##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I ApaLI AscI
Asp718I AviII AvrII BbeI BbrPI
BcgI BfrI BglI BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI BspDI
BspHI BspLU11I BsrFI BssHII Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI Hin1I HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
ScaI SfiI SfoI SgrAI SmaI
SnaBI SphI StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 443 GTCGAC GT^[AC][GT]AC
AccIII 2679 TCCGGA T^CCGGA
AflIII 3289 ACACGT A^C[GA][CT]GT
AhdI 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2726 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3150 AGCGCT AGC^GCT
AseI 803 ATTAAT AT^TAAT
AsnI 803 ATTAAT AT^TAAT
AspEI 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 828 GGATCC G^GATCC
BbvCI 3275 CCTCAGC CCTCAGC(-5/-2)
BciVI 2971 GTATCC GTATCC(6/5)
BseAI 2679 TCCGGA T^CCGGA
BspEI 2679 TCCGGA T^CCGGA
BsrDI 2535 GCAATG GCAATG(2/0)
BstAPI 1932 GCATGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2726 CCTGAGG CC^T[ACGT]AGG
DraIII 2189 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2543 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1412 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3150 AGCGCT AGC^GCT
Eco81I 2726 CCTGAGG CC^T[ACGT]AGG
EcoRI 3955 GAATTC G^AATTC
MroI 2679 TCCGGA T^CCGGA
NheI 2333 GCTAGC G^CTAGC
PshBI 803 ATTAAT AT^TAAT
SacI 3634 GAGCTC GAGCT^C
SalI 443 GTCGAC G^TCGAC
SapI 3002 GAAGAGC GCTCTTC(1/4)
SbfI 2563 CCTGCAGG CCTGCA^GG
SexAI 914 ACCAGGT A^CC[AT]GGT
SmlI 998 CTCAAG C^T[CT][GA]AG
SpeI 242 ACTAGT A^CTAGT
Sse8387I 2563 CCTGCAGG CCTGCA^GG
SspI 1114 AATATT AAT^ATT
XbaI 1258 TCTAGA T^CTAGA
XcmI 2976 CCAATGCTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 3191 AAATTC [GA]^AATT[CT]
3955 GAATTC
ApaI 2088 GGGCCC GGGCC^C
3448 GGGCCC
ApoI 3191 AAATTC [GA]^AATT[CT]
3955 GAATTC
Asp700I 224 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
620 GAACGACTTC
BclI 1044 TGATCA T^GATCA
2203 TGATCA
BsaBI 545 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1339 GATGTCTATC
Bsp1407I 298 TGTACA T^GTACA
1707 TGTACA
BsrGI 298 TGTACA T^GTACA
1707 TGTACA
BssSI 662 CACGAG CACGAG(-5/-1)
452 CTCGTG
BstBI 247 TTCGAA TT^CGAA
627 TTCGAA
FbaI 1044 TGATCA T^GATCA
2203 TGATCA
HaeII 25 GGCGCT [GA]GCGC^[CT]
3150 AGCGCT
HgaI 3179 GACGC GACGC(5/10)
3243 GACGC
MamI 545 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1339 GATGTCTATC
NspI 1185 GCATGT [GA]CATG^[CT]
3371 ACATGC
NspV 247 TTCGAA TT^CGAA
627 TTCGAA
PpuMI 2488 GGGTCCC [GA]G^G[AT]CC[CT]
2808 AGGACCC
SfuI 247 TTCGAA TT^CGAA
627 TTCGAA
SspBI 298 TGTACA T^GTACA
1707 TGTACA
XmnI 224 GAACCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
620 GAACGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoII