##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AscI AseI AsnI Asp718I BbeI
BbrPI BcgI BciVI BclI BlpI
Bpu1102I BsaBI BsaI BseAI BsiWI
BspEI BssHII Bst1107I BstBI BstUI
BstZ17I CelII CpoI DraIII EcoRI
FbaI FseI KasI KpnI KspI
MamI MluI MroI MvnI NaeI
NarI NgoMIV NotI NruI NspV
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SpeI Sse8387I SspI StuI
SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 461 GACGTC GACGT^C
AflII 2104 CTTAAG C^TTAAG
AflIII 1320 ACATGT A^C[GA][CT]GT
AgeI 1961 ACCGGT A^CCGGT
AhdI 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 184 GTGCAC G^TGCAC
Aor51HI 416 AGCGCT AGC^GCT
ApaLI 184 GTGCAC G^TGCAC
Asp700I 3040 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 248 CCTAGG C^CTAGG
BfrI 2104 CTTAAG C^TTAAG
BglI 3605 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 996 AGATCT A^GATCT
BlnI 248 CCTAGG C^CTAGG
BsaAI 3050 TACGTA [CT]AC^GT[GA]
BsaWI 1961 ACCGGT [AT]^CCGG[AT]
BsiEI 1358 CGGCCG CG[GA][CT]^CG
BsmBI 4020 CGTCTC CGTCTC(1/5)
BspDI 1607 ATCGAT AT^CGAT
BspHI 1315 TCATGA T^CATGA
BspLU11I 1320 ACATGT A^CATGT
BstXI 1297 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1607 ATCGAT AT^CGAT
DraI 2393 TTTAAA TTT^AAA
DrdI 392 GACCTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1358 CGGCCG C^GGCCG
Eam1105I 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1358 CGGCCG C^GGCCG
Eco47III 416 AGCGCT AGC^GCT
Eco52I 1358 CGGCCG C^GGCCG
HindIII 1727 AAGCTT A^AGCTT
HpaI 4284 GTTAAC GTT^AAC
MfeI 4096 CAATTG C^AATTG
MunI 4096 CAATTG C^AATTG
NheI 2145 GCTAGC G^CTAGC
PacI 2218 TTAATTAA TTAAT^TAA
PaeR7I 803 CTCGAG C^TCGAG
PflFI 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1961 ACCGGT A^CCGGT
PpuMI 1797 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1136 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1315 TCATGA T^CATGA
SapI 3394 GCTCTTC GCTCTTC(1/4)
SfiI 3604 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SnaBI 3050 TACGTA TAC^GTA
Tth111I 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1268 TCTAGA T^CTAGA
XcmI 3102 CCATTGGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 803 CTCGAG C^TCGAG
XmnI 3040 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 461 GACGTC G[GA]^CG[CT]C
4018 GGCGTC
AocI 1793 CCTGAGG CC^T[ACGT]AGG
3119 CCTGAGG
ApaI 2119 GGGCCC GGGCC^C
2429 GGGCCC
AviII 1564 TGCGCA TGC^GCA
3623 TGCGCA
BalI 1095 TGGCCA TGG^CCA
3612 TGGCCA
BamHI 2553 GGATCC G^GATCC
2809 GGATCC
BanI 1987 GGCACC G^G[CT][GA]CC
3782 GGCACC
BbvCI 192 CCTCAGC CCTCAGC(-5/-2)
1174 CCTCAGC
BcnI 145 CCCGG CC^[GC]GG
1900 CCGGG
BsaHI 461 GACGTC G[GA]^CG[CT]C
4018 GGCGTC
BsgI 1881 CTGCAC GTGCAG(16/14)
3126 GTGCAG
BsrFI 1356 ACCGGC [GA]^CCGG[CT]
1961 ACCGGT
BssSI 403 CACGAG CACGAG(-5/-1)
1124 CACGAG
BstEII 3086 GGTTACC G^GT[ACGT]ACC
3356 GGTGACC
BstPI 3086 GGTTACC G^GT[ACGT]ACC
3356 GGTGACC
Bsu36I 1793 CCTGAGG CC^T[ACGT]AGG
3119 CCTGAGG
Cfr10I 1356 ACCGGC [GA]^CCGG[CT]
1961 ACCGGT
DraII 1797 AGGACCT [GA]G^G[ACGT]CC[CT]
2429 GGGCCCT
Eco81I 1793 CCTGAGG CC^T[ACGT]AGG
3119 CCTGAGG
EcoNI 248 CCTAGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3522 CCTCTTGGAGG
EcoO109I 1797 AGGACCT [GA]G^G[ACGT]CC[CT]
2429 GGGCCCT
EcoO65I 3086 GGTTACC G^GT[ACGT]ACC
3356 GGTGACC
EcoT22I 2283 ATGCAT ATGCA^T
3868 ATGCAT
FspI 1564 TGCGCA TGC^GCA
3623 TGCGCA
HgaI 919 GCGTC GACGC(5/10)
4019 GCGTC
Hin1I 461 GACGTC G[GA]^CG[CT]C
4018 GGCGTC
MluNI 1095 TGGCCA TGG^CCA
3612 TGGCCA
MscI 1095 TGGCCA TGG^CCA
3612 TGGCCA
NciI 145 CCCGG CC^[GC]GG
1900 CCGGG
NdeI 2328 CATATG CA^TATG
2618 CATATG
NsiI 2283 ATGCAT ATGCA^T
3868 ATGCAT
SphI 167 GCATGC GCATG^C
3866 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII