##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AscI Asp718I
AviII AvrII BbeI BbrPI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI BstBI BstUI
CelII Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco52I EcoRI
EcoT22I FseI FspI HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcsI 328 AAATTC [GA]^AATT[CT]
Alw44I 1846 GTGCAC G^TGCAC
Aor51HI 156 AGCGCT AGC^GCT
ApaLI 1846 GTGCAC G^TGCAC
ApoI 328 AAATTC [GA]^AATT[CT]
Asp700I 721 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1648 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2331 TGGCCA TGG^CCA
BbvCI 777 CCTCAGC CCTCAGC(-5/-2)
BcgI 621 CGAAGAGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 981 TGATCA T^GATCA
BglI 2488 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 871 TACGTG [CT]AC^GT[GA]
BsaI 1835 GGTCTC GGTCTC(1/5)
BsiWI 1689 CGTACG C^GTACG
BsmBI 2703 CGTCTC CGTCTC(1/5)
BspMI 609 ACCTGC ACCTGC(4/8)
BsrBI 1541 GAGCGG CCGCTC(-3/-3)
Bst1107I 2711 GTATAC GTA^TAC
BstAPI 2746 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1856 GGTGACC G^GT[ACGT]ACC
BstPI 1856 GGTGACC G^GT[ACGT]ACC
BstXI 263 CCAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2711 GTATAC GTA^TAC
DraI 1370 TTTAAA TTT^AAA
DsaI 975 CCACGG C^C[GA][CT]GG
Eco47III 156 AGCGCT AGC^GCT
EcoNI 38 CCTGACGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1856 GGTGACC G^GT[ACGT]ACC
EcoRV 1332 GATATC GAT^ATC
FbaI 981 TGATCA T^GATCA
HincII 1911 GTTGAC GT[CT]^[GA]AC
HindII 1911 GTTGAC GT[CT]^[GA]AC
MfeI 783 CAATTG C^AATTG
MluNI 2331 TGGCCA TGG^CCA
MscI 2331 TGGCCA TGG^CCA
MunI 783 CAATTG C^AATTG
PflFI 1648 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1926 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 959 GAGCTC GAGCT^C
SexAI 1931 ACCTGGT A^CC[AT]GGT
SspI 1811 AATATT AAT^ATT
TaqI 620 TCGA T^CGA
Tth111I 1648 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 620 TCGA T^CGA
Van91I 1926 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 721 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 100 GTAGAC GT^[AC][GT]AC
2711 GTATAC
AflIII 723 ACGTGT A^C[GA][CT]GT
872 ACGTGT
AhdI 1520 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2415 GACTTCCGGTC
AlwNI 1927 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
2585 CAGGACCTG
AocI 1851 CCTCAGG CC^T[ACGT]AGG
1945 CCTTAGG
ApaI 127 GGGCCC GGGCC^C
1824 GGGCCC
AseI 926 ATTAAT AT^TAAT
2732 ATTAAT
AsnI 926 ATTAAT AT^TAAT
2732 ATTAAT
AspEI 1520 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2415 GACTTCCGGTC
AvaI 1077 CTCGGG C^[CT]CG[GA]G
1171 CTCGGG
BamHI 144 GGATCC G^GATCC
301 GGATCC
BanI 1460 GGCACC G^G[CT][GA]CC
2362 GGCACC
BbsI 2044 GAAGAC GAAGAC(2/6)
2165 GAAGAC
BpuAI 2044 GAAGAC GAAGAC(2/6)
2165 GAAGAC
BsaBI 145 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1285 GATAGCCATC
BsaWI 525 ACCGGA [AT]^CCGG[AT]
2419 TCCGGT
BsoBI 1077 CTCGGG C^[CT]CG[GA]G
1171 CTCGGG
BsrDI 1231 CATTGC GCAATG(2/0)
1704 CATTGC
BstYI 144 GGATCC [GA]^GATC[CT]
301 GGATCC
Bsu36I 1851 CCTCAGG CC^T[ACGT]AGG
1945 CCTTAGG
EaeI 1544 CGGCCA [CT]^GGCC[GA]
2331 TGGCCA
Eam1105I 1520 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2415 GACTTCCGGTC
Eco81I 1851 CCTCAGG CC^T[ACGT]AGG
1945 CCTTAGG
EcoT14I 231 CCAAGG C^C[AT][AT]GG
603 CCAAGG
FauI 1381 CCCGC CCCGC(4/6)
2397 CCCGC
MamI 145 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1285 GATAGCCATC
MflI 144 GGATCC [GA]^GATC[CT]
301 GGATCC
MslI 1953 CACTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2681 CATATAAGTG
PshBI 926 ATTAAT AT^TAAT
2732 ATTAAT
PvuII 614 CAGCTG CAG^CTG
2049 CAGCTG
SapI 2242 GAAGAGC GCTCTTC(1/4)
237 GCTCTTC
SmlI 1574 CTCAAG C^T[CT][GA]AG
2741 CTTGAG
StyI 231 CCAAGG C^C[AT][AT]GG
603 CCAAGG
XcmI 1260 CCAGCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2041 CCAGAAGACAGCTGG
XhoII 144 GGATCC [GA]^GATC[CT]
301 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI