##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AvaI AviII BamHI BcgI
BclI BglI BlpI Bpu1102I BseAI
BsiEI BsiWI BsoBI BspEI BssHII
BstBI BstEII BstPI CelII CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI KpnI KspI MfeI
MluI MroI MunI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SphI Sse8387I SwaI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 4275 GTATAC GT^[AC][GT]AC
AccII 953 CGCG CG^CG
AflII 3800 CTTAAG C^TTAAG
AgeI 4208 ACCGGT A^CCGGT
AhdI 693 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 693 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3479 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3451 TGGCCA TGG^CCA
BbsI 690 GAAGAC GAAGAC(2/6)
BciVI 246 GGATAC GTATCC(6/5)
BfrI 3800 CTTAAG C^TTAAG
BglII 1441 AGATCT A^GATCT
BpuAI 690 GAAGAC GAAGAC(2/6)
BsaWI 4208 ACCGGT [AT]^CCGG[AT]
BsmBI 4245 CGTCTC CGTCTC(1/5)
BsmI 2130 GAATGC GAATGC(1/-1)
BspDI 1027 ATCGAT AT^CGAT
BspHI 1132 TCATGA T^CATGA
BsrDI 366 CATTGC GCAATG(2/0)
BssSI 2070 CACGAG CACGAG(-5/-1)
Bst1107I 4275 GTATAC GTA^TAC
BstUI 953 CGCG CG^CG
BstZ17I 4275 GTATAC GTA^TAC
ClaI 1027 ATCGAT AT^CGAT
EaeI 3451 TGGCCA [CT]^GGCC[GA]
Eam1105I 693 GACAAGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3805 GAATTC G^AATTC
FauI 980 CCCGC CCCGC(4/6)
HpaI 3511 GTTAAC GTT^AAC
MluNI 3451 TGGCCA TGG^CCA
MscI 3451 TGGCCA TGG^CCA
MvnI 953 CGCG CG^CG
NcoI 398 CCATGG C^CATGG
PflFI 3479 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4208 ACCGGT A^CCGGT
RcaI 1132 TCATGA T^CATGA
SnaBI 127 TACGTA TAC^GTA
SpeI 2882 ACTAGT A^CTAGT
Tth111I 3479 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Alw44I 729 GTGCAC G^TGCAC
3258 GTGCAC
ApaLI 729 GTGCAC G^TGCAC
3258 GTGCAC
AvrII 2099 CCTAGG C^CTAGG
3573 CCTAGG
BbeI 1298 GGCGCC GGCGC^C
1690 GGCGCC
BbrPI 537 CACGTG CAC^GTG
3665 CACGTG
BcnI 1051 CCCGG CC^[GC]GG
1149 CCGGG
BlnI 2099 CCTAGG C^CTAGG
3573 CCTAGG
BsaBI 956 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3213 GATGTGCATC
BsaI 2390 GGTCTC GGTCTC(1/5)
3935 GGTCTC
BspMI 1319 ACCTGC ACCTGC(4/8)
3932 GCAGGT
BsrFI 3532 GCCGGT [GA]^CCGG[CT]
4208 ACCGGT
BstAPI 1166 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1889 GCAGACCCTGC
BstXI 234 CCATACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1873 CCACCACCGTGG
Cfr10I 3532 GCCGGT [GA]^CCGG[CT]
4208 ACCGGT
DraII 1759 AGGGCCT [GA]G^G[ACGT]CC[CT]
2706 AGGCCCC
DsaI 398 CCATGG C^C[GA][CT]GG
1879 CCGTGG
EcoO109I 1759 AGGGCCT [GA]G^G[ACGT]CC[CT]
2706 AGGCCCC
EcoT22I 210 ATGCAT ATGCA^T
363 ATGCAT
HaeII 1298 GGCGCC [GA]GCGC^[CT]
1690 GGCGCC
HincII 3511 GTTAAC GT[CT]^[GA]AC
3898 GTCAAC
HindII 3511 GTTAAC GT[CT]^[GA]AC
3898 GTCAAC
HindIII 873 AAGCTT A^AGCTT
2139 AAGCTT
KasI 1298 GGCGCC G^GCGCC
1690 GGCGCC
MamI 956 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3213 GATGTGCATC
NarI 1298 GGCGCC GG^CGCC
1690 GGCGCC
NciI 1051 CCCGG CC^[GC]GG
1149 CCGGG
NsiI 210 ATGCAT ATGCA^T
363 ATGCAT
PmaCI 537 CACGTG CAC^GTG
3665 CACGTG
PmlI 537 CACGTG CAC^GTG
3665 CACGTG
PstI 1173 CTGCAG CTGCA^G
3426 CTGCAG
SacI 1239 GAGCTC GAGCT^C
4230 GAGCTC
ScaI 336 AGTACT AGT^ACT
4369 AGTACT
SfoI 1298 GGCGCC GGC^GCC
1690 GGCGCC
XcmI 4138 CCACTCTGTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4338 CCAAGTAACAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbvCI
BbvI BfaI BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrBI BsrGI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII