##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BciVI BcnI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BsoBI
BspDI BspEI BsrFI BssHII BstBI
BstEII BstPI BstUI Bsu36I CelII
Cfr10I ClaI CpoI DraII DrdI
Eco47III Eco81I EcoO109I EcoO65I EcoRI
FseI FspI KasI KpnI KspI
MamI MluI MroI MspA1I MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SpeI Sse8387I SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AcyI 3475 GGCGTC G[GA]^CG[CT]C
AflIII 3164 ACATGT A^C[GA][CT]GT
Asp700I 401 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1887 GAGCTC G[AT]GC[AT]^C
BalI 3095 TGGCCA TGG^CCA
BanI 3078 GGTGCC G^G[CT][GA]CC
BbvCI 3511 CCTCAGC CCTCAGC(-5/-2)
BcgI 602 GCACATAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1255 TGATCA T^GATCA
BglI 896 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1488 TACGTA [CT]AC^GT[GA]
BsaHI 3475 GGCGTC G[GA]^CG[CT]C
BsaI 792 GGTCTC GGTCTC(1/5)
BsgI 600 CTGCAC GTGCAG(16/14)
BsiEI 1464 CGGCCG CG[GA][CT]^CG
BsiHKAI 1887 GAGCTC G[AT]GC[AT]^C
BsmBI 17 CGTCTC CGTCTC(1/5)
BsmI 4173 GAATGC GAATGC(1/-1)
BspHI 2843 TCATGA T^CATGA
BspLU11I 3164 ACATGT A^CATGT
BsrBI 61 CCGCTC CCGCTC(-3/-3)
Bst1107I 1543 GTATAC GTA^TAC
BstAPI 3767 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1904 CCAAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1543 GTATAC GTA^TAC
DraIII 3480 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1464 CGGCCG C^GGCCG
EclXI 1464 CGGCCG C^GGCCG
Eco52I 1464 CGGCCG C^GGCCG
EcoRV 295 GATATC GAT^ATC
EcoT22I 4175 ATGCAT ATGCA^T
FbaI 1255 TGATCA T^GATCA
HapII 77 CCGG C^CGG
Hin1I 3475 GGCGTC G[GA]^CG[CT]C
HincII 2497 GTTAAC GT[CT]^[GA]AC
HindII 2497 GTTAAC GT[CT]^[GA]AC
HindIII 2263 AAGCTT A^AGCTT
HpaI 2497 GTTAAC GTT^AAC
HpaII 77 CCGG C^CGG
MfeI 210 CAATTG C^AATTG
MluNI 3095 TGGCCA TGG^CCA
MscI 3095 TGGCCA TGG^CCA
MspI 77 CCGG C^CGG
MunI 210 CAATTG C^AATTG
NsiI 4175 ATGCAT ATGCA^T
PflMI 307 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2843 TCATGA T^CATGA
SacI 1887 GAGCTC GAGCT^C
SapI 63 GCTCTTC GCTCTTC(1/4)
ScaI 3435 AGTACT AGT^ACT
SnaBI 1488 TACGTA TAC^GTA
SphI 333 GCATGC GCATG^C
StuI 824 AGGCCT AGG^CCT
Van91I 307 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1102 CCATTACCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 401 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflII 2654 CTTAAG C^TTAAG
3538 CTTAAG
AhdI 731 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2052 GACATCATGTC
AlwNI 287 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
1505 CAGTTTCTG
AspEI 731 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2052 GACATCATGTC
AvaII 738 GGTCC G^G[AT]CC
1822 GGTCC
BfrI 2654 CTTAAG C^TTAAG
3538 CTTAAG
BglII 2865 AGATCT A^GATCT
4009 AGATCT
BseRI 22 CTCCTC GAGGAG(10/8)
1217 GAGGAG
EaeI 1464 CGGCCG [CT]^GGCC[GA]
3095 TGGCCA
Eam1105I 731 GACAGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2052 GACATCATGTC
EcoNI 492 CCTGCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
554 CCTTCGCAAGG
FauI 60 CCCGC CCCGC(4/6)
91 CCCGC
HaeII 2 GGCGCT [GA]GCGC^[CT]
116 GGCGCT
NcoI 751 CCATGG C^CATGG
3010 CCATGG
SspI 2441 AATATT AAT^ATT
3717 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI BanII
BbsI BbvI BfaI BmyI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII