################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbrPI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflII 2656 CTTAAG C^TTAAG AocI 1774 CCTCAGG CC^T[ACGT]AGG Asp700I 3112 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1355 TGGCCA TGG^CCA BanI 3288 GGCGCC G^G[CT][GA]CC BbeI 3288 GGCGCC GGCGC^C BbvCI 2154 GCTGAGG CCTCAGC(-5/-2) BcgI 2616 CGACAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 2656 CTTAAG C^TTAAG BglI 2629 GCCACCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 464 GCTCAGC GC^T[ACGT]AGC Bpu1102I 464 GCTCAGC GC^T[ACGT]AGC BseRI 397 CTCCTC GAGGAG(10/8) BspHI 2995 TCATGA T^CATGA Bsu36I 1774 CCTCAGG CC^T[ACGT]AGG CelII 464 GCTCAGC GC^T[ACGT]AGC DraIII 3081 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1774 CCTCAGG CC^T[ACGT]AGG EcoNI 3301 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 350 GATATC GAT^ATC EcoT22I 1476 ATGCAT ATGCA^T FauI 2304 CCCGC CCCGC(4/6) HaeII 3288 GGCGCC [GA]GCGC^[CT] KasI 3288 GGCGCC G^GCGCC MluNI 1355 TGGCCA TGG^CCA MscI 1355 TGGCCA TGG^CCA NarI 3288 GGCGCC GG^CGCC NheI 1029 GCTAGC G^CTAGC NsiI 1476 ATGCAT ATGCA^T PflFI 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3403 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2995 TCATGA T^CATGA ScaI 2931 AGTACT AGT^ACT SexAI 3306 ACCAGGT A^CC[AT]GGT SfoI 3288 GGCGCC GGC^GCC SphI 1001 GCATGC GCATG^C SspI 1497 AATATT AAT^ATT StuI 2601 AGGCCT AGG^CCT Tth111I 1582 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3112 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AhdI 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2667 GACTCGAAGTC Alw44I 2276 GTGCAC G^TGCAC 3385 GTGCAC ApaLI 2276 GTGCAC G^TGCAC 3385 GTGCAC AspEI 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2667 GACTCGAAGTC BciVI 2782 GGATAC GTATCC(6/5) 707 GTATCC BclI 1157 TGATCA T^GATCA 1445 TGATCA Bsp1407I 3546 TGTACA T^GTACA 3572 TGTACA BspMI 582 GCAGGT ACCTGC(4/8) 2821 GCAGGT BsrGI 3546 TGTACA T^GTACA 3572 TGTACA DraI 2017 TTTAAA TTT^AAA 3584 TTTAAA DraII 674 AGGACCT [GA]G^G[ACGT]CC[CT] 2793 AGGACCT EaeI 719 TGGCCG [CT]^GGCC[GA] 1355 TGGCCA Eam1105I 2259 GACGGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2667 GACTCGAAGTC EcoO109I 674 AGGACCT [GA]G^G[ACGT]CC[CT] 2793 AGGACCT FbaI 1157 TGATCA T^GATCA 1445 TGATCA HincII 237 GTCAAC GT[CT]^[GA]AC 457 GTCAAC HindII 237 GTCAAC GT[CT]^[GA]AC 457 GTCAAC HindIII 134 AAGCTT A^AGCTT 3529 AAGCTT NdeI 215 CATATG CA^TATG 3566 CATATG PpuMI 674 AGGACCT [GA]G^G[AT]CC[CT] 2793 AGGACCT PvuII 1540 CAGCTG CAG^CTG 1793 CAGCTG SapI 481 GAAGAGC GCTCTTC(1/4) 1630 GCTCTTC SspBI 3546 TGTACA T^GTACA 3572 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII