################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AflII AflIII AgeI AocI AscI AseI AsnI AviII BalI BbeI BbrPI BbsI BcgI BclI BfrI BpuAI BsaAI BsaBI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrBI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI KasI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 591 GACGTC GACGT^C Acc65I 1181 GGTACC G^GTACC AccIII 1709 TCCGGA T^CCGGA AclI 3765 AACGTT AA^CGTT AcyI 591 GACGTC G[GA]^CG[CT]C AhdI 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 883 AGCGCT AGC^GCT ApaI 1353 GGGCCC GGGCC^C Asp718I 1181 GGTACC G^GTACC AspEI 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 2267 CCTAGG C^CTAGG BamHI 1813 GGATCC G^GATCC BbvCI 965 GCTGAGG CCTCAGC(-5/-2) BglI 1646 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2267 CCTAGG C^CTAGG BlpI 3319 GCTTAGC GC^T[ACGT]AGC Bpu1102I 3319 GCTTAGC GC^T[ACGT]AGC BsaHI 591 GACGTC G[GA]^CG[CT]C BseAI 1709 TCCGGA T^CCGGA BsmBI 2329 CGTCTC CGTCTC(1/5) BsmI 2189 GAATGC GAATGC(1/-1) BspEI 1709 TCCGGA T^CCGGA BssHII 2386 GCGCGC G^CGCGC CelII 3319 GCTTAGC GC^T[ACGT]AGC Eam1105I 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 883 AGCGCT AGC^GCT Hin1I 591 GACGTC G[GA]^CG[CT]C HpaI 1716 GTTAAC GTT^AAC KpnI 1181 GGTACC GGTAC^C MroI 1709 TCCGGA T^CCGGA NcoI 3247 CCATGG C^CATGG NspI 2949 GCATGC [GA]CATG^[CT] Psp1406I 3765 AACGTT AA^CGTT SacI 1955 GAGCTC GAGCT^C SgrAI 1904 CACCGGCG C[GA]^CCGG[CT]G SmaI 82 CCCGGG CCC^GGG SmlI 867 CTTGAG C^T[CT][GA]AG SphI 2949 GCATGC GCATG^C XmaCI 82 CCCGGG C^CCGGG XmaI 82 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 1222 GTCTAC GT^[AC][GT]AC 3606 GTAGAC AcsI 206 AAATTT [GA]^AATT[CT] 2137 AAATTC Alw44I 1983 GTGCAC G^TGCAC 2580 GTGCAC ApaLI 1983 GTGCAC G^TGCAC 2580 GTGCAC ApoI 206 AAATTT [GA]^AATT[CT] 2137 AAATTC AspI 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 491 GACGTAGTC BciVI 849 GGATAC GTATCC(6/5) 1111 GTATCC BglII 2707 AGATCT A^GATCT 3471 AGATCT BspHI 2254 TCATGA T^CATGA 3595 TCATGA BspMI 1538 GCAGGT ACCTGC(4/8) 1655 GCAGGT BsrDI 2299 GCAATG GCAATG(2/0) 3028 GCAATG BsrFI 856 ACCGGC [GA]^CCGG[CT] 1905 ACCGGC BssSI 2694 CACGAG CACGAG(-5/-1) 3171 CACGAG Cfr10I 856 ACCGGC [GA]^CCGG[CT] 1905 ACCGGC DraI 420 TTTAAA TTT^AAA 1836 TTTAAA DsaI 1093 CCGTGG C^C[GA][CT]GG 3247 CCATGG EaeI 858 CGGCCA [CT]^GGCC[GA] 1570 TGGCCG HaeII 883 AGCGCT [GA]GCGC^[CT] 2065 AGCGCC HgaI 2946 GACGC GACGC(5/10) 1604 GCGTC HincII 1716 GTTAAC GT[CT]^[GA]AC 3070 GTCAAC HindII 1716 GTTAAC GT[CT]^[GA]AC 3070 GTCAAC HindIII 2372 AAGCTT A^AGCTT 2875 AAGCTT PflFI 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 491 GACGTAGTC PflMI 1358 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3720 CCAGCCACTGG PvuII 1552 CAGCTG CAG^CTG 2798 CAGCTG RcaI 2254 TCATGA T^CATGA 3595 TCATGA ScaI 1163 AGTACT AGT^ACT 3731 AGTACT StuI 1051 AGGCCT AGG^CCT 2791 AGGCCT Tth111I 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 491 GACGTAGTC Van91I 1358 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3720 CCAGCCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI