################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccIII AclI AcyI AgeI AhdI AocI Aor51HI ApaI AscI Asp700I AspEI AspI AvaI BalI BamHI BbeI BcgI BclI BcnI BglI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I FbaI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 956 GGTACC G^GTACC AccII 271 CGCG CG^CG AflIII 3618 ACATGT A^C[GA][CT]GT AseI 99 ATTAAT AT^TAAT AsnI 99 ATTAAT AT^TAAT Asp718I 956 GGTACC G^GTACC AviII 919 TGCGCA TGC^GCA AvrII 1217 CCTAGG C^CTAGG BanI 956 GGTACC G^G[CT][GA]CC BanII 1396 GGGCTC G[GA]GC[CT]^C BbrPI 2844 CACGTG CAC^GTG BbsI 2553 GAAGAC GAAGAC(2/6) BbvCI 4152 GCTGAGG CCTCAGC(-5/-2) BlnI 1217 CCTAGG C^CTAGG BpuAI 2553 GAAGAC GAAGAC(2/6) BsiEI 1259 CGACCG CG[GA][CT]^CG BsmI 3493 GAATGC GAATGC(1/-1) BspLU11I 3618 ACATGT A^CATGT BspMI 1119 ACCTGC ACCTGC(4/8) BstUI 271 CGCG CG^CG DrdI 523 GACCCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 3266 CCATGG C^C[GA][CT]GG EcoRI 3838 GAATTC G^AATTC FauI 3023 CCCGC CCCGC(4/6) FspI 919 TGCGCA TGC^GCA KpnI 956 GGTACC GGTAC^C MvnI 271 CGCG CG^CG NcoI 3266 CCATGG C^CATGG PflMI 1359 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2844 CACGTG CAC^GTG PmlI 2844 CACGTG CAC^GTG PshBI 99 ATTAAT AT^TAAT ScaI 1198 AGTACT AGT^ACT StuI 149 AGGCCT AGG^CCT TaqI 2946 TCGA T^CGA TthHB8I 2946 TCGA T^CGA Van91I 1359 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2252 CCAACACAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflII 2187 CTTAAG C^TTAAG 2775 CTTAAG Alw44I 1115 GTGCAC G^TGCAC 2892 GTGCAC AlwNI 1360 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG 3077 CAGTCTCTG ApaLI 1115 GTGCAC G^TGCAC 2892 GTGCAC BciVI 1041 GGATAC GTATCC(6/5) 1253 GGATAC BfrI 2187 CTTAAG C^TTAAG 2775 CTTAAG BglII 2481 AGATCT A^GATCT 3903 AGATCT BpmI 1733 CTCCAG CTGGAG(16/14) 3311 CTCCAG BsaAI 330 CACGTA [CT]AC^GT[GA] 2844 CACGTG BsaWI 1530 TCCGGT [AT]^CCGG[AT] 2125 ACCGGA BseRI 2145 GAGGAG GAGGAG(10/8) 2354 GAGGAG EcoRV 2838 GATATC GAT^ATC 3122 GATATC HaeII 1525 GGCGCT [GA]GCGC^[CT] 2749 AGCGCC HapII 1531 CCGG C^CGG 2126 CCGG HpaII 1531 CCGG C^CGG 2126 CCGG MspI 1531 CCGG C^CGG 2126 CCGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII