##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI BbrPI BciVI BclI
BfrI BglII BsaAI BsaBI BsiWI
BsmBI BsmI BspDI BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 270 GACGTC GACGT^C
AccI 1602 GTCTAC GT^[AC][GT]AC
AccIII 991 TCCGGA T^CCGGA
AcsI 1563 GAATTT [GA]^AATT[CT]
AflIII 1435 ACATGT A^C[GA][CT]GT
AocI 1130 CCTGAGG CC^T[ACGT]AGG
Aor51HI 435 AGCGCT AGC^GCT
ApaI 697 GGGCCC GGGCC^C
ApoI 1563 GAATTT [GA]^AATT[CT]
Asp700I 1640 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2904 CCTAGG C^CTAGG
BamHI 75 GGATCC G^GATCC
BglI 3077 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2904 CCTAGG C^CTAGG
BsaWI 991 TCCGGA [AT]^CCGG[AT]
BseAI 991 TCCGGA T^CCGGA
Bsp1407I 1402 TGTACA T^GTACA
BspEI 991 TCCGGA T^CCGGA
BspHI 1225 TCATGA T^CATGA
BspLU11I 1435 ACATGT A^CATGT
BsrGI 1402 TGTACA T^GTACA
BstYI 75 GGATCC [GA]^GATC[CT]
Bsu36I 1130 CCTGAGG CC^T[ACGT]AGG
DraI 2681 TTTAAA TTT^AAA
Eco47III 435 AGCGCT AGC^GCT
Eco81I 1130 CCTGAGG CC^T[ACGT]AGG
HincII 2729 GTCAAC GT[CT]^[GA]AC
HindII 2729 GTCAAC GT[CT]^[GA]AC
MflI 75 GGATCC [GA]^GATC[CT]
MroI 991 TCCGGA T^CCGGA
NspI 1435 ACATGT [GA]CATG^[CT]
PflFI 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3242 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1225 TCATGA T^CATGA
SacI 1844 GAGCTC GAGCT^C
ScaI 1252 AGTACT AGT^ACT
SfiI 3076 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 1402 TGTACA T^GTACA
TfiI 2249 GATTC G^A[AT]TC
Tsp509I 1564 AATT ^AATT
Tth111I 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3242 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 75 GGATCC [GA]^GATC[CT]
XmnI 1640 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 89 CGCG CG^CG
1328 CGCG
AviII 541 TGCGCA TGC^GCA
1342 TGCGCA
BbeI 451 GGCGCC GGCGC^C
1920 GGCGCC
BbsI 1334 GAAGAC GAAGAC(2/6)
1164 GTCTTC
BbvCI 3052 CCTCAGC CCTCAGC(-5/-2)
3098 CCTCAGC
BcgI 265 GCACGGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1537 GCAACCTCATCG
BlpI 705 GCTGAGC GC^T[ACGT]AGC
2309 GCTGAGC
Bpu1102I 705 GCTGAGC GC^T[ACGT]AGC
2309 GCTGAGC
BpuAI 1334 GAAGAC GAAGAC(2/6)
1164 GTCTTC
BsrDI 2297 CATTGC GCAATG(2/0)
3235 GCAATG
BssSI 483 CTCGTG CACGAG(-5/-1)
1847 CTCGTG
BstEII 278 GGTGACC G^GT[ACGT]ACC
1199 GGTCACC
BstPI 278 GGTGACC G^GT[ACGT]ACC
1199 GGTCACC
BstUI 89 CGCG CG^CG
1328 CGCG
CelII 705 GCTGAGC GC^T[ACGT]AGC
2309 GCTGAGC
DraIII 123 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2283 CACACGGTG
EagI 642 CGGCCG C^GGCCG
1889 CGGCCG
EclXI 642 CGGCCG C^GGCCG
1889 CGGCCG
Eco52I 642 CGGCCG C^GGCCG
1889 CGGCCG
EcoNI 320 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1613 CCTGATGCAGG
EcoO65I 278 GGTGACC G^GT[ACGT]ACC
1199 GGTCACC
FspI 541 TGCGCA TGC^GCA
1342 TGCGCA
KasI 451 GGCGCC G^GCGCC
1920 GGCGCC
MseI 343 TTAA T^TAA
2682 TTAA
MvnI 89 CGCG CG^CG
1328 CGCG
NaeI 28 GCCGGC GCC^GGC
202 GCCGGC
NarI 451 GGCGCC GG^CGCC
1920 GGCGCC
NgoMIV 28 GCCGGC G^CCGGC
202 GCCGGC
PaeR7I 1707 CTCGAG C^TCGAG
2442 CTCGAG
SapI 1841 GAAGAGC GCTCTTC(1/4)
575 GCTCTTC
SexAI 124 ACCTGGT A^CC[AT]GGT
1102 ACCTGGT
SfoI 451 GGCGCC GGC^GCC
1920 GGCGCC
SmlI 1707 CTCGAG C^T[CT][GA]AG
2442 CTCGAG
StuI 2503 AGGCCT AGG^CCT
2656 AGGCCT
Tru9I 343 TTAA T^TAA
2682 TTAA
XhoI 1707 CTCGAG C^TCGAG
2442 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI