##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
BamHI BbeI BbrPI BcgI BciVI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssSI Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI FspI HgaI Hin1I
KasI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2824 GGTACC G^GTACC
Asp718I 2824 GGTACC G^GTACC
AvrII 1712 CCTAGG C^CTAGG
BalI 2479 TGGCCA TGG^CCA
BbvCI 225 CCTCAGC CCTCAGC(-5/-2)
BclI 812 TGATCA T^GATCA
BglI 22 GCCTCGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 614 AGATCT A^GATCT
BlnI 1712 CCTAGG C^CTAGG
BsaAI 2083 CACGTA [CT]AC^GT[GA]
BsaBI 2985 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 982 TGTACA T^GTACA
BspHI 2458 TCATGA T^CATGA
BspMI 750 GCAGGT ACCTGC(4/8)
BsrGI 982 TGTACA T^GTACA
BssHII 82 GCGCGC G^CGCGC
BstEII 3287 GGTGACC G^GT[ACGT]ACC
BstPI 3287 GGTGACC G^GT[ACGT]ACC
EaeI 2479 TGGCCA [CT]^GGCC[GA]
EcoO65I 3287 GGTGACC G^GT[ACGT]ACC
EcoRI 3230 GAATTC G^AATTC
EcoRV 2898 GATATC GAT^ATC
EcoT22I 2717 ATGCAT ATGCA^T
FbaI 812 TGATCA T^GATCA
HaeII 48 GGCGCT [GA]GCGC^[CT]
HindIII 630 AAGCTT A^AGCTT
HpaI 1827 GTTAAC GTT^AAC
KpnI 2824 GGTACC GGTAC^C
KspI 5 CCGCGG CCGC^GG
MamI 2985 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2479 TGGCCA TGG^CCA
MscI 2479 TGGCCA TGG^CCA
NcoI 3292 CCATGG C^CATGG
NdeI 2028 CATATG CA^TATG
NsiI 2717 ATGCAT ATGCA^T
PpuMI 3769 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1974 GACCGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 865 CTGCAG CTGCA^G
PvuII 3655 CAGCTG CAG^CTG
RcaI 2458 TCATGA T^CATGA
SacI 1238 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SapI 1878 GAAGAGC GCTCTTC(1/4)
SspBI 982 TGTACA T^GTACA
SwaI 4326 ATTTAAAT ATTT^AAAT
TaqI 1988 TCGA T^CGA
TthHB8I 1988 TCGA T^CGA
XcmI 1732 CCATTGCATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 963 CTTAAG C^TTAAG
2319 CTTAAG
AlwI 3384 GATCC GGATC(4/5)
2342 GGATC
AlwNI 878 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1222 CAGGTTCTG
AspHI 1238 GAGCTC G[AT]GC[AT]^C
1854 GTGCTC
AvaI 17 CTCGGG C^[CT]CG[GA]G
2390 CTCGGG
BanII 1238 GAGCTC G[GA]GC[CT]^C
2045 GGGCTC
BcnI 98 CCCGG CC^[GC]GG
1567 CCGGG
BfrI 963 CTTAAG C^TTAAG
2319 CTTAAG
BlpI 627 GCTAAGC GC^T[ACGT]AGC
3807 GCTAAGC
Bpu1102I 627 GCTAAGC GC^T[ACGT]AGC
3807 GCTAAGC
BsgI 1032 GTGCAG GTGCAG(16/14)
2115 GTGCAG
BsiHKAI 1238 GAGCTC G[AT]GC[AT]^C
1854 GTGCTC
BsoBI 17 CTCGGG C^[CT]CG[GA]G
2390 CTCGGG
BstXI 2776 CCATGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3026 CCATAGAGTTGG
BstYI 614 AGATCT [GA]^GATC[CT]
3383 AGATCC
CelII 627 GCTAAGC GC^T[ACGT]AGC
3807 GCTAAGC
DraII 1772 AGGGCCT [GA]G^G[ACGT]CC[CT]
3769 AGGTCCT
DraIII 1045 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
3737 CACAAGGTG
EcoO109I 1772 AGGGCCT [GA]G^G[ACGT]CC[CT]
3769 AGGTCCT
MfeI 1327 CAATTG C^AATTG
2466 CAATTG
MflI 614 AGATCT [GA]^GATC[CT]
3383 AGATCC
MspA1I 5 CCGCGG C[AC]G^C[GT]G
3655 CAGCTG
MunI 1327 CAATTG C^AATTG
2466 CAATTG
NciI 98 CCCGG CC^[GC]GG
1567 CCGGG
PflMI 1143 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3365 CCACTAACTGG
SfcI 865 CTGCAG C^T[GA][CT]AG
1256 CTATAG
Van91I 1143 CCACAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3365 CCACTAACTGG
XhoII 614 AGATCT [GA]^GATC[CT]
3383 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AocI ApoI AseI AsnI
Asp700I AvaII BanI BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DsaI EarI Eco81I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI