##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AviII AvrII BalI
BamHI BglII BlnI BsaBI BseAI
BsiEI BsiWI Bsp1407I BspEI BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SmaI SnaBI Sse8387I SspBI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2323 GACGTC GACGT^C
AccI 3207 GTAGAC GT^[AC][GT]AC
AflII 2803 CTTAAG C^TTAAG
ApaI 2341 GGGCCC GGGCC^C
Asp700I 1995 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2518 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2990 CTCGGG C^[CT]CG[GA]G
BbeI 1166 GGCGCC GGCGC^C
BbrPI 4023 CACGTG CAC^GTG
BfrI 2803 CTTAAG C^TTAAG
BglI 2334 GCCCTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4023 CACGTG [CT]AC^GT[GA]
BsaWI 1393 ACCGGA [AT]^CCGG[AT]
BsmBI 466 GAGACG CGTCTC(1/5)
BsoBI 2990 CTCGGG C^[CT]CG[GA]G
BspDI 919 ATCGAT AT^CGAT
ClaI 919 ATCGAT AT^CGAT
DraIII 1183 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 870 GACAGAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1955 TGGCCG [CT]^GGCC[GA]
EcoNI 3082 CCTAATCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1999 GAATTC G^AATTC
HgaI 811 GCGTC GACGC(5/10)
HincII 59 GTCAAC GT[CT]^[GA]AC
HindII 59 GTCAAC GT[CT]^[GA]AC
KasI 1166 GGCGCC G^GCGCC
NaeI 986 GCCGGC GCC^GGC
NarI 1166 GGCGCC GG^CGCC
NcoI 3044 CCATGG C^CATGG
NgoMIV 986 GCCGGC G^CCGGC
PflFI 2518 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4023 CACGTG CAC^GTG
PmlI 4023 CACGTG CAC^GTG
SexAI 2519 ACCTGGT A^CC[AT]GGT
SfoI 1166 GGCGCC GGC^GCC
SpeI 3417 ACTAGT A^CTAGT
SphI 3615 GCATGC GCATG^C
StuI 2456 AGGCCT AGG^CCT
Tth111I 2518 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3860 TCTAGA T^CTAGA
XmnI 1995 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 1166 GGCGCC G[GA]^CG[CT]C
2323 GACGTC
Alw44I 906 GTGCAC G^TGCAC
3623 GTGCAC
AocI 2488 CCTCAGG CC^T[ACGT]AGG
2635 CCTCAGG
ApaLI 906 GTGCAC G^TGCAC
3623 GTGCAC
BcgI 4 GCAGGCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1140 GCACAACCCTCG
BclI 539 TGATCA T^GATCA
599 TGATCA
BcnI 2126 CCCGG CC^[GC]GG
2627 CCGGG
BlpI 1348 GCTCAGC GC^T[ACGT]AGC
3870 GCTCAGC
Bpu1102I 1348 GCTCAGC GC^T[ACGT]AGC
3870 GCTCAGC
BsaHI 1166 GGCGCC G[GA]^CG[CT]C
2323 GACGTC
BsaI 389 GGTCTC GGTCTC(1/5)
2523 GGTCTC
BsmI 3838 GAATGC GAATGC(1/-1)
75 GCATTC
Bsu36I 2488 CCTCAGG CC^T[ACGT]AGG
2635 CCTCAGG
CelII 1348 GCTCAGC GC^T[ACGT]AGC
3870 GCTCAGC
Eco81I 2488 CCTCAGG CC^T[ACGT]AGG
2635 CCTCAGG
EcoT22I 120 ATGCAT ATGCA^T
1211 ATGCAT
FauI 69 CCCGC CCCGC(4/6)
948 GCGGG
FbaI 539 TGATCA T^GATCA
599 TGATCA
Hin1I 1166 GGCGCC G[GA]^CG[CT]C
2323 GACGTC
MslI 2369 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3616 CATGCTTGTG
NciI 2126 CCCGG CC^[GC]GG
2627 CCGGG
NsiI 120 ATGCAT ATGCA^T
1211 ATGCAT
PflMI 3227 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3805 CCAGAGACTGG
PpuMI 710 AGGACCT [GA]G^G[AT]CC[CT]
2630 GGGACCC
ScaI 644 AGTACT AGT^ACT
2986 AGTACT
SspI 481 AATATT AAT^ATT
2012 AATATT
Van91I 3227 CCATTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3805 CCAGAGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BciVI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII