################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AscI AseI AsnI Asp718I BbeI BbrPI BcgI BciVI BclI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BspEI BssHII Bst1107I BstBI BstUI BstZ17I CelII CpoI DraIII EcoRI FbaI FseI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NgoMIV NotI NruI NspV PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspI StuI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 461 GACGTC GACGT^C AflII 2104 CTTAAG C^TTAAG AflIII 1320 ACATGT A^C[GA][CT]GT AgeI 1961 ACCGGT A^CCGGT AhdI 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 184 GTGCAC G^TGCAC Aor51HI 416 AGCGCT AGC^GCT ApaLI 184 GTGCAC G^TGCAC Asp700I 3040 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 248 CCTAGG C^CTAGG BfrI 2104 CTTAAG C^TTAAG BglI 3605 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 996 AGATCT A^GATCT BlnI 248 CCTAGG C^CTAGG BsaAI 3050 TACGTA [CT]AC^GT[GA] BsaWI 1961 ACCGGT [AT]^CCGG[AT] BsiEI 1358 CGGCCG CG[GA][CT]^CG BsmBI 4020 CGTCTC CGTCTC(1/5) BspDI 1607 ATCGAT AT^CGAT BspHI 1315 TCATGA T^CATGA BspLU11I 1320 ACATGT A^CATGT BstXI 1297 CCAGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1607 ATCGAT AT^CGAT DraI 2393 TTTAAA TTT^AAA DrdI 392 GACCTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1358 CGGCCG C^GGCCG Eam1105I 4107 GACTTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1358 CGGCCG C^GGCCG Eco47III 416 AGCGCT AGC^GCT Eco52I 1358 CGGCCG C^GGCCG HindIII 1727 AAGCTT A^AGCTT HpaI 4284 GTTAAC GTT^AAC MfeI 4096 CAATTG C^AATTG MunI 4096 CAATTG C^AATTG NheI 2145 GCTAGC G^CTAGC PacI 2218 TTAATTAA TTAAT^TAA PaeR7I 803 CTCGAG C^TCGAG PflFI 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1961 ACCGGT A^CCGGT PpuMI 1797 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1136 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1315 TCATGA T^CATGA SapI 3394 GCTCTTC GCTCTTC(1/4) SfiI 3604 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SnaBI 3050 TACGTA TAC^GTA Tth111I 311 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1268 TCTAGA T^CTAGA XcmI 3102 CCATTGGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 803 CTCGAG C^TCGAG XmnI 3040 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 461 GACGTC G[GA]^CG[CT]C 4018 GGCGTC AocI 1793 CCTGAGG CC^T[ACGT]AGG 3119 CCTGAGG ApaI 2119 GGGCCC GGGCC^C 2429 GGGCCC AviII 1564 TGCGCA TGC^GCA 3623 TGCGCA BalI 1095 TGGCCA TGG^CCA 3612 TGGCCA BamHI 2553 GGATCC G^GATCC 2809 GGATCC BanI 1987 GGCACC G^G[CT][GA]CC 3782 GGCACC BbvCI 192 CCTCAGC CCTCAGC(-5/-2) 1174 CCTCAGC BcnI 145 CCCGG CC^[GC]GG 1900 CCGGG BsaHI 461 GACGTC G[GA]^CG[CT]C 4018 GGCGTC BsgI 1881 CTGCAC GTGCAG(16/14) 3126 GTGCAG BsrFI 1356 ACCGGC [GA]^CCGG[CT] 1961 ACCGGT BssSI 403 CACGAG CACGAG(-5/-1) 1124 CACGAG BstEII 3086 GGTTACC G^GT[ACGT]ACC 3356 GGTGACC BstPI 3086 GGTTACC G^GT[ACGT]ACC 3356 GGTGACC Bsu36I 1793 CCTGAGG CC^T[ACGT]AGG 3119 CCTGAGG Cfr10I 1356 ACCGGC [GA]^CCGG[CT] 1961 ACCGGT DraII 1797 AGGACCT [GA]G^G[ACGT]CC[CT] 2429 GGGCCCT Eco81I 1793 CCTGAGG CC^T[ACGT]AGG 3119 CCTGAGG EcoNI 248 CCTAGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3522 CCTCTTGGAGG EcoO109I 1797 AGGACCT [GA]G^G[ACGT]CC[CT] 2429 GGGCCCT EcoO65I 3086 GGTTACC G^GT[ACGT]ACC 3356 GGTGACC EcoT22I 2283 ATGCAT ATGCA^T 3868 ATGCAT FspI 1564 TGCGCA TGC^GCA 3623 TGCGCA HgaI 919 GCGTC GACGC(5/10) 4019 GCGTC Hin1I 461 GACGTC G[GA]^CG[CT]C 4018 GGCGTC MluNI 1095 TGGCCA TGG^CCA 3612 TGGCCA MscI 1095 TGGCCA TGG^CCA 3612 TGGCCA NciI 145 CCCGG CC^[GC]GG 1900 CCGGG NdeI 2328 CATATG CA^TATG 2618 CATATG NsiI 2283 ATGCAT ATGCA^T 3868 ATGCAT SphI 167 GCATGC GCATG^C 3866 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII