##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AflII AflIII AgeI AocI AscI
AseI AsnI AviII BalI BbeI
BbrPI BbsI BcgI BclI BfrI
BpuAI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI KasI KspI MamI
MfeI MluI MluNI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 591 GACGTC GACGT^C
Acc65I 1181 GGTACC G^GTACC
AccIII 1709 TCCGGA T^CCGGA
AclI 3765 AACGTT AA^CGTT
AcyI 591 GACGTC G[GA]^CG[CT]C
AhdI 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 883 AGCGCT AGC^GCT
ApaI 1353 GGGCCC GGGCC^C
Asp718I 1181 GGTACC G^GTACC
AspEI 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2267 CCTAGG C^CTAGG
BamHI 1813 GGATCC G^GATCC
BbvCI 965 GCTGAGG CCTCAGC(-5/-2)
BglI 1646 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2267 CCTAGG C^CTAGG
BlpI 3319 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3319 GCTTAGC GC^T[ACGT]AGC
BsaHI 591 GACGTC G[GA]^CG[CT]C
BseAI 1709 TCCGGA T^CCGGA
BsmBI 2329 CGTCTC CGTCTC(1/5)
BsmI 2189 GAATGC GAATGC(1/-1)
BspEI 1709 TCCGGA T^CCGGA
BssHII 2386 GCGCGC G^CGCGC
CelII 3319 GCTTAGC GC^T[ACGT]AGC
Eam1105I 115 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 883 AGCGCT AGC^GCT
Hin1I 591 GACGTC G[GA]^CG[CT]C
HpaI 1716 GTTAAC GTT^AAC
KpnI 1181 GGTACC GGTAC^C
MroI 1709 TCCGGA T^CCGGA
NcoI 3247 CCATGG C^CATGG
NspI 2949 GCATGC [GA]CATG^[CT]
Psp1406I 3765 AACGTT AA^CGTT
SacI 1955 GAGCTC GAGCT^C
SgrAI 1904 CACCGGCG C[GA]^CCGG[CT]G
SmaI 82 CCCGGG CCC^GGG
SmlI 867 CTTGAG C^T[CT][GA]AG
SphI 2949 GCATGC GCATG^C
XmaCI 82 CCCGGG C^CCGGG
XmaI 82 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1222 GTCTAC GT^[AC][GT]AC
3606 GTAGAC
AcsI 206 AAATTT [GA]^AATT[CT]
2137 AAATTC
Alw44I 1983 GTGCAC G^TGCAC
2580 GTGCAC
ApaLI 1983 GTGCAC G^TGCAC
2580 GTGCAC
ApoI 206 AAATTT [GA]^AATT[CT]
2137 AAATTC
AspI 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
491 GACGTAGTC
BciVI 849 GGATAC GTATCC(6/5)
1111 GTATCC
BglII 2707 AGATCT A^GATCT
3471 AGATCT
BspHI 2254 TCATGA T^CATGA
3595 TCATGA
BspMI 1538 GCAGGT ACCTGC(4/8)
1655 GCAGGT
BsrDI 2299 GCAATG GCAATG(2/0)
3028 GCAATG
BsrFI 856 ACCGGC [GA]^CCGG[CT]
1905 ACCGGC
BssSI 2694 CACGAG CACGAG(-5/-1)
3171 CACGAG
Cfr10I 856 ACCGGC [GA]^CCGG[CT]
1905 ACCGGC
DraI 420 TTTAAA TTT^AAA
1836 TTTAAA
DsaI 1093 CCGTGG C^C[GA][CT]GG
3247 CCATGG
EaeI 858 CGGCCA [CT]^GGCC[GA]
1570 TGGCCG
HaeII 883 AGCGCT [GA]GCGC^[CT]
2065 AGCGCC
HgaI 2946 GACGC GACGC(5/10)
1604 GCGTC
HincII 1716 GTTAAC GT[CT]^[GA]AC
3070 GTCAAC
HindII 1716 GTTAAC GT[CT]^[GA]AC
3070 GTCAAC
HindIII 2372 AAGCTT A^AGCTT
2875 AAGCTT
PflFI 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
491 GACGTAGTC
PflMI 1358 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3720 CCAGCCACTGG
PvuII 1552 CAGCTG CAG^CTG
2798 CAGCTG
RcaI 2254 TCATGA T^CATGA
3595 TCATGA
ScaI 1163 AGTACT AGT^ACT
3731 AGTACT
StuI 1051 AGGCCT AGG^CCT
2791 AGGCCT
Tth111I 363 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
491 GACGTAGTC
Van91I 1358 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3720 CCAGCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Asp700I AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI