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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I AspEI AspI AvaI
BalI BamHI BbeI BcgI BclI
BcnI BglI BlpI Bpu1102I BsaBI
BsaHI BsaI BseAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FbaI
FseI HgaI Hin1I HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 956 GGTACC G^GTACC
AccII 271 CGCG CG^CG
AflIII 3618 ACATGT A^C[GA][CT]GT
AseI 99 ATTAAT AT^TAAT
AsnI 99 ATTAAT AT^TAAT
Asp718I 956 GGTACC G^GTACC
AviII 919 TGCGCA TGC^GCA
AvrII 1217 CCTAGG C^CTAGG
BanI 956 GGTACC G^G[CT][GA]CC
BanII 1396 GGGCTC G[GA]GC[CT]^C
BbrPI 2844 CACGTG CAC^GTG
BbsI 2553 GAAGAC GAAGAC(2/6)
BbvCI 4152 GCTGAGG CCTCAGC(-5/-2)
BlnI 1217 CCTAGG C^CTAGG
BpuAI 2553 GAAGAC GAAGAC(2/6)
BsiEI 1259 CGACCG CG[GA][CT]^CG
BsmI 3493 GAATGC GAATGC(1/-1)
BspLU11I 3618 ACATGT A^CATGT
BspMI 1119 ACCTGC ACCTGC(4/8)
BstUI 271 CGCG CG^CG
DrdI 523 GACCCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 3266 CCATGG C^C[GA][CT]GG
EcoRI 3838 GAATTC G^AATTC
FauI 3023 CCCGC CCCGC(4/6)
FspI 919 TGCGCA TGC^GCA
KpnI 956 GGTACC GGTAC^C
MvnI 271 CGCG CG^CG
NcoI 3266 CCATGG C^CATGG
PflMI 1359 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2844 CACGTG CAC^GTG
PmlI 2844 CACGTG CAC^GTG
PshBI 99 ATTAAT AT^TAAT
ScaI 1198 AGTACT AGT^ACT
StuI 149 AGGCCT AGG^CCT
TaqI 2946 TCGA T^CGA
TthHB8I 2946 TCGA T^CGA
Van91I 1359 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2252 CCAACACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflII 2187 CTTAAG C^TTAAG
2775 CTTAAG
Alw44I 1115 GTGCAC G^TGCAC
2892 GTGCAC
AlwNI 1360 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
3077 CAGTCTCTG
ApaLI 1115 GTGCAC G^TGCAC
2892 GTGCAC
BciVI 1041 GGATAC GTATCC(6/5)
1253 GGATAC
BfrI 2187 CTTAAG C^TTAAG
2775 CTTAAG
BglII 2481 AGATCT A^GATCT
3903 AGATCT
BpmI 1733 CTCCAG CTGGAG(16/14)
3311 CTCCAG
BsaAI 330 CACGTA [CT]AC^GT[GA]
2844 CACGTG
BsaWI 1530 TCCGGT [AT]^CCGG[AT]
2125 ACCGGA
BseRI 2145 GAGGAG GAGGAG(10/8)
2354 GAGGAG
EcoRV 2838 GATATC GAT^ATC
3122 GATATC
HaeII 1525 GGCGCT [GA]GCGC^[CT]
2749 AGCGCC
HapII 1531 CCGG C^CGG
2126 CCGG
HpaII 1531 CCGG C^CGG
2126 CCGG
MspI 1531 CCGG C^CGG
2126 CCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BfaI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII