################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BciVI BcnI BglI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstUI Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoT22I FauI FseI FspI HapII HgaI Hin1I HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 143 GTATAC GT^[AC][GT]AC AflII 2992 CTTAAG C^TTAAG AlwI 1220 GATCC GGATC(4/5) AlwNI 2141 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 2064 CCTAAGG CC^T[ACGT]AGG AvrII 2983 CCTAGG C^CTAGG BalI 1633 TGGCCA TGG^CCA BanII 1456 GGGCTC G[GA]GC[CT]^C BbsI 774 GTCTTC GAAGAC(2/6) BcgI 670 CGATGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 538 TGATCA T^GATCA BfrI 2992 CTTAAG C^TTAAG BglII 3093 AGATCT A^GATCT BlnI 2983 CCTAGG C^CTAGG BlpI 408 GCTTAGC GC^T[ACGT]AGC Bpu1102I 408 GCTTAGC GC^T[ACGT]AGC BpuAI 774 GTCTTC GAAGAC(2/6) BsaI 704 GAGACC GGTCTC(1/5) BsiWI 2735 CGTACG C^GTACG BspHI 946 TCATGA T^CATGA BspLU11I 2727 ACATGT A^CATGT Bst1107I 143 GTATAC GTA^TAC BstEII 1523 GGTTACC G^GT[ACGT]ACC BstPI 1523 GGTTACC G^GT[ACGT]ACC BstXI 1566 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 143 GTATAC GTA^TAC Bsu36I 2064 CCTAAGG CC^T[ACGT]AGG CelII 408 GCTTAGC GC^T[ACGT]AGC CfoI 1996 GCGC GCG^C DraIII 2241 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2064 CCTAAGG CC^T[ACGT]AGG EcoNI 2064 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1523 GGTTACC G^GT[ACGT]ACC EcoRI 1066 GAATTC G^AATTC EcoRV 2600 GATATC GAT^ATC FbaI 538 TGATCA T^GATCA HaeII 1995 GGCGCT [GA]GCGC^[CT] HhaI 1996 GCGC GCG^C HinP1I 1996 GCGC G^CGC HindIII 2624 AAGCTT A^AGCTT MfeI 168 CAATTG C^AATTG MluNI 1633 TGGCCA TGG^CCA MscI 1633 TGGCCA TGG^CCA MunI 168 CAATTG C^AATTG PflMI 87 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 946 TCATGA T^CATGA SexAI 1059 ACCAGGT A^CC[AT]GGT SphI 1555 GCATGC GCATG^C SwaI 1288 ATTTAAAT ATTT^AAAT Van91I 87 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 710 CCAGATCGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AflIII 2245 ACGTGT A^C[GA][CT]GT 2727 ACATGT Asp700I 2364 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2841 GAATCATTTC BsmFI 723 GGGAC GGGAC(10/14) 3332 GTCCC BsmI 2383 GCATTC GAATGC(1/-1) 3356 GCATTC BspMI 1468 GCAGGT ACCTGC(4/8) 2070 GCAGGT BstYI 1219 AGATCC [GA]^GATC[CT] 3093 AGATCT DsaI 1566 CCATGG C^C[GA][CT]GG 3335 CCATGG HpaI 1129 GTTAAC GTT^AAC 1753 GTTAAC MflI 1219 AGATCC [GA]^GATC[CT] 3093 AGATCT MspA1I 414 CAGCTG C[AC]G^C[GT]G 2412 CAGCTG NcoI 1566 CCATGG C^CATGG 3335 CCATGG NspI 1555 GCATGC [GA]CATG^[CT] 2727 ACATGT PpuMI 723 GGGACCT [GA]G^G[AT]CC[CT] 2979 AGGTCCT PvuII 414 CAGCTG CAG^CTG 2412 CAGCTG ScaI 568 AGTACT AGT^ACT 3388 AGTACT SmlI 609 CTCAAG C^T[CT][GA]AG 2992 CTTAAG XbaI 1801 TCTAGA T^CTAGA 1937 TCTAGA XhoII 1219 AGATCC [GA]^GATC[CT] 3093 AGATCT XmnI 2364 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2841 GAATCATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I