##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
BamHI BanI BbeI BbrPI BbvCI
BciVI BcnI BglI BpmI BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiEI BsmBI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstUI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoT22I FauI FseI
FspI HapII HgaI Hin1I HpaII
KasI KpnI KspI MamI MluI
MroI MspI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 143 GTATAC GT^[AC][GT]AC
AflII 2992 CTTAAG C^TTAAG
AlwI 1220 GATCC GGATC(4/5)
AlwNI 2141 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2064 CCTAAGG CC^T[ACGT]AGG
AvrII 2983 CCTAGG C^CTAGG
BalI 1633 TGGCCA TGG^CCA
BanII 1456 GGGCTC G[GA]GC[CT]^C
BbsI 774 GTCTTC GAAGAC(2/6)
BcgI 670 CGATGACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 538 TGATCA T^GATCA
BfrI 2992 CTTAAG C^TTAAG
BglII 3093 AGATCT A^GATCT
BlnI 2983 CCTAGG C^CTAGG
BlpI 408 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 408 GCTTAGC GC^T[ACGT]AGC
BpuAI 774 GTCTTC GAAGAC(2/6)
BsaI 704 GAGACC GGTCTC(1/5)
BsiWI 2735 CGTACG C^GTACG
BspHI 946 TCATGA T^CATGA
BspLU11I 2727 ACATGT A^CATGT
Bst1107I 143 GTATAC GTA^TAC
BstEII 1523 GGTTACC G^GT[ACGT]ACC
BstPI 1523 GGTTACC G^GT[ACGT]ACC
BstXI 1566 CCATGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 143 GTATAC GTA^TAC
Bsu36I 2064 CCTAAGG CC^T[ACGT]AGG
CelII 408 GCTTAGC GC^T[ACGT]AGC
CfoI 1996 GCGC GCG^C
DraIII 2241 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2064 CCTAAGG CC^T[ACGT]AGG
EcoNI 2064 CCTAAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1523 GGTTACC G^GT[ACGT]ACC
EcoRI 1066 GAATTC G^AATTC
EcoRV 2600 GATATC GAT^ATC
FbaI 538 TGATCA T^GATCA
HaeII 1995 GGCGCT [GA]GCGC^[CT]
HhaI 1996 GCGC GCG^C
HinP1I 1996 GCGC G^CGC
HindIII 2624 AAGCTT A^AGCTT
MfeI 168 CAATTG C^AATTG
MluNI 1633 TGGCCA TGG^CCA
MscI 1633 TGGCCA TGG^CCA
MunI 168 CAATTG C^AATTG
PflMI 87 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 946 TCATGA T^CATGA
SexAI 1059 ACCAGGT A^CC[AT]GGT
SphI 1555 GCATGC GCATG^C
SwaI 1288 ATTTAAAT ATTT^AAAT
Van91I 87 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 710 CCAGATCGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 2245 ACGTGT A^C[GA][CT]GT
2727 ACATGT
Asp700I 2364 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2841 GAATCATTTC
BsmFI 723 GGGAC GGGAC(10/14)
3332 GTCCC
BsmI 2383 GCATTC GAATGC(1/-1)
3356 GCATTC
BspMI 1468 GCAGGT ACCTGC(4/8)
2070 GCAGGT
BstYI 1219 AGATCC [GA]^GATC[CT]
3093 AGATCT
DsaI 1566 CCATGG C^C[GA][CT]GG
3335 CCATGG
HpaI 1129 GTTAAC GTT^AAC
1753 GTTAAC
MflI 1219 AGATCC [GA]^GATC[CT]
3093 AGATCT
MspA1I 414 CAGCTG C[AC]G^C[GT]G
2412 CAGCTG
NcoI 1566 CCATGG C^CATGG
3335 CCATGG
NspI 1555 GCATGC [GA]CATG^[CT]
2727 ACATGT
PpuMI 723 GGGACCT [GA]G^G[AT]CC[CT]
2979 AGGTCCT
PvuII 414 CAGCTG CAG^CTG
2412 CAGCTG
ScaI 568 AGTACT AGT^ACT
3388 AGTACT
SmlI 609 CTCAAG C^T[CT][GA]AG
2992 CTTAAG
XbaI 1801 TCTAGA T^CTAGA
1937 TCTAGA
XhoII 1219 AGATCC [GA]^GATC[CT]
3093 AGATCT
XmnI 2364 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2841 GAATCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BbvI BfaI BmyI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I