################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AccI AflII AhdI AscI AseI AsnI AspEI AspI BcgI BclI BfrI BglII BsaBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI MamI MfeI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmeI PshBI PvuI RcaI RsrII SalI SapI SbfI ScaI SfiI SfuI SnaBI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 3454 GACGTC GACGT^C Acc65I 2812 GGTACC G^GTACC AccIII 101 TCCGGA T^CCGGA AclI 2684 AACGTT AA^CGTT AcsI 3432 GAATTC [GA]^AATT[CT] AflIII 2729 ACGCGT A^C[GA][CT]GT AgeI 1372 ACCGGT A^CCGGT Alw44I 37 GTGCAC G^TGCAC Aor51HI 904 AGCGCT AGC^GCT ApaLI 37 GTGCAC G^TGCAC ApoI 3432 GAATTC [GA]^AATT[CT] Asp700I 2030 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2812 GGTACC G^GTACC AvrII 3514 CCTAGG C^CTAGG BamHI 44 GGATCC G^GATCC BbeI 1173 GGCGCC GGCGC^C BbrPI 2952 CACGTG CAC^GTG BciVI 3263 GTATCC GTATCC(6/5) BlnI 3514 CCTAGG C^CTAGG BsaAI 2952 CACGTG [CT]AC^GT[GA] BseAI 101 TCCGGA T^CCGGA BsiEI 1844 CGGTCG CG[GA][CT]^CG BsiWI 2707 CGTACG C^GTACG BspEI 101 TCCGGA T^CCGGA BstYI 44 GGATCC [GA]^GATC[CT] Eco47III 904 AGCGCT AGC^GCT EcoNI 2786 CCTGCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 3432 GAATTC G^AATTC KasI 1173 GGCGCC G^GCGCC KpnI 2812 GGTACC GGTAC^C KspI 2713 CCGCGG CCGC^GG MflI 44 GGATCC [GA]^GATC[CT] MluI 2729 ACGCGT A^CGCGT MroI 101 TCCGGA T^CCGGA NarI 1173 GGCGCC GG^CGCC NspI 1688 GCATGC [GA]CATG^[CT] PinAI 1372 ACCGGT A^CCGGT PmaCI 2952 CACGTG CAC^GTG PmlI 2952 CACGTG CAC^GTG PshAI 1446 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2684 AACGTT AA^CGTT SacII 2713 CCGCGG CCGC^GG SexAI 1513 ACCAGGT A^CC[AT]GGT SfoI 1173 GGCGCC GGC^GCC SgrAI 1371 CACCGGTG C[GA]^CCGG[CT]G SpeI 1820 ACTAGT A^CTAGT SphI 1688 GCATGC GCATG^C XcmI 1714 CCACCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 44 GGATCC [GA]^GATC[CT] XmnI 2030 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 45 GATCC GGATC(4/5) 44 GGATC ApaI 2007 GGGCCC GGGCC^C 2116 GGGCCC AviII 1210 TGCGCA TGC^GCA 1393 TGCGCA BbsI 1491 GTCTTC GAAGAC(2/6) 2034 GTCTTC BpuAI 1491 GTCTTC GAAGAC(2/6) 2034 GTCTTC BstEII 1452 GGTCACC G^GT[ACGT]ACC 2336 GGTCACC BstPI 1452 GGTCACC G^GT[ACGT]ACC 2336 GGTCACC EcoO65I 1452 GGTCACC G^GT[ACGT]ACC 2336 GGTCACC FspI 1210 TGCGCA TGC^GCA 1393 TGCGCA HaeII 904 AGCGCT [GA]GCGC^[CT] 1173 GGCGCC MseI 2410 TTAA T^TAA 3010 TTAA NcoI 3360 CCATGG C^CATGG 4184 CCATGG NheI 647 GCTAGC G^CTAGC 1778 GCTAGC SacI 1049 GAGCTC GAGCT^C 1910 GAGCTC Tru9I 2410 TTAA T^TAA 3010 TTAA Tsp509I 3014 AATT ^AATT 3433 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XmaCI XmaI