################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AgeI Alw44I ApaLI AscI Asp718I AviII AvrII BbeI BbrPI BcgI BfrI BglI BlnI BsaAI BsaHI BsiEI BsiWI BsmBI BspDI BspHI BspLU11I BsrFI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfoI SgrAI SmaI SnaBI SphI StuI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 443 GTCGAC GT^[AC][GT]AC AccIII 2679 TCCGGA T^CCGGA AflIII 3289 ACACGT A^C[GA][CT]GT AhdI 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2726 CCTGAGG CC^T[ACGT]AGG Aor51HI 3150 AGCGCT AGC^GCT AseI 803 ATTAAT AT^TAAT AsnI 803 ATTAAT AT^TAAT AspEI 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 828 GGATCC G^GATCC BbvCI 3275 CCTCAGC CCTCAGC(-5/-2) BciVI 2971 GTATCC GTATCC(6/5) BseAI 2679 TCCGGA T^CCGGA BspEI 2679 TCCGGA T^CCGGA BsrDI 2535 GCAATG GCAATG(2/0) BstAPI 1932 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 2726 CCTGAGG CC^T[ACGT]AGG DraIII 2189 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 2543 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3150 AGCGCT AGC^GCT Eco81I 2726 CCTGAGG CC^T[ACGT]AGG EcoRI 3955 GAATTC G^AATTC MroI 2679 TCCGGA T^CCGGA NheI 2333 GCTAGC G^CTAGC PshBI 803 ATTAAT AT^TAAT SacI 3634 GAGCTC GAGCT^C SalI 443 GTCGAC G^TCGAC SapI 3002 GAAGAGC GCTCTTC(1/4) SbfI 2563 CCTGCAGG CCTGCA^GG SexAI 914 ACCAGGT A^CC[AT]GGT SmlI 998 CTCAAG C^T[CT][GA]AG SpeI 242 ACTAGT A^CTAGT Sse8387I 2563 CCTGCAGG CCTGCA^GG SspI 1114 AATATT AAT^ATT XbaI 1258 TCTAGA T^CTAGA XcmI 2976 CCAATGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 3191 AAATTC [GA]^AATT[CT] 3955 GAATTC ApaI 2088 GGGCCC GGGCC^C 3448 GGGCCC ApoI 3191 AAATTC [GA]^AATT[CT] 3955 GAATTC Asp700I 224 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 620 GAACGACTTC BclI 1044 TGATCA T^GATCA 2203 TGATCA BsaBI 545 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1339 GATGTCTATC Bsp1407I 298 TGTACA T^GTACA 1707 TGTACA BsrGI 298 TGTACA T^GTACA 1707 TGTACA BssSI 662 CACGAG CACGAG(-5/-1) 452 CTCGTG BstBI 247 TTCGAA TT^CGAA 627 TTCGAA FbaI 1044 TGATCA T^GATCA 2203 TGATCA HaeII 25 GGCGCT [GA]GCGC^[CT] 3150 AGCGCT HgaI 3179 GACGC GACGC(5/10) 3243 GACGC MamI 545 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1339 GATGTCTATC NspI 1185 GCATGT [GA]CATG^[CT] 3371 ACATGC NspV 247 TTCGAA TT^CGAA 627 TTCGAA PpuMI 2488 GGGTCCC [GA]G^G[AT]CC[CT] 2808 AGGACCC SfuI 247 TTCGAA TT^CGAA 627 TTCGAA SspBI 298 TGTACA T^GTACA 1707 TGTACA XmnI 224 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 620 GAACGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AspI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PflFI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XhoII