104 GCCGGC [GA]^CCGG[CT] 746 GCCGGT Cfr10I 104 GCCGGC [GA]^CCGG[CT] 746 GCCGGT DrdI 787 GACCAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2351 GACGAGAGTGTC Hin1I 65 GACGTC G[GA]^CG[CT]C 869 GGCGTC MroI 286 TCCGGA T^CCGGA 346 TCCGGA PpuMI 1082 AGGACCT [GA]G^G[AT]CC[CT] 1782 AGGACCC PshAI 376 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1208 GACCACAGTC SmlI 1926 CTCAAG C^T[CT][GA]AG 2422 CTTAAG XmnI 1105 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2142 GAAGAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BamHI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII