##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp700I AspEI AviII BbeI
BbrPI BciVI BfrI BglII BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrFI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
StuI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflIII 4661 ACATGT A^C[GA][CT]GT
Alw44I 388 GTGCAC G^TGCAC
AocI 4166 CCTCAGG CC^T[ACGT]AGG
ApaLI 388 GTGCAC G^TGCAC
AspI 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1749 CCTAGG C^CTAGG
BcgI 1510 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4667 TGATCA T^GATCA
BlnI 1749 CCTAGG C^CTAGG
BsaBI 630 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2855 GCATTC GAATGC(1/-1)
BspLU11I 4661 ACATGT A^CATGT
BssSI 1235 CACGAG CACGAG(-5/-1)
BstEII 1314 GGTCACC G^GT[ACGT]ACC
BstPI 1314 GGTCACC G^GT[ACGT]ACC
Bsu36I 4166 CCTCAGG CC^T[ACGT]AGG
DraIII 2222 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 981 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 4166 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1314 GGTCACC G^GT[ACGT]ACC
EcoRI 3483 GAATTC G^AATTC
FbaI 4667 TGATCA T^GATCA
HaeII 2761 AGCGCC [GA]GCGC^[CT]
HgaI 192 GACGC GACGC(5/10)
MaeII 3546 ACGT A^CGT
MamI 630 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2396 CATATG CA^TATG
PflFI 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 1675 CCTGCAGG CCTGCA^GG
ScaI 1693 AGTACT AGT^ACT
SmaI 1445 CCCGGG CCC^GGG
SphI 2368 GCATGC GCATG^C
Sse8387I 1675 CCTGCAGG CCTGCA^GG
SspI 4678 AATATT AAT^ATT
Tth111I 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1445 CCCGGG C^CCGGG
XmaI 1445 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
Acc65I 532 GGTACC G^GTACC
2235 GGTACC
AccI 412 GTAGAC GT^[AC][GT]AC
2656 GTCTAC
AccII 194 CGCG CG^CG
4263 CGCG
AcsI 3097 AAATTC [GA]^AATT[CT]
3483 GAATTC
ApoI 3097 AAATTC [GA]^AATT[CT]
3483 GAATTC
Asp718I 532 GGTACC G^GTACC
2235 GGTACC
BamHI 864 GGATCC G^GATCC
1441 GGATCC
BsaI 1110 GAGACC GGTCTC(1/5)
3007 GAGACC
BstUI 194 CGCG CG^CG
4263 CGCG
EcoNI 520 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1749 CCTAGGCAAGG
KpnI 532 GGTACC GGTAC^C
2235 GGTACC
MvnI 194 CGCG CG^CG
4263 CGCG
NcoI 1310 CCATGG C^CATGG
3738 CCATGG
SapI 3364 GAAGAGC GCTCTTC(1/4)
3726 GCTCTTC
SmlI 703 CTCAAG C^T[CT][GA]AG
2965 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SpeI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII