##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
AscI AseI AsnI AvrII BamHI
BciVI BclI BglII BlnI BsaI
BsiEI BsiWI BspDI BspHI BspLU11I
BsrDI BssSI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I EcoRV FbaI FseI HindIII
HpaI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2178 GGTACC G^GTACC
AccIII 2928 TCCGGA T^CCGGA
AflII 661 CTTAAG C^TTAAG
AhdI 1989 GACCAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1569 GTGCAC G^TGCAC
ApaLI 1569 GTGCAC G^TGCAC
Asp700I 143 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2178 GGTACC G^GTACC
AspEI 1989 GACCAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2036 GGCGCC GGCGC^C
BbvCI 1031 CCTCAGC CCTCAGC(-5/-2)
BfrI 661 CTTAAG C^TTAAG
BsaBI 419 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2928 TCCGGA T^CCGGA
BsmBI 1843 CGTCTC CGTCTC(1/5)
BspEI 2928 TCCGGA T^CCGGA
BsrBI 2778 CCGCTC CCGCTC(-3/-3)
DraI 195 TTTAAA TTT^AAA
DraIII 1525 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2932 GACGCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1989 GACCAACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1198 GAATTC G^AATTC
EcoT22I 1327 ATGCAT ATGCA^T
HincII 350 GTTGAC GT[CT]^[GA]AC
HindII 350 GTTGAC GT[CT]^[GA]AC
KasI 2036 GGCGCC G^GCGCC
KpnI 2178 GGTACC GGTAC^C
MamI 419 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2928 TCCGGA T^CCGGA
NarI 2036 GGCGCC GG^CGCC
NcoI 2999 CCATGG C^CATGG
NsiI 1327 ATGCAT ATGCA^T
SapI 373 GCTCTTC GCTCTTC(1/4)
ScaI 1167 AGTACT AGT^ACT
SexAI 1972 ACCTGGT A^CC[AT]GGT
SfoI 2036 GGCGCC GGC^GCC
SphI 267 GCATGC GCATG^C
XmnI 143 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 974 AAATTC [GA]^AATT[CT]
1198 GAATTC
AflIII 2525 ACGTGT A^C[GA][CT]GT
2672 ACGTGT
AocI 1114 CCTCAGG CC^T[ACGT]AGG
2279 CCTGAGG
ApaI 2211 GGGCCC GGGCC^C
3548 GGGCCC
ApoI 974 AAATTC [GA]^AATT[CT]
1198 GAATTC
AspI 2295 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2889 GACTTCGTC
AviII 1745 TGCGCA TGC^GCA
3236 TGCGCA
BsgI 1026 CTGCAC GTGCAG(16/14)
1547 CTGCAC
BsmI 1556 GCATTC GAATGC(1/-1)
2047 GCATTC
Bsp1407I 795 TGTACA T^GTACA
2675 TGTACA
BsrGI 795 TGTACA T^GTACA
2675 TGTACA
BssHII 491 GCGCGC G^CGCGC
493 GCGCGC
BstEII 1443 GGTGACC G^GT[ACGT]ACC
3512 GGTCACC
BstPI 1443 GGTGACC G^GT[ACGT]ACC
3512 GGTCACC
BstYI 323 GGATCT [GA]^GATC[CT]
1925 GGATCT
Bsu36I 1114 CCTCAGG CC^T[ACGT]AGG
2279 CCTGAGG
EarI 374 CTCTTC CTCTTC(1/4)
1866 GAAGAG
Eco81I 1114 CCTCAGG CC^T[ACGT]AGG
2279 CCTGAGG
EcoO65I 1443 GGTGACC G^GT[ACGT]ACC
3512 GGTCACC
FspI 1745 TGCGCA TGC^GCA
3236 TGCGCA
Ksp632I 374 CTCTTC CTCTTC(1/4)
1866 GAAGAG
KspI 678 CCGCGG CCGC^GG
698 CCGCGG
MflI 323 GGATCT [GA]^GATC[CT]
1925 GGATCT
MseI 196 TTAA T^TAA
662 TTAA
NspI 267 GCATGC [GA]CATG^[CT]
3231 GCATGT
PflFI 2295 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2889 GACTTCGTC
SacII 678 CCGCGG CCGC^GG
698 CCGCGG
SmaI 745 CCCGGG CCC^GGG
2207 CCCGGG
SspBI 795 TGTACA T^GTACA
2675 TGTACA
Tru9I 196 TTAA T^TAA
662 TTAA
Tth111I 2295 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2889 GACTTCGTC
XhoII 323 GGATCT [GA]^GATC[CT]
1925 GGATCT
XmaCI 745 CCCGGG C^CCGGG
2207 CCCGGG
XmaI 745 CCCGGG C^CCGGG
2207 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI