##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AccI AflII AhdI AscI AseI
AsnI AspEI AspI BcgI BclI
BfrI BglII BsaBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
MamI MfeI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmeI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SfiI SfuI SnaBI Sse8387I
SspBI SspI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 3454 GACGTC GACGT^C
Acc65I 2812 GGTACC G^GTACC
AccIII 101 TCCGGA T^CCGGA
AclI 2684 AACGTT AA^CGTT
AcsI 3432 GAATTC [GA]^AATT[CT]
AflIII 2729 ACGCGT A^C[GA][CT]GT
AgeI 1372 ACCGGT A^CCGGT
Alw44I 37 GTGCAC G^TGCAC
Aor51HI 904 AGCGCT AGC^GCT
ApaLI 37 GTGCAC G^TGCAC
ApoI 3432 GAATTC [GA]^AATT[CT]
Asp700I 2030 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2812 GGTACC G^GTACC
AvrII 3514 CCTAGG C^CTAGG
BamHI 44 GGATCC G^GATCC
BbeI 1173 GGCGCC GGCGC^C
BbrPI 2952 CACGTG CAC^GTG
BciVI 3263 GTATCC GTATCC(6/5)
BlnI 3514 CCTAGG C^CTAGG
BsaAI 2952 CACGTG [CT]AC^GT[GA]
BseAI 101 TCCGGA T^CCGGA
BsiEI 1844 CGGTCG CG[GA][CT]^CG
BsiWI 2707 CGTACG C^GTACG
BspEI 101 TCCGGA T^CCGGA
BstYI 44 GGATCC [GA]^GATC[CT]
Eco47III 904 AGCGCT AGC^GCT
EcoNI 2786 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3432 GAATTC G^AATTC
KasI 1173 GGCGCC G^GCGCC
KpnI 2812 GGTACC GGTAC^C
KspI 2713 CCGCGG CCGC^GG
MflI 44 GGATCC [GA]^GATC[CT]
MluI 2729 ACGCGT A^CGCGT
MroI 101 TCCGGA T^CCGGA
NarI 1173 GGCGCC GG^CGCC
NspI 1688 GCATGC [GA]CATG^[CT]
PinAI 1372 ACCGGT A^CCGGT
PmaCI 2952 CACGTG CAC^GTG
PmlI 2952 CACGTG CAC^GTG
PshAI 1446 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2684 AACGTT AA^CGTT
SacII 2713 CCGCGG CCGC^GG
SexAI 1513 ACCAGGT A^CC[AT]GGT
SfoI 1173 GGCGCC GGC^GCC
SgrAI 1371 CACCGGTG C[GA]^CCGG[CT]G
SpeI 1820 ACTAGT A^CTAGT
SphI 1688 GCATGC GCATG^C
XcmI 1714 CCACCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 44 GGATCC [GA]^GATC[CT]
XmnI 2030 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwI 45 GATCC GGATC(4/5)
44 GGATC
ApaI 2007 GGGCCC GGGCC^C
2116 GGGCCC
AviII 1210 TGCGCA TGC^GCA
1393 TGCGCA
BbsI 1491 GTCTTC GAAGAC(2/6)
2034 GTCTTC
BpuAI 1491 GTCTTC GAAGAC(2/6)
2034 GTCTTC
BstEII 1452 GGTCACC G^GT[ACGT]ACC
2336 GGTCACC
BstPI 1452 GGTCACC G^GT[ACGT]ACC
2336 GGTCACC
EcoO65I 1452 GGTCACC G^GT[ACGT]ACC
2336 GGTCACC
FspI 1210 TGCGCA TGC^GCA
1393 TGCGCA
HaeII 904 AGCGCT [GA]GCGC^[CT]
1173 GGCGCC
MseI 2410 TTAA T^TAA
3010 TTAA
NcoI 3360 CCATGG C^CATGG
4184 CCATGG
NheI 647 GCTAGC G^CTAGC
1778 GCTAGC
SacI 1049 GAGCTC GAGCT^C
1910 GAGCTC
Tru9I 2410 TTAA T^TAA
3010 TTAA
Tsp509I 3014 AATT ^AATT
3433 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XmaCI XmaI