################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BbeI BbrPI BciVI BfrI BglII BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AflIII 4661 ACATGT A^C[GA][CT]GT Alw44I 388 GTGCAC G^TGCAC AocI 4166 CCTCAGG CC^T[ACGT]AGG ApaLI 388 GTGCAC G^TGCAC AspI 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1749 CCTAGG C^CTAGG BcgI 1510 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 4667 TGATCA T^GATCA BlnI 1749 CCTAGG C^CTAGG BsaBI 630 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 2855 GCATTC GAATGC(1/-1) BspLU11I 4661 ACATGT A^CATGT BssSI 1235 CACGAG CACGAG(-5/-1) BstEII 1314 GGTCACC G^GT[ACGT]ACC BstPI 1314 GGTCACC G^GT[ACGT]ACC Bsu36I 4166 CCTCAGG CC^T[ACGT]AGG DraIII 2222 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 981 GACCCAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4166 CCTCAGG CC^T[ACGT]AGG EcoO65I 1314 GGTCACC G^GT[ACGT]ACC EcoRI 3483 GAATTC G^AATTC FbaI 4667 TGATCA T^GATCA HaeII 2761 AGCGCC [GA]GCGC^[CT] HgaI 192 GACGC GACGC(5/10) MaeII 3546 ACGT A^CGT MamI 630 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 2396 CATATG CA^TATG PflFI 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC SbfI 1675 CCTGCAGG CCTGCA^GG ScaI 1693 AGTACT AGT^ACT SmaI 1445 CCCGGG CCC^GGG SphI 2368 GCATGC GCATG^C Sse8387I 1675 CCTGCAGG CCTGCA^GG SspI 4678 AATATT AAT^ATT Tth111I 3337 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1445 CCCGGG C^CCGGG XmaI 1445 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### Acc65I 532 GGTACC G^GTACC 2235 GGTACC AccI 412 GTAGAC GT^[AC][GT]AC 2656 GTCTAC AccII 194 CGCG CG^CG 4263 CGCG AcsI 3097 AAATTC [GA]^AATT[CT] 3483 GAATTC ApoI 3097 AAATTC [GA]^AATT[CT] 3483 GAATTC Asp718I 532 GGTACC G^GTACC 2235 GGTACC BamHI 864 GGATCC G^GATCC 1441 GGATCC BsaI 1110 GAGACC GGTCTC(1/5) 3007 GAGACC BstUI 194 CGCG CG^CG 4263 CGCG EcoNI 520 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1749 CCTAGGCAAGG KpnI 532 GGTACC GGTAC^C 2235 GGTACC MvnI 194 CGCG CG^CG 4263 CGCG NcoI 1310 CCATGG C^CATGG 3738 CCATGG SapI 3364 GAAGAGC GCTCTTC(1/4) 3726 GCTCTTC SmlI 703 CTCAAG C^T[CT][GA]AG 2965 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SpeI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII