################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI BbrPI BciVI BclI BfrI BglII BsaAI BsaBI BsiWI BsmBI BsmI BspDI BssHII Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 270 GACGTC GACGT^C AccI 1602 GTCTAC GT^[AC][GT]AC AccIII 991 TCCGGA T^CCGGA AcsI 1563 GAATTT [GA]^AATT[CT] AflIII 1435 ACATGT A^C[GA][CT]GT AocI 1130 CCTGAGG CC^T[ACGT]AGG Aor51HI 435 AGCGCT AGC^GCT ApaI 697 GGGCCC GGGCC^C ApoI 1563 GAATTT [GA]^AATT[CT] Asp700I 1640 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2904 CCTAGG C^CTAGG BamHI 75 GGATCC G^GATCC BglI 3077 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2904 CCTAGG C^CTAGG BsaWI 991 TCCGGA [AT]^CCGG[AT] BseAI 991 TCCGGA T^CCGGA Bsp1407I 1402 TGTACA T^GTACA BspEI 991 TCCGGA T^CCGGA BspHI 1225 TCATGA T^CATGA BspLU11I 1435 ACATGT A^CATGT BsrGI 1402 TGTACA T^GTACA BstYI 75 GGATCC [GA]^GATC[CT] Bsu36I 1130 CCTGAGG CC^T[ACGT]AGG DraI 2681 TTTAAA TTT^AAA Eco47III 435 AGCGCT AGC^GCT Eco81I 1130 CCTGAGG CC^T[ACGT]AGG HincII 2729 GTCAAC GT[CT]^[GA]AC HindII 2729 GTCAAC GT[CT]^[GA]AC MflI 75 GGATCC [GA]^GATC[CT] MroI 991 TCCGGA T^CCGGA NspI 1435 ACATGT [GA]CATG^[CT] PflFI 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3242 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1225 TCATGA T^CATGA SacI 1844 GAGCTC GAGCT^C ScaI 1252 AGTACT AGT^ACT SfiI 3076 GGCCTGGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 1402 TGTACA T^GTACA TfiI 2249 GATTC G^A[AT]TC Tsp509I 1564 AATT ^AATT Tth111I 1101 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3242 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 75 GGATCC [GA]^GATC[CT] XmnI 1640 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AccII 89 CGCG CG^CG 1328 CGCG AviII 541 TGCGCA TGC^GCA 1342 TGCGCA BbeI 451 GGCGCC GGCGC^C 1920 GGCGCC BbsI 1334 GAAGAC GAAGAC(2/6) 1164 GTCTTC BbvCI 3052 CCTCAGC CCTCAGC(-5/-2) 3098 CCTCAGC BcgI 265 GCACGGACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1537 GCAACCTCATCG BlpI 705 GCTGAGC GC^T[ACGT]AGC 2309 GCTGAGC Bpu1102I 705 GCTGAGC GC^T[ACGT]AGC 2309 GCTGAGC BpuAI 1334 GAAGAC GAAGAC(2/6) 1164 GTCTTC BsrDI 2297 CATTGC GCAATG(2/0) 3235 GCAATG BssSI 483 CTCGTG CACGAG(-5/-1) 1847 CTCGTG BstEII 278 GGTGACC G^GT[ACGT]ACC 1199 GGTCACC BstPI 278 GGTGACC G^GT[ACGT]ACC 1199 GGTCACC BstUI 89 CGCG CG^CG 1328 CGCG CelII 705 GCTGAGC GC^T[ACGT]AGC 2309 GCTGAGC DraIII 123 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 2283 CACACGGTG EagI 642 CGGCCG C^GGCCG 1889 CGGCCG EclXI 642 CGGCCG C^GGCCG 1889 CGGCCG Eco52I 642 CGGCCG C^GGCCG 1889 CGGCCG EcoNI 320 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1613 CCTGATGCAGG EcoO65I 278 GGTGACC G^GT[ACGT]ACC 1199 GGTCACC FspI 541 TGCGCA TGC^GCA 1342 TGCGCA KasI 451 GGCGCC G^GCGCC 1920 GGCGCC MseI 343 TTAA T^TAA 2682 TTAA MvnI 89 CGCG CG^CG 1328 CGCG NaeI 28 GCCGGC GCC^GGC 202 GCCGGC NarI 451 GGCGCC GG^CGCC 1920 GGCGCC NgoMIV 28 GCCGGC G^CCGGC 202 GCCGGC PaeR7I 1707 CTCGAG C^TCGAG 2442 CTCGAG SapI 1841 GAAGAGC GCTCTTC(1/4) 575 GCTCTTC SexAI 124 ACCTGGT A^CC[AT]GGT 1102 ACCTGGT SfoI 451 GGCGCC GGC^GCC 1920 GGCGCC SmlI 1707 CTCGAG C^T[CT][GA]AG 2442 CTCGAG StuI 2503 AGGCCT AGG^CCT 2656 AGGCCT Tru9I 343 TTAA T^TAA 2682 TTAA XhoI 1707 CTCGAG C^TCGAG 2442 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI