##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbrPI BbsI BclI BfrI
BglII BlnI BpuAI BsaBI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BssHII BssSI BstAPI BstEII BstPI
ClaI CpoI Eam1105I Eco47III EcoO65I
EcoRI EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NdeI NotI
NruI NsiI PacI PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SalI SbfI ScaI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1636 GTATAC GT^[AC][GT]AC
AccIII 2492 TCCGGA T^CCGGA
AflIII 1900 ACACGT A^C[GA][CT]GT
AgeI 1229 ACCGGT A^CCGGT
Asp700I 1597 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 2380 GGATCC G^GATCC
BbeI 1069 GGCGCC GGCGC^C
BbvCI 688 CCTCAGC CCTCAGC(-5/-2)
BciVI 1297 GTATCC GTATCC(6/5)
BlpI 2410 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2410 GCTCAGC GC^T[ACGT]AGC
BsaAI 2685 CACGTA [CT]AC^GT[GA]
BseAI 2492 TCCGGA T^CCGGA
BspEI 2492 TCCGGA T^CCGGA
BspMI 1854 GCAGGT ACCTGC(4/8)
BsrBI 28 GAGCGG CCGCTC(-3/-3)
BsrDI 731 CATTGC GCAATG(2/0)
Bst1107I 1636 GTATAC GTA^TAC
BstBI 1040 TTCGAA TT^CGAA
BstZ17I 1636 GTATAC GTA^TAC
CelII 2410 GCTCAGC GC^T[ACGT]AGC
DraI 2924 TTTAAA TTT^AAA
DraIII 2603 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2253 CGGCCG C^GGCCG
EclXI 2253 CGGCCG C^GGCCG
Eco52I 2253 CGGCCG C^GGCCG
EcoRV 1498 GATATC GAT^ATC
HgaI 363 GACGC GACGC(5/10)
KasI 1069 GGCGCC G^GCGCC
MroI 2492 TCCGGA T^CCGGA
NaeI 413 GCCGGC GCC^GGC
NarI 1069 GGCGCC GG^CGCC
NgoMIV 413 GCCGGC G^CCGGC
NheI 2137 GCTAGC G^CTAGC
NspV 1040 TTCGAA TT^CGAA
PaeR7I 2835 CTCGAG C^TCGAG
PinAI 1229 ACCGGT A^CCGGT
PvuI 39 CGATCG CGAT^CG
SexAI 2604 ACCAGGT A^CC[AT]GGT
SfoI 1069 GGCGCC GGC^GCC
SfuI 1040 TTCGAA TT^CGAA
SspI 482 AATATT AAT^ATT
XcmI 2309 CCAAGGAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2835 CTCGAG C^TCGAG
XmnI 1597 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 363 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
AocI 559 CCTGAGG CC^T[ACGT]AGG
1871 CCTCAGG
BcgI 7 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
33 GCAGACCGATCG
BglI 45 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
209 GCCTGACGGGC
BpmI 784 CTCCAG CTGGAG(16/14)
2477 CTGGAG
BsaHI 363 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
BsaI 373 GAGACC GGTCTC(1/5)
388 GAGACC
BseRI 545 CTCCTC GAGGAG(10/8)
1396 GAGGAG
BsiEI 39 CGATCG CG[GA][CT]^CG
2253 CGGCCG
Bsp1407I 1948 TGTACA T^GTACA
2579 TGTACA
BsrGI 1948 TGTACA T^GTACA
2579 TGTACA
BstXI 221 CCACCGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2801 CCATTGATTTGG
Bsu36I 559 CCTGAGG CC^T[ACGT]AGG
1871 CCTCAGG
DrdI 1270 GACTTCAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2095 GACCCAAGTGTC
Eco81I 559 CCTGAGG CC^T[ACGT]AGG
1871 CCTCAGG
Hin1I 363 GACGCC G[GA]^CG[CT]C
1069 GGCGCC
MslI 222 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1842 CACAGCAGTG
NspI 1344 GCATGT [GA]CATG^[CT]
2672 GCATGT
PleI 2465 GACTC GAGTC(4/5)
2750 GACTC
PpuMI 1535 GGGACCT [GA]G^G[AT]CC[CT]
2093 GGGACCC
PstI 2027 CTGCAG CTGCA^G
2375 CTGCAG
SacI 1450 GAGCTC GAGCT^C
2149 GAGCTC
SfaNI 1936 GATGC GCATC(5/9)
875 GCATC
SfiI 44 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
208 GGCCTGACGGGCC
SmaI 292 CCCGGG CCC^GGG
2351 CCCGGG
SmlI 548 CTCAAG C^T[CT][GA]AG
2835 CTCGAG
SspBI 1948 TGTACA T^GTACA
2579 TGTACA
TfiI 723 GAATC G^A[AT]TC
1098 GAATC
XmaCI 292 CCCGGG C^CCGGG
2351 CCCGGG
XmaI 292 CCCGGG C^CCGGG
2351 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BsaJI
BsaWI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PvuII RsaI SacII SapI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII