##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI ApaI AscI AseI
AsnI Asp718I AvaI AviII AvrII
BamHI BbrPI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 2656 CTTAAG C^TTAAG
AocI 1774 CCTCAGG CC^T[ACGT]AGG
Asp700I 3112 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1355 TGGCCA TGG^CCA
BanI 3288 GGCGCC G^G[CT][GA]CC
BbeI 3288 GGCGCC GGCGC^C
BbvCI 2154 GCTGAGG CCTCAGC(-5/-2)
BcgI 2616 CGACAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2656 CTTAAG C^TTAAG
BglI 2629 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 464 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 464 GCTCAGC GC^T[ACGT]AGC
BseRI 397 CTCCTC GAGGAG(10/8)
BspHI 2995 TCATGA T^CATGA
Bsu36I 1774 CCTCAGG CC^T[ACGT]AGG
CelII 464 GCTCAGC GC^T[ACGT]AGC
DraIII 3081 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1774 CCTCAGG CC^T[ACGT]AGG
EcoNI 3301 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 350 GATATC GAT^ATC
EcoT22I 1476 ATGCAT ATGCA^T
FauI 2304 CCCGC CCCGC(4/6)
HaeII 3288 GGCGCC [GA]GCGC^[CT]
KasI 3288 GGCGCC G^GCGCC
MluNI 1355 TGGCCA TGG^CCA
MscI 1355 TGGCCA TGG^CCA
NarI 3288 GGCGCC GG^CGCC
NheI 1029 GCTAGC G^CTAGC
NsiI 1476 ATGCAT ATGCA^T
PflFI 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3403 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2995 TCATGA T^CATGA
ScaI 2931 AGTACT AGT^ACT
SexAI 3306 ACCAGGT A^CC[AT]GGT
SfoI 3288 GGCGCC GGC^GCC
SphI 1001 GCATGC GCATG^C
SspI 1497 AATATT AAT^ATT
StuI 2601 AGGCCT AGG^CCT
Tth111I 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3112 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACTCGAAGTC
Alw44I 2276 GTGCAC G^TGCAC
3385 GTGCAC
ApaLI 2276 GTGCAC G^TGCAC
3385 GTGCAC
AspEI 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACTCGAAGTC
BciVI 2782 GGATAC GTATCC(6/5)
707 GTATCC
BclI 1157 TGATCA T^GATCA
1445 TGATCA
Bsp1407I 3546 TGTACA T^GTACA
3572 TGTACA
BspMI 582 GCAGGT ACCTGC(4/8)
2821 GCAGGT
BsrGI 3546 TGTACA T^GTACA
3572 TGTACA
DraI 2017 TTTAAA TTT^AAA
3584 TTTAAA
DraII 674 AGGACCT [GA]G^G[ACGT]CC[CT]
2793 AGGACCT
EaeI 719 TGGCCG [CT]^GGCC[GA]
1355 TGGCCA
Eam1105I 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2667 GACTCGAAGTC
EcoO109I 674 AGGACCT [GA]G^G[ACGT]CC[CT]
2793 AGGACCT
FbaI 1157 TGATCA T^GATCA
1445 TGATCA
HincII 237 GTCAAC GT[CT]^[GA]AC
457 GTCAAC
HindII 237 GTCAAC GT[CT]^[GA]AC
457 GTCAAC
HindIII 134 AAGCTT A^AGCTT
3529 AAGCTT
NdeI 215 CATATG CA^TATG
3566 CATATG
PpuMI 674 AGGACCT [GA]G^G[AT]CC[CT]
2793 AGGACCT
PvuII 1540 CAGCTG CAG^CTG
1793 CAGCTG
SapI 481 GAAGAGC GCTCTTC(1/4)
1630 GCTCTTC
SspBI 3546 TGTACA T^GTACA
3572 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII