##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

Acc65I      AclI        AflII       AgeI        AhdI        
Alw44I      ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       BbrPI       BciVI       BclI        
BfrI        BglII       BsaAI       BsaBI       BsiWI       
BsmBI       BsmI        BspDI       BssHII      Bst1107I    
BstAPI      BstBI       BstZ17I     ClaI        CpoI        
DrdI        Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AatII         270  GACGTC               GACGT^C
AccI         1602  GTCTAC               GT^[AC][GT]AC
AccIII        991  TCCGGA               T^CCGGA
AcsI         1563  GAATTT               [GA]^AATT[CT]
AflIII       1435  ACATGT               A^C[GA][CT]GT
AocI         1130  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       435  AGCGCT               AGC^GCT
ApaI          697  GGGCCC               GGGCC^C
ApoI         1563  GAATTT               [GA]^AATT[CT]
Asp700I      1640  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1101  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        2904  CCTAGG               C^CTAGG
BamHI          75  GGATCC               G^GATCC
BglI         3077  GCCTGGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2904  CCTAGG               C^CTAGG
BsaWI         991  TCCGGA               [AT]^CCGG[AT]
BseAI         991  TCCGGA               T^CCGGA
Bsp1407I     1402  TGTACA               T^GTACA
BspEI         991  TCCGGA               T^CCGGA
BspHI        1225  TCATGA               T^CATGA
BspLU11I     1435  ACATGT               A^CATGT
BsrGI        1402  TGTACA               T^GTACA
BstYI          75  GGATCC               [GA]^GATC[CT]
Bsu36I       1130  CCTGAGG              CC^T[ACGT]AGG
DraI         2681  TTTAAA               TTT^AAA
Eco47III      435  AGCGCT               AGC^GCT
Eco81I       1130  CCTGAGG              CC^T[ACGT]AGG
HincII       2729  GTCAAC               GT[CT]^[GA]AC
HindII       2729  GTCAAC               GT[CT]^[GA]AC
MflI           75  GGATCC               [GA]^GATC[CT]
MroI          991  TCCGGA               T^CCGGA
NspI         1435  ACATGT               [GA]CATG^[CT]
PflFI        1101  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3242  CCAGGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1225  TCATGA               T^CATGA
SacI         1844  GAGCTC               GAGCT^C
ScaI         1252  AGTACT               AGT^ACT
SfiI         3076  GGCCTGGTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        1402  TGTACA               T^GTACA
TfiI         2249  GATTC                G^A[AT]TC
Tsp509I      1564  AATT                 ^AATT
Tth111I      1101  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3242  CCAGGGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII          75  GGATCC               [GA]^GATC[CT]
XmnI         1640  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccII          89  CGCG                 CG^CG
             1328  CGCG                
AviII         541  TGCGCA               TGC^GCA
             1342  TGCGCA              
BbeI          451  GGCGCC               GGCGC^C
             1920  GGCGCC              
BbsI         1334  GAAGAC               GAAGAC(2/6)
             1164  GTCTTC              
BbvCI        3052  CCTCAGC              CCTCAGC(-5/-2)
             3098  CCTCAGC             
BcgI          265  GCACGGACGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1537  GCAACCTCATCG        
BlpI          705  GCTGAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
Bpu1102I      705  GCTGAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
BpuAI        1334  GAAGAC               GAAGAC(2/6)
             1164  GTCTTC              
BsrDI        2297  CATTGC               GCAATG(2/0)
             3235  GCAATG              
BssSI         483  CTCGTG               CACGAG(-5/-1)
             1847  CTCGTG              
BstEII        278  GGTGACC              G^GT[ACGT]ACC
             1199  GGTCACC             
BstPI         278  GGTGACC              G^GT[ACGT]ACC
             1199  GGTCACC             
BstUI          89  CGCG                 CG^CG
             1328  CGCG                
CelII         705  GCTGAGC              GC^T[ACGT]AGC
             2309  GCTGAGC             
DraIII        123  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2283  CACACGGTG           
EagI          642  CGGCCG               C^GGCCG
             1889  CGGCCG              
EclXI         642  CGGCCG               C^GGCCG
             1889  CGGCCG              
Eco52I        642  CGGCCG               C^GGCCG
             1889  CGGCCG              
EcoNI         320  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1613  CCTGATGCAGG         
EcoO65I       278  GGTGACC              G^GT[ACGT]ACC
             1199  GGTCACC             
FspI          541  TGCGCA               TGC^GCA
             1342  TGCGCA              
KasI          451  GGCGCC               G^GCGCC
             1920  GGCGCC              
MseI          343  TTAA                 T^TAA
             2682  TTAA                
MvnI           89  CGCG                 CG^CG
             1328  CGCG                
NaeI           28  GCCGGC               GCC^GGC
              202  GCCGGC              
NarI          451  GGCGCC               GG^CGCC
             1920  GGCGCC              
NgoMIV         28  GCCGGC               G^CCGGC
              202  GCCGGC              
PaeR7I       1707  CTCGAG               C^TCGAG
             2442  CTCGAG              
SapI         1841  GAAGAGC              GCTCTTC(1/4)
              575  GCTCTTC             
SexAI         124  ACCTGGT              A^CC[AT]GGT
             1102  ACCTGGT             
SfoI          451  GGCGCC               GGC^GCC
             1920  GGCGCC              
SmlI         1707  CTCGAG               C^T[CT][GA]AG
             2442  CTCGAG              
StuI         2503  AGGCCT               AGG^CCT
             2656  AGGCCT              
Tru9I         343  TTAA                 T^TAA
             2682  TTAA                
XhoI         1707  CTCGAG               C^TCGAG
             2442  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     XcmI