##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I BbeI BbrPI BciVI
BclI BfrI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV FbaI FseI
HaeII Hin1I HpaI KasI KpnI
KspI MfeI MroI MunI NarI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PvuI
RsrII SacII SalI SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 404 AACGTT AA^CGTT
AhdI 3838 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3071 GGGCCC GGGCC^C
Asp700I 1114 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3838 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2614 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1813 CCTAGG C^CTAGG
BbsI 1688 GTCTTC GAAGAC(2/6)
BcgI 799 CGAGATTAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 1813 CCTAGG C^CTAGG
BpuAI 1688 GTCTTC GAAGAC(2/6)
BsaBI 958 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 813 TCATGA T^CATGA
BsrDI 676 CATTGC GCAATG(2/0)
BssSI 565 CACGAG CACGAG(-5/-1)
BstEII 631 GGTGACC G^GT[ACGT]ACC
BstPI 631 GGTGACC G^GT[ACGT]ACC
BstXI 131 CCACCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 3838 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 631 GGTGACC G^GT[ACGT]ACC
EcoT22I 4177 ATGCAT ATGCA^T
MamI 958 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 144 ACGCGT A^CGCGT
MslI 298 CACGCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 4177 ATGCAT ATGCA^T
PaeR7I 3593 CTCGAG C^TCGAG
PflFI 2614 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 404 AACGTT AA^CGTT
RcaI 813 TCATGA T^CATGA
SapI 3506 GCTCTTC GCTCTTC(1/4)
SexAI 2501 ACCAGGT A^CC[AT]GGT
SfiI 1333 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1363 GCATGC GCATG^C
Tth111I 2614 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3471 TCTAGA T^CTAGA
XhoI 3593 CTCGAG C^TCGAG
XmnI 1114 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 1577 CCTCAGG CC^T[ACGT]AGG
3781 CCTTAGG
AseI 803 ATTAAT AT^TAAT
3554 ATTAAT
AsnI 803 ATTAAT AT^TAAT
3554 ATTAAT
AviII 111 TGCGCA TGC^GCA
2478 TGCGCA
BamHI 15 GGATCC G^GATCC
570 GGATCC
BbvCI 3543 GCTGAGG CCTCAGC(-5/-2)
3798 GCTGAGG
BspLU11I 1430 ACATGT A^CATGT
3894 ACATGT
BsrBI 1735 CCGCTC CCGCTC(-3/-3)
1439 GAGCGG
Bsu36I 1577 CCTCAGG CC^T[ACGT]AGG
3781 CCTTAGG
DraI 2015 TTTAAA TTT^AAA
2485 TTTAAA
DraIII 1480 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2500 CACCAGGTG
Eco81I 1577 CCTCAGG CC^T[ACGT]AGG
3781 CCTTAGG
EcoNI 1808 CCTGCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2228 CCTGTGACAGG
EcoRI 1851 GAATTC G^AATTC
4045 GAATTC
FspI 111 TGCGCA TGC^GCA
2478 TGCGCA
HindIII 3080 AAGCTT A^AGCTT
3583 AAGCTT
MaeII 405 ACGT A^CGT
2320 ACGT
NaeI 1005 GCCGGC GCC^GGC
1789 GCCGGC
NdeI 1098 CATATG CA^TATG
4081 CATATG
NgoMIV 1005 GCCGGC G^CCGGC
1789 GCCGGC
PpuMI 981 AGGACCT [GA]G^G[AT]CC[CT]
2138 GGGACCC
PshAI 2200 GACCCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2770 GACCCCCGTC
PshBI 803 ATTAAT AT^TAAT
3554 ATTAAT
SmaI 36 CCCGGG CCC^GGG
2829 CCCGGG
StuI 1356 AGGCCT AGG^CCT
1767 AGGCCT
XmaCI 36 CCCGGG C^CCGGG
2829 CCCGGG
XmaI 36 CCCGGG C^CCGGG
2829 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BglII
BmyI BpmI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII